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Rabbit Recombinant Monoclonal FLIP antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.

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Images

Western blot - Anti-FLIPs (short isoform) antibody [EPR29011-104] (AB323748), expandable thumbnail
  • Western blot - Anti-FLIPs (short isoform) antibody [EPR29011-104] (AB323748), expandable thumbnail
  • Western blot - Anti-FLIPs (short isoform) antibody [EPR29011-104] (AB323748), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Target data

Function

Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.

Alternative names

CASH, CASP8AP1, CLARP, MRIT, CASP8 and FADD-like apoptosis regulator, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, Inhibitor of FLICE, MACH-related inducer of toxicity, Usurpin, Casper, c-FLIP, FLAME-1, I-FLICE

Rabbit Recombinant Monoclonal FLIP antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29011-104
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-FLIPs (short isoform) antibody [EPR29011-104] (ab323748), expandable thumbnail

    Western blot - Anti-FLIPs (short isoform) antibody [EPR29011-104] (ab323748)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-FLIPs (short isoform) antibody [EPR29011-104] (ab323748) at 1/1000 dilution

    All lanes: Human pancreas cancer tissue lysate at 80 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 25 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-FLIPs (short isoform) antibody [EPR29011-104] (ab323748), expandable thumbnail

    Western blot - Anti-FLIPs (short isoform) antibody [EPR29011-104] (ab323748)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human FLIP-L.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution. Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution

    All lanes: Western blot - Anti-FLIPs (short isoform) antibody [EPR29011-104] (ab323748) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human FLIP-S expression vector containing a His-tag, whole cell lysate at 5 µg

    Lane 3: 293T cells transfected with a human FLIP-L expression vector containing a His-tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

    Observed band size: 25 kDa, 36 kDa, 25-50 kDa

    Exposure time: 3s

  • Western blot - Anti-FLIPs (short isoform) antibody [EPR29011-104] (ab323748), expandable thumbnail

    Western blot - Anti-FLIPs (short isoform) antibody [EPR29011-104] (ab323748)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: Daudi, NAMALWA (PMID: 15078899).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-FLIPs (short isoform) antibody [EPR29011-104] (ab323748) at 1/1000 dilution

    Lane 1: HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 80 µg

    Lane 2: Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 80 µg

    Lane 3: KARPAS-299 (human T cell lymphoma cell) whole cell lysate at 80 µg

    Lane 4: NAMALWA (human Burkitts lymphoma B lymphocyte) whole cell lysate at 80 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 25 kDa, 36 kDa

    Exposure time: 37s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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