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AB69400

Anti-FLJ35220 antibody

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(3 Publications)

Mouse Polyclonal Endonuclease V antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Endonuclease V, hEndoV, Inosine-specific endoribonuclease, ENDOV

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FLJ35220 antibody (AB69400)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FLJ35220 antibody (AB69400)

ab69400 at 10μg/ml staining FLJ35220 in Hela cells by ICC/IF.

Western blot - Anti-FLJ35220 antibody (AB69400)
  • WB

Unknown

Western blot - Anti-FLJ35220 antibody (AB69400)

All lanes:

Western blot - Anti-FLJ35220 antibody (ab69400) at 1/500 dilution

Lane 1:

FLJ35220 transfected 293T cell lysate at 25 µg

Lane 2:

Non transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Endoribonuclease that specifically cleaves inosine-containing RNAs : cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed : 23912683, PubMed : 23912718, PubMed : 25195743, PubMed : 27573237, PubMed : 31703097). Active against both single-stranded and double-stranded RNAs (PubMed : 25195743, PubMed : 31703097). Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs) (PubMed : 23912718). Cleaves mRNAs and tRNAs containing inosine (PubMed : 23912683, PubMed : 31703097). Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3' (PubMed : 23912718, PubMed : 27573237). Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear : it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing (Probable). Binds branched DNA structures (PubMed : 23139746).. Isoform 6. Endoribonuclease that specifically cleaves inosine-containing RNAs : cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed : 31703097). Active against both single-stranded and double-stranded RNAs (PubMed : 31703097). Cleaves tRNAs containing inosine (PubMed : 31703097).. Isoform 7. Endoribonuclease that specifically cleaves inosine-containing RNAs : cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed : 31703097). Active against both single-stranded and double-stranded RNAs (PubMed : 31703097). Cleaves tRNAs containing inosine (PubMed : 31703097).
See full target information ENDOV
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Heart Association 10:e020656 PubMed34259011

2021

Regulates Atherosclerosis Through C-C Motif Chemokine Ligand 2-Mediated Monocyte Infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Yi Kong,Camilla Huse,Kuan Yang,Jonas Øgaard,Natalia Berges,Erik Sebastian Vik,Meh Sameen Nawaz,Ana Quiles-Jiménez,Azhar Abbas,Ida Gregersen,Sverre Holm,Vigdis Bjerkli,Azita Rashidi,Cathrine Fladeby,Rajikala Suganthan,Ellen Lund Sagen,Mona Skjelland,Anna Lång,Stig Ove Bøe,Magnar Bjørås,Pål Aukrust,Ingrun Alseth,Bente Halvorsen,Tuva Børresdatter Dahl

PloS one 14:e0225081 PubMed31703097

2019

Complex alternative splicing of human Endonuclease V mRNA, but evidence for only a single protein isoform.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Berges,Meh Sameen Nawaz,Tuva Børresdatter Dahl,Lars Hagen,Magnar Bjørås,Jon K Laerdahl,Ingrun Alseth

The Journal of biological chemistry 291:21786-21801 PubMed27573237

2016

Regulation of Human Endonuclease V Activity and Relocalization to Cytoplasmic Stress Granules.

Applications

Unspecified application

Species

Unspecified reactive species

Meh Sameen Nawaz,Erik Sebastian Vik,Natalia Berges,Cathrine Fladeby,Magnar Bjørås,Bjørn Dalhus,Ingrun Alseth
View all publications
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Product promise

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