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Mouse Monoclonal TUP1 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 1 publication.


Images

Western blot - Anti-FLK1 antibody [Clone 10] (AB24313), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Mouse
Storage buffer

Preservative: 0.08% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Saccharomyces cerevisiae
Tested

Tested
Tested

Species
Saccharomyces cerevisiae
Dilution info
5.00000-10.00000 µg/mL
Notes

-

Target data

Function

Acts as a component of the CYC8-TUP1 corepressor complex which is involved in the repression of many genes in a wide variety of physiological processes including heme-regulated and catabolite repressed genes. May also be involved in the derepression of at least some target genes. The complex is recruited to target genes by interaction with DNA-bound transcriptional repressors, like MATALPHA2, MIG1, RFX1 and SKO1. The complex recruits histone deacetylases to produce a repressive chromatin structure, interacts with hypoacetylated N-terminal tails of histones H3 and H4 that have been programmed for repression by the action of histone deacetylases and interferes directly with the transcriptional machinery by associating with the RNA polymerase II mediator complex.

Alternative names

Recommended products

Mouse Monoclonal TUP1 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Clone number
Clone 10
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-FLK1 antibody [Clone 10] (ab24313), expandable thumbnail
    Image is courtesy of an anonymous customer review.

    Western blot - Anti-FLK1 antibody [Clone 10] (ab24313)

    All lanes: Western blot - Anti-FLK1 antibody [Clone 10] (ab24313)

    All lanes: Saccharomyces cerevisiae whole cell lysates at 15 µg

    Secondary

    All lanes: Sheep anti-Mouse polyclonal HRP conjugate at 100 %

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 78 kDa

    Observed band size: 90 kDa

    Exposure time: 1min

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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