Goat Polyclonal Flotillin 1 antibody. Suitable for WB and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human FLOT1 aa 400 to C-terminus.
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- Cell & bioscience 12:1542022QKI degradation in macrophage by RNF6 protects mice from MRSA infection via enhancing PI3K p110β dependent autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongsheng Zhai,Wenwen Wang,Zichen Ye,Ke Xue,Guo Chen,Sijun Hu,Zhao Yan,Yanhai Guo,Fang Wang,Xubo Li,An Xiang,Xia Li,Zifan Lu,Li WangPubMed 36088389
- Scientific reports 12:4962022A new transgene mouse model using an extravesicular EGFP tag enables affinity isolation of cell-specific extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMikkel Ø Nørgård,Lasse B Steffensen,Didde R Hansen,Ernst-Martin Füchtbauer,Morten B Engelund,Henrik Dimke,Ditte C Andersen,Per SvenningsenPubMed 35017633
- Redox biology 48:1021932021TRAIL sensitivity of nasopharyngeal cancer cells involves redox dependent upregulation of TMTC2 and its interaction with membrane caspase-3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepika Raman,Patricia Tay,Jayshree L Hirpara,Dan Liu,Shazib PervaizPubMed 34839142
- Experimental and therapeutic medicine 22:14372021Diagnostic value of using exosome-derived cysteine-rich protein 61 as biomarkers for acute coronary syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Li,Yi Li,Wei Zhi,Chen Liu,Weize Fan,Qing Miao,Xinshun GuPubMed 34721679
- Biomarker insights 16:117727192110182042021Extracellular Vesicle Proteome of Breast Cancer Patients with and Without Cognitive Impairment Following Anthracycline-based Chemotherapy: An Exploratory Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Qin Koh,Ding Quan Ng,Chiu Chin Ng,Adrian Boey,Meng Wei,Siu Kwan Sze,Han Kiat Ho,Munjal Acharya,Charles L Limoli,Alexandre ChanPubMed 34103887
- Science translational medicine 12:2020Cardiosphere-derived exosomal microRNAs for myocardial repair in pediatric dilated cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKenta Hirai,Daiki Ousaka,Yosuke Fukushima,Maiko Kondo,Takahiro Eitoku,Yusuke Shigemitsu,Mayuko Hara,Kenji Baba,Tatsuo Iwasaki,Shingo Kasahara,Shinichi Ohtsuki,Hidemasa OhPubMed 33298561
- Cell reports 32:1080172020Flotillin-Dependent Membrane Microdomains Are Required for Functional Phagolysosomes against Fungal Infections.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFranziska Schmidt,Andreas Thywißen,Marie Goldmann,Cristina Cunha,Zoltán Cseresnyés,Hella Schmidt,Muhammad Rafiq,Silvia Galiani,Markus H Gräler,Georgios Chamilos,João F Lacerda,António Campos,Christian Eggeling,Marc Thilo Figge,Thorsten Heinekamp,Scott G Filler,Agostinho Carvalho,Axel A BrakhagePubMed 32814035
- Journal of nutrition and metabolism 2019:57647402019A Method for the Isolation of Exosomes from Human and Bovine Milk.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKanchan Vaswani,Murray D Mitchell,Olivia J Holland,Yong Qin Koh,Rebecca J Hill,Tracy Harb,Peter S W Davies,Hassendrini PeirisPubMed 31885909
- Frontiers in immunology 10:19872019Airway Epithelial Cells Generate Pro-inflammatory Tenascin-C and Small Extracellular Vesicles in Response to TLR3 Stimuli and Rhinovirus Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJake T Mills et. al.PubMed 31497021
- PLoS pathogens 10:e10039412014CIB1 synergizes with EphrinA2 to regulate Kaposi's sarcoma-associated herpesvirus macropinocytic entry in human microvascular dermal endothelial cells.
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human FLOT1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O75955
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Chimpanzee | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Pig | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.10000-0.30000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig, Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Alternative names
Flotillin-1, FLOT1
Recommended products
Goat Polyclonal Flotillin 1 antibody. Suitable for WB and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human FLOT1 aa 400 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human FLOT1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O75955
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Flotillin 1 also known as flotillin-1 or flot-1 functions as a major scaffolding protein in lipid rafts which are specialized membrane microdomains. It has a molecular weight of approximately 47 kDa facilitating protein interactions within the cell membrane. Flotillin 1 localizes primarily to non-caveolar lipid rafts and is expressed widely across various tissue types. High expression levels occur in brain tissue contributing to its recognition as an important element within neuronal membranes.
- Biological function summary
Flotillin-1 participates in vesicle trafficking and endocytosis by forming a complex with its counterpart flotillin-2. This complex stabilizes membrane microdomains and assists in signaling pathways. Flotillin-1 plays a significant role in cell adhesion migration and cytoskeleton organization by interacting with actin and other cytoskeletal proteins. By doing so it influences cellular processes related to membrane shaping and intracellular transport.
- Pathways
Flotillin-1 integrates into the MAPK signaling pathway and the Wnt signaling pathway which are critical for cell differentiation and proliferation. It interacts with several proteins including caveolin-1 that have known roles in these pathways. These interactions help modulate signaling cascades providing necessary cellular responses to various extracellular stimuli.
- Associated diseases and disorders
Flotillin-1 has associations with Alzheimer's disease and various cancers. In Alzheimer's disease it interacts with amyloid precursor protein influencing amyloid-beta production and deposition. In cancer altered expression levels of flotillin-1 affect tumor cell adhesion and migration with its interactions impacting the expression of proteins like E-cadherin which is associated with cancer metastasis and progression. Understanding these interactions offers potential insights for developing therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Flotillin 1 antibody (ab13493)
Detected by chemiluminescence.
All lanes: Western blot - Anti-Flotillin 1 antibody (ab13493) at 0.1 µg/mL
All lanes: K562 cell lysate (RIPA buffer) at 35 µg
Predicted band size: 47 kDa
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