AB13493

Anti-Flotillin 1 antibody

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(18 Publications)

Goat Polyclonal Flotillin 1 antibody. Suitable for WB and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human FLOT1 aa 400 to C-terminus.

View Alternative Names

Flotillin-1, FLOT1

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

Immunogen

Synthetic Peptide within Human FLOT1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Flotillin 1 also known as flotillin-1 or flot-1 functions as a major scaffolding protein in lipid rafts which are specialized membrane microdomains. It has a molecular weight of approximately 47 kDa facilitating protein interactions within the cell membrane. Flotillin 1 localizes primarily to non-caveolar lipid rafts and is expressed widely across various tissue types. High expression levels occur in brain tissue contributing to its recognition as an important element within neuronal membranes.

Biological function summary

Flotillin-1 participates in vesicle trafficking and endocytosis by forming a complex with its counterpart flotillin-2. This complex stabilizes membrane microdomains and assists in signaling pathways. Flotillin-1 plays a significant role in cell adhesion migration and cytoskeleton organization by interacting with actin and other cytoskeletal proteins. By doing so it influences cellular processes related to membrane shaping and intracellular transport.

Pathways

Flotillin-1 integrates into the MAPK signaling pathway and the Wnt signaling pathway which are critical for cell differentiation and proliferation. It interacts with several proteins including caveolin-1 that have known roles in these pathways. These interactions help modulate signaling cascades providing necessary cellular responses to various extracellular stimuli.

Flotillin-1 has associations with Alzheimer's disease and various cancers. In Alzheimer's disease it interacts with amyloid precursor protein influencing amyloid-beta production and deposition. In cancer altered expression levels of flotillin-1 affect tumor cell adhesion and migration with its interactions impacting the expression of proteins like E-cadherin which is associated with cancer metastasis and progression. Understanding these interactions offers potential insights for developing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.

See full target information FLOT1

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed40002894

2025

SNX19 Interacts with Caveolin-1 and Flotillin-1 to Regulate DR Endocytosis and Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bibhas Amatya,Jacob Q M Polzin,Van A M Villar,Jiang Yang,Prasad Konkalmatt,Xiaoyan Wang,Raisha C Cadme,Peng Xu,John J Gildea,Santiago Cuevas,Ines Armando,Robin A Felder,Pedro A Jose,Hewang Lee

FASEB bioAdvances 5:355-366 PubMed37674540

2023

Mitochondrial reactive oxygen species modify extracellular vesicles secretion rate.

Applications

Unspecified application

Species

Unspecified reactive species

Mikkel Ø Nørgård,Philip M Lund,Nazmie Kalisi,Thomas L Andresen,Jannik B Larsen,Stefan Vogel,Per Svenningsen

Cell & bioscience 12:154 PubMed36088389

2022

QKI degradation in macrophage by RNF6 protects mice from MRSA infection via enhancing PI3K p110β dependent autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Zhai,Wenwen Wang,Zichen Ye,Ke Xue,Guo Chen,Sijun Hu,Zhao Yan,Yanhai Guo,Fang Wang,Xubo Li,An Xiang,Xia Li,Zifan Lu,Li Wang

Scientific reports 12:496 PubMed35017633

2022

A new transgene mouse model using an extravesicular EGFP tag enables affinity isolation of cell-specific extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Mikkel Ø Nørgård,Lasse B Steffensen,Didde R Hansen,Ernst-Martin Füchtbauer,Morten B Engelund,Henrik Dimke,Ditte C Andersen,Per Svenningsen

Redox biology 48:102193 PubMed34839142

2021

TRAIL sensitivity of nasopharyngeal cancer cells involves redox dependent upregulation of TMTC2 and its interaction with membrane caspase-3.

Applications

Unspecified application

Species

Unspecified reactive species

Deepika Raman,Patricia Tay,Jayshree L Hirpara,Dan Liu,Shazib Pervaiz

Experimental and therapeutic medicine 22:1437 PubMed34721679

2021

Diagnostic value of using exosome-derived cysteine-rich protein 61 as biomarkers for acute coronary syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Yi Li,Wei Zhi,Chen Liu,Weize Fan,Qing Miao,Xinshun Gu

Biomarker insights 16:11772719211018204 PubMed34103887

2021

Extracellular Vesicle Proteome of Breast Cancer Patients with and Without Cognitive Impairment Following Anthracycline-based Chemotherapy: An Exploratory Study.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Qin Koh,Ding Quan Ng,Chiu Chin Ng,Adrian Boey,Meng Wei,Siu Kwan Sze,Han Kiat Ho,Munjal Acharya,Charles L Limoli,Alexandre Chan

Science translational medicine 12: PubMed33298561

2020

Cardiosphere-derived exosomal microRNAs for myocardial repair in pediatric dilated cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Kenta Hirai,Daiki Ousaka,Yosuke Fukushima,Maiko Kondo,Takahiro Eitoku,Yusuke Shigemitsu,Mayuko Hara,Kenji Baba,Tatsuo Iwasaki,Shingo Kasahara,Shinichi Ohtsuki,Hidemasa Oh

Cell reports 32:108017 PubMed32814035

2020

Flotillin-Dependent Membrane Microdomains Are Required for Functional Phagolysosomes against Fungal Infections.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska Schmidt,Andreas Thywißen,Marie Goldmann,Cristina Cunha,Zoltán Cseresnyés,Hella Schmidt,Muhammad Rafiq,Silvia Galiani,Markus H Gräler,Georgios Chamilos,João F Lacerda,António Campos,Christian Eggeling,Marc Thilo Figge,Thorsten Heinekamp,Scott G Filler,Agostinho Carvalho,Axel A Brakhage

Journal of nutrition and metabolism 2019:5764740 PubMed31885909

2019

A Method for the Isolation of Exosomes from Human and Bovine Milk.

Applications

Unspecified application

Species

Unspecified reactive species

Kanchan Vaswani,Murray D Mitchell,Olivia J Holland,Yong Qin Koh,Rebecca J Hill,Tracy Harb,Peter S W Davies,Hassendrini Peiris

View all publications

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