Rabbit Polyclonal Flotillin 1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 88 publications.
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- Atherosclerosis plus 53:16-252023Increased extracellular vesicles (EVs) related to T cell-mediated inflammation and vascular function in familial hypercholesterolemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMorten Hjuler Nielsen,Rikke Bæk,Malene Moller Jorgensen,Maiken Mellergaard,Aase HandbergPubMed 37637934
- Cells 12:2023Prognostic Role of Soluble and Extracellular Vesicle-Associated PD-L1, B7-H3 and B7-H4 in Non-Small Cell Lung Cancer Patients Treated with Immune Checkpoint Inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlo Genova,Roberta Tasso,Alessandra Rosa,Giovanni Rossi,Daniele Reverberi,Vincenzo Fontana,Silvia Marconi,Michela Croce,Maria Giovanna Dal Bello,Chiara Dellepiane,Marco Tagliamento,Maria Chiara Ciferri,Lodovica Zullo,Alessandro Fedeli,Angela Alama,Katia Cortese,Chiara Gentili,Eugenia Cella,Giorgia Anselmi,Marco Mora,Giulia Barletta,Erika Rijavec,Francesco Grossi,Paolo Pronzato,Simona CocoPubMed 36980174
- Journal of neuroinflammation 20:432023Amyloid-β accumulation in human astrocytes induces mitochondrial disruption and changed energy metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarlena Zyśk,Chiara Beretta,Luana Naia,Abdulkhalek Dakhel,Linnea Påvénius,Hjalmar Brismar,Maria Lindskog,Maria Ankarcrona,Anna ErlandssonPubMed 36803838
- International journal of molecular sciences 24:2023miRNA-124 Prevents Rat Diabetic Retinopathy by Inhibiting the Microglial Inflammatory Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Chen,Andrea Schlotterer,Luke Kurowski,Lin Li,Marcus Dannehl,Hans-Peter Hammes,Jihong LinPubMed 36768614
- Cells 11:2022Preconditioned Mesenchymal Stromal Cell-Derived Extracellular Vesicles (EVs) Counteract Inflammaging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCansu Gorgun,Chiara Africano,Maria Chiara Ciferri,Nadia Bertola,Daniele Reverberi,Rodolfo Quarto,Silvia Ravera,Roberta TassoPubMed 36429124
- Nature communications 13:50322022Pneumolysin boosts the neuroinflammatory response to Streptococcus pneumoniae through enhanced endocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSabrina Hupp,Christina Förtsch,Franziska Graber,Timothy J Mitchell,Asparouh I IlievPubMed 36028511
- PNAS nexus 1:pgac1732022Skeletal muscle releases extracellular vesicles with distinct protein and microRNA signatures that function in the muscle microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSho Watanabe,Yuri Sudo,Takumi Makino,Satoshi Kimura,Kenji Tomita,Makoto Noguchi,Hidetoshi Sakurai,Makoto Shimizu,Yu Takahashi,Ryuichiro Sato,Yoshio YamauchiPubMed 36714847
- International journal of molecular sciences 23:2022Effects of Dietary n-3 LCPUFA Supplementation on the Hippocampus of Aging Female Mice: Impact on Memory, Lipid Raft-Associated Glutamatergic Receptors and Neuroinflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucas Taoro-González,Daniel Pereda,Catalina Valdés-Baizabal,Miriam González-Gómez,José A Pérez,Fátima Mesa-Herrera,Ana Canerina-Amaro,Herminia Pérez-González,Covadonga Rodríguez,Mario Díaz,Raquel MarinPubMed 35806435
- Brain pathology (Zurich, Switzerland) 32:e130782022Accumulation of misfolded SOD1 outlines distinct patterns of motor neuron pathology and death during disease progression in a SOD1 mouse model of amyotrophic lateral sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Salvany,Anna Casanovas,Lídia Piedrafita,Sílvia Gras,Jordi Calderó,Josep E EsquerdaPubMed 35584812
- International journal of molecular sciences 23:2022Isolation of Platelet-Derived Exosomes from Human Platelet-Rich Plasma: Biochemical and Morphological Characterization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiquel Saumell-Esnaola,Diego Delgado,Gontzal García Del Caño,Maider Beitia,Joan Sallés,Imanol González-Burguera,Pello Sánchez,Maider López de Jesús,Sergio Barrondo,Mikel SánchezPubMed 35270001
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Dog | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes Fix cells with methanol |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Alternative names
Flotillin-1, FLOT1
Recommended products
Rabbit Polyclonal Flotillin 1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 88 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
This target has been reported to show both cytoplasmic and nuclear localisation (PMID: 15713644). From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab133497.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Flotillin 1 also known as flotillin-1 or flot-1 functions as a major scaffolding protein in lipid rafts which are specialized membrane microdomains. It has a molecular weight of approximately 47 kDa facilitating protein interactions within the cell membrane. Flotillin 1 localizes primarily to non-caveolar lipid rafts and is expressed widely across various tissue types. High expression levels occur in brain tissue contributing to its recognition as an important element within neuronal membranes.
- Biological function summary
Flotillin-1 participates in vesicle trafficking and endocytosis by forming a complex with its counterpart flotillin-2. This complex stabilizes membrane microdomains and assists in signaling pathways. Flotillin-1 plays a significant role in cell adhesion migration and cytoskeleton organization by interacting with actin and other cytoskeletal proteins. By doing so it influences cellular processes related to membrane shaping and intracellular transport.
- Pathways
Flotillin-1 integrates into the MAPK signaling pathway and the Wnt signaling pathway which are critical for cell differentiation and proliferation. It interacts with several proteins including caveolin-1 that have known roles in these pathways. These interactions help modulate signaling cascades providing necessary cellular responses to various extracellular stimuli.
- Associated diseases and disorders
Flotillin-1 has associations with Alzheimer's disease and various cancers. In Alzheimer's disease it interacts with amyloid precursor protein influencing amyloid-beta production and deposition. In cancer altered expression levels of flotillin-1 affect tumor cell adhesion and migration with its interactions impacting the expression of proteins like E-cadherin which is associated with cancer metastasis and progression. Understanding these interactions offers potential insights for developing therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
Western blot - Anti-Flotillin 1 antibody (ab41927)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: Flotillin 1 knockout HAP1 whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - ab41927 observed at 47 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
ab41927 was shown to specifically react with Flotillin 1 in wild-type HAP1 cells as signal was lost in Flotillin 1 knockout cells. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. ab41927 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Flotillin 1 antibody (ab41927)
Predicted band size: 47 kDa
This image is part of a customer review submitted by Dr. Kirk McManusImmunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody (ab41927)
ab41927 (1/200) staining Flotillin 1 in HeLa cells (green). Cells were fixed with Methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
Western blot - Anti-Flotillin 1 antibody (ab41927)
All lanes: Western blot - Anti-Flotillin 1 antibody (ab41927) at 1 µg/mL
Lane 1: Lung (Human) Tissue Lysate at 10 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3: Human brain tissue lysate - total protein (ab29466) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody (ab41927)
ICC/IF image of ab41927 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab41927 at 1μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Western blot - Anti-Flotillin 1 antibody (ab41927)
All lanes: Western blot - Anti-Flotillin 1 antibody (ab41927) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Human brain tissue lysate - total protein (ab29466) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 35 kDa, 50 kDa
Exposure time: 4min
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