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Rabbit Recombinant Monoclonal Flotillin 1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Flotillin 1 antibody [EP446Y] (AB78178), expandable thumbnail
  • Western blot - Anti-Flotillin 1 antibody [EP446Y] (AB78178), expandable thumbnail
  • Western blot - Anti-Flotillin 1 antibody [EP446Y] (AB78178), expandable thumbnail
  • Western blot - Anti-Flotillin 1 antibody [EP446Y] (AB78178), expandable thumbnail
  • Western blot - Anti-Flotillin 1 antibody [EP446Y] (AB78178), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

For unpurified, use 1/500 - 1/1000.

Species

Rat

Dilution info

1/2000

Notes

For unpurified, use 1/500 - 1/1000.

Species

Human

Dilution info

1/2000

Notes

For unpurified, use 1/500 - 1/1000.

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.

Alternative names

Flotillin-1, FLOT1

Recommended products

Rabbit Recombinant Monoclonal Flotillin 1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP446Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Flotillin 1 also known as flotillin-1 or flot-1 functions as a major scaffolding protein in lipid rafts which are specialized membrane microdomains. It has a molecular weight of approximately 47 kDa facilitating protein interactions within the cell membrane. Flotillin 1 localizes primarily to non-caveolar lipid rafts and is expressed widely across various tissue types. High expression levels occur in brain tissue contributing to its recognition as an important element within neuronal membranes.

Biological function summary

Flotillin-1 participates in vesicle trafficking and endocytosis by forming a complex with its counterpart flotillin-2. This complex stabilizes membrane microdomains and assists in signaling pathways. Flotillin-1 plays a significant role in cell adhesion migration and cytoskeleton organization by interacting with actin and other cytoskeletal proteins. By doing so it influences cellular processes related to membrane shaping and intracellular transport.

Pathways

Flotillin-1 integrates into the MAPK signaling pathway and the Wnt signaling pathway which are critical for cell differentiation and proliferation. It interacts with several proteins including caveolin-1 that have known roles in these pathways. These interactions help modulate signaling cascades providing necessary cellular responses to various extracellular stimuli.

Associated diseases and disorders

Flotillin-1 has associations with Alzheimer's disease and various cancers. In Alzheimer's disease it interacts with amyloid precursor protein influencing amyloid-beta production and deposition. In cancer altered expression levels of flotillin-1 affect tumor cell adhesion and migration with its interactions impacting the expression of proteins like E-cadherin which is associated with cancer metastasis and progression. Understanding these interactions offers potential insights for developing therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178), expandable thumbnail

    Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)

    Lanes 1-4: Merged signal (red and green). Green - ab78178 observed at 50 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab78178 Anti-Flotillin 1 antibody [EP446Y] was shown to specifically react with Flotillin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human FLOT1 (Flotillin 1) knockout HEK-293T cell line ab267276 (knockout cell lysate Human FLOT1 (Flotillin 1) knockout HEK-293T cell lysate ab257109) was used. Wild-type and Flotillin knockout samples were subjected to SDS-PAGE. ab78178 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: FLOT1 knockout HEK293T cell lysate at 20 µg

    Lane 3: A431 cell lysate at 20 µg

    Lane 4: HAP1 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 47 kDa

    Observed band size: 50 kDa

    This data was developed using the same antibody clone in a different buffer formulation (ab78178).

    Lanes 1-4: Merged signal (red and green). Green - ab78178 observed at 50 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab78178 Anti-Flotillin 1 antibody [EP446Y] was shown to specifically react with Flotillin 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human FLOT1 (Flotillin 1) knockout HEK-293T cell line ab267276 (knockout cell lysate Human FLOT1 (Flotillin 1) knockout HEK-293T cell lysate ab257109) was used. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. ab78178 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178), expandable thumbnail

    Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: Flotillin 1 knockout HAP1 whole cell lysate (20 μg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab78178 observed at 47 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab78178 was shown to specifically react with Flotillin 1 in wild-type HAP1 cells as signal was lost in Flotillin 1 knockout cells. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. ab78178 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)

    Predicted band size: 47 kDa

  • Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178), expandable thumbnail

    Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/2000 dilution

    Lane 1: mouse brain lysate at 10 µg

    Lane 2: rat brain lysate at 20 µg

    Lane 3: C6 cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 47 kDa

    Observed band size: 49 kDa

  • Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178), expandable thumbnail

    Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/2000 dilution

    Lane 1: K562 cell lysate at 20 µg

    Lane 2: A431 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 47 kDa

    Observed band size: 49 kDa

  • Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178), expandable thumbnail

    Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)

    All lanes: Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/500 dilution

    All lanes: K562 cell lysate

    Predicted band size: 47 kDa

    Observed band size: 48 kDa

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Product protocols

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