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AB247493

Anti-Flotillin 1 antibody [EP446Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal Flotillin 1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

Flotillin-1, FLOT1

1 Images
Western blot - Anti-Flotillin 1 antibody [EP446Y] - BSA and Azide free (AB247493)
  • WB

Unknown

Western blot - Anti-Flotillin 1 antibody [EP446Y] - BSA and Azide free (AB247493)

This data was developed using the same antibody clone in a different buffer formulation (ab78178).

Lanes 1-4 : Merged signal (red and green). Green - ab78178 observed at 50 kDa. Red - loading control ab8245 observed at 36 kDa.

ab78178 Anti-Flotillin 1 antibody [EP446Y] was shown to specifically react with Flotillin 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267276 (knockout cell lysate ab257109) was used. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. ab78178 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Flotillin 1 antibody [EP446Y] (<a href='/en-us/products/primary-antibodies/flotillin-1-antibody-ep446y-ab78178'>ab78178</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

FLOT1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human FLOT1 (Flotillin 1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-flot1-flotillin-1-knockout-hek-293t-cell-line-ab267276'>ab267276</a>)

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

HAP1 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 47 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP446Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Flotillin 1 also known as flotillin-1 or flot-1 functions as a major scaffolding protein in lipid rafts which are specialized membrane microdomains. It has a molecular weight of approximately 47 kDa facilitating protein interactions within the cell membrane. Flotillin 1 localizes primarily to non-caveolar lipid rafts and is expressed widely across various tissue types. High expression levels occur in brain tissue contributing to its recognition as an important element within neuronal membranes.
Biological function summary

Flotillin-1 participates in vesicle trafficking and endocytosis by forming a complex with its counterpart flotillin-2. This complex stabilizes membrane microdomains and assists in signaling pathways. Flotillin-1 plays a significant role in cell adhesion migration and cytoskeleton organization by interacting with actin and other cytoskeletal proteins. By doing so it influences cellular processes related to membrane shaping and intracellular transport.

Pathways

Flotillin-1 integrates into the MAPK signaling pathway and the Wnt signaling pathway which are critical for cell differentiation and proliferation. It interacts with several proteins including caveolin-1 that have known roles in these pathways. These interactions help modulate signaling cascades providing necessary cellular responses to various extracellular stimuli.

Flotillin-1 has associations with Alzheimer's disease and various cancers. In Alzheimer's disease it interacts with amyloid precursor protein influencing amyloid-beta production and deposition. In cancer altered expression levels of flotillin-1 affect tumor cell adhesion and migration with its interactions impacting the expression of proteins like E-cadherin which is associated with cancer metastasis and progression. Understanding these interactions offers potential insights for developing therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
See full target information FLOT1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB41927

Anti-Flotillin 1 antibody

primary-antibodies

flotillin-1-antibody-ab41927

4

(10 reviews)

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