Rabbit Recombinant Monoclonal Flotillin 1 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 60 publications.
Images
![Immunoprecipitation - Anti-Flotillin 1 antibody [EPR6041] (AB133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--immunoprecipitation-img8363.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Flotillin 1 antibody [EPR6041] (AB133497)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] (AB133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15550.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] (AB133497)")
![Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody [EPR6041] (AB133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--immunocytochemistry-immunofluorescence-img20949.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody [EPR6041] (AB133497)")
![Western blot - Anti-Flotillin 1 antibody [EPR6041] (AB133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--western-blot-img4948.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Flotillin 1 antibody [EPR6041] (AB133497)")
![Flow Cytometry (Intracellular) - Anti-Flotillin 1 antibody [EPR6041] (AB133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--flow-cytometry-intracellular-img37364.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Flotillin 1 antibody [EPR6041] (AB133497)")
Publications
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- International journal of molecular sciences 24:2023Comparison Study of Small Extracellular Vesicle Isolation Methods for Profiling Protein Biomarkers in Breast Cancer Liquid Biopsies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujin Lee,Jie Ni,Valerie C Wasinger,Peter Graham,Yong LiPubMed 37895140
- Journal of extracellular vesicles 12:e123652023Systematic compositional analysis of exosomal extracellular vesicles produced by cells undergoing apoptosis, necroptosis and ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin Cappe,Mike Vadi,Eliza Sack,Ludivine Wacheul,Bruno Verstraeten,Sara Dufour,Julien Franck,Wei Xie,Francis Impens,An Hendrix,Denis L J Lafontaine,Peter Vandenabeele,Franck B RiquetPubMed 37807017
- Nature communications 14:50342023Reactivated endogenous retroviruses promote protein aggregate spreading.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu Liu,Stefanie-Elisabeth Heumüller,André Hossinger,Stephan A Müller,Oleksandra Buravlova,Stefan F Lichtenthaler,Philip Denner,Ina M VorbergPubMed 37596282
- Frontiers in cellular neuroscience 17:11176972023Epileptiform activity influences theta-burst induced LTP in the adult hippocampus: a role for synaptic lipid raft disruption in early metaplasticity?Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosé D Carvalho-Rosa,Nádia C Rodrigues,Armando Silva-Cruz,Sandra H Vaz,Diana Cunha-ReisPubMed 37228704
- Journal of lipid research 64:1003672023Cellular cholesterol loss by DHCR24 knockdown leads to Aβ production by changing APP intracellular localization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Huang,Wenbin Zhang,Xiaorou Guo,Ying Zhang,Junfeng Wu,Hengbing ZuPubMed 37011864
- Theranostics 13:1470-14892023Xeno-free cultured mesenchymal stromal cells release extracellular vesicles with a "therapeutic" miRNA cargo ameliorating cartilage inflammation .Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Elisabetta F Palamà,Simona Coco,Georgina M Shaw,Daniele Reverberi,Maddalena Ghelardoni,Paola Ostano,Giovanna Chiorino,Laura Sercia,Luana Persano,Maria Cristina Gagliani,Katia Cortese,Dario Pisignano,Josephine Mary Murphy,Chiara GentiliPubMed 37056573
- iScience 26:1061512023IGFBP2 derived from PO-MSCs promote epithelial barrier destruction by activating FAK signaling in nasal polyps.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo You,Ting Zhang,Wei Zhang,Yinyin Pei,Danyi Huang,Yun Lei,Shaocong Zhang,Changyu Qiu,Jie Zhang,Zhifeng Gu,Lei Cheng,Jing ChenPubMed 36866245
- Immunity & ageing : I & A 20:62023Mitochondrial DNA and inflammatory proteins are higher in extracellular vesicles from frail individuals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnjali M Byappanahalli,Nicole Noren Hooten,Mya Vannoy,Nicolle A Mode,Ngozi Ezike,Alan B Zonderman,Michele K EvansPubMed 36710345
- Reproductive biology and endocrinology : RB&E 20:1612022Endoplasmic reticulum stress increases exosome biogenesis and packaging relevant to sperm maturation in response to oxidative stress in obese mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYangyang Li,Wenzhen Zhao,Rong Fu,Zhuoyao Ma,Yanqin Hu,Yue Liu,Zhide DingPubMed 36411474
- JCI insight 7:2022Cell-free DNA topology depends on its subcellular and cellular origins in cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEthan Z Malkin,Steven De Michino,Meghan Lambie,Rita Gill,Zhen Zhao,Ariana Rostami,Andrea Arruda,Mark D Minden,Scott V BratmanPubMed 36125881
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected | Expected |
| Rat | Expected | Expected | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
Species Rat | Dilution info 1/10000 - 1/50000 | Notes - |
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Alternative names
Flotillin-1, FLOT1
Recommended products
Rabbit Recombinant Monoclonal Flotillin 1 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 60 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR6041
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Flotillin 1 also known as flotillin-1 or flot-1 functions as a major scaffolding protein in lipid rafts which are specialized membrane microdomains. It has a molecular weight of approximately 47 kDa facilitating protein interactions within the cell membrane. Flotillin 1 localizes primarily to non-caveolar lipid rafts and is expressed widely across various tissue types. High expression levels occur in brain tissue contributing to its recognition as an important element within neuronal membranes.
- Biological function summary
Flotillin-1 participates in vesicle trafficking and endocytosis by forming a complex with its counterpart flotillin-2. This complex stabilizes membrane microdomains and assists in signaling pathways. Flotillin-1 plays a significant role in cell adhesion migration and cytoskeleton organization by interacting with actin and other cytoskeletal proteins. By doing so it influences cellular processes related to membrane shaping and intracellular transport.
- Pathways
Flotillin-1 integrates into the MAPK signaling pathway and the Wnt signaling pathway which are critical for cell differentiation and proliferation. It interacts with several proteins including caveolin-1 that have known roles in these pathways. These interactions help modulate signaling cascades providing necessary cellular responses to various extracellular stimuli.
- Associated diseases and disorders
Flotillin-1 has associations with Alzheimer's disease and various cancers. In Alzheimer's disease it interacts with amyloid precursor protein influencing amyloid-beta production and deposition. In cancer altered expression levels of flotillin-1 affect tumor cell adhesion and migration with its interactions impacting the expression of proteins like E-cadherin which is associated with cancer metastasis and progression. Understanding these interactions offers potential insights for developing therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Immunoprecipitation - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--immunoprecipitation-img8363.jpg "Immunoprecipitation - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
Immunoprecipitation - Anti-Flotillin 1 antibody [EPR6041] (ab133497)
ab133497 (purified) at 1/60 immunoprecipitating Flotillin 1 in 10 μg K562 (Lanes 1 and 2, observed at 48 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-Flotillin 1 antibody [EPR6041] (ab133497)
Predicted band size: 42 kDa, 47 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15550.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] (ab133497)
Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab133497 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--immunocytochemistry-immunofluorescence-img20949.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody [EPR6041] (ab133497)
Immunofluorescence staining of Jurkat cells with purified ab133497 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab133497 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.
![Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--western-blot-img4948.jpg "Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)
Lanes 1-3: Merged signal (red and green). Green - ab133497 observed at 50 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
ab133497 Anti-Flotillin 1 antibody [EPR6041] was shown to specifically react with Flotillin 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human FLOT1 (Flotillin 1) knockout HEK-293T cell line ab267276 (knockout cell lysate Human FLOT1 (Flotillin 1) knockout HEK-293T cell lysate ab257109) was used. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. ab133497 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: FLOT1 knockout HEK293T cell lysate at 20 µg
Lane 3: HAP1 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 50 kDa
This data was developed using the same antibody clone in a different buffer formulation (ab133497).
Lanes 1-3: Merged signal (red and green). Green - ab133497 observed at 50 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
ab133497 Anti-Flotillin 1 antibody [EPR6041] was shown to specifically react with Flotillin 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human FLOT1 (Flotillin 1) knockout HEK-293T cell line ab267276 (knockout cell lysate Human FLOT1 (Flotillin 1) knockout HEK-293T cell lysate ab257109) was used. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. ab133497 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
![Flow Cytometry (Intracellular) - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--flow-cytometry-intracellular-img37364.jpg "Flow Cytometry (Intracellular) - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
Flow Cytometry (Intracellular) - Anti-Flotillin 1 antibody [EPR6041] (ab133497)
Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling Flotillin 1 with purified ab133497 at 1/50 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr®488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120781.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] (ab133497)
Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Flotillin 1 using unpurified ab133497 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--western-blot-img57951.jpg "Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: Flotillin 1 knockout HAP1 whole cell lysate (20 μg)Lanes 1 - 2: Merged signal (red and green). Green - ab133497 observed at 47 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
ab133497 was shown to specifically react with Flotillin 1 in wild-type HAP1 cells as signal was lost in Flotillin 1 knockout cells. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. ab133497 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)
Predicted band size: 47 kDa
Observed band size: 62 kDa
![Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--western-blot-img109551.jpg "Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497) at 1/10000 dilution
Lane 1: K562 cell lysate at 20 µg
Lane 2: A431 cell lysate at 20 µg
Lane 3: Jurkat cell lysate at 20 µg
Lane 4: HeLa cell lysate at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 48 kDa
![Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--western-blot-img104382.jpg "Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497) at 1/10000 dilution
Lane 1: mouse brain lysate at 20 µg
Lane 2: mouse kidney lysate at 20 µg
Lane 3: rat kidney lysate at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit IgG (H+L)
Predicted band size: 47 kDa
Observed band size: 48 kDa
![Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--western-blot-img116862.jpg "Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)
All lanes: Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497) at 1/10000 dilution
Lane 1: K562 cell lysate at 10 µg
Lane 2: A431 cell lysate at 10 µg
Lane 3: Jurkat cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 47 kDa
Observed band size: 48 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120781.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
![Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--western-blot-img57951.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
![Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--western-blot-img109551.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
![Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--western-blot-img104382.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
![Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-ab133497--western-blot-img116862.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Flotillin 1 antibody [EPR6041] (ab133497)")
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