Rabbit Recombinant Monoclonal Flotillin 1 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Immunoprecipitation - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (AB232405), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-bsa-and-azide-free-ab232405--immunoprecipitation-img8363.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (AB232405)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (AB232405), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-bsa-and-azide-free-ab232405--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15550.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (AB232405)")
![Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (AB232405), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-bsa-and-azide-free-ab232405--immunocytochemistry-immunofluorescence-img20949.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (AB232405)")
![Western blot - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (AB232405), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-bsa-and-azide-free-ab232405--western-blot-img4948.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (AB232405)")
![Flow Cytometry (Intracellular) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (AB232405), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-bsa-and-azide-free-ab232405--flow-cytometry-intracellular-img37364.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (AB232405)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected | Expected |
| Rat | Expected | Expected | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
2 products for Alternative Version
Target data
Function
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Alternative names
Flotillin-1, FLOT1
Recommended products
Rabbit Recombinant Monoclonal Flotillin 1 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR6041
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab232405 is the carrier-free version of Anti-Flotillin 1 antibody [EPR6041] ab133497.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Flotillin 1 also known as flotillin-1 or flot-1 functions as a major scaffolding protein in lipid rafts which are specialized membrane microdomains. It has a molecular weight of approximately 47 kDa facilitating protein interactions within the cell membrane. Flotillin 1 localizes primarily to non-caveolar lipid rafts and is expressed widely across various tissue types. High expression levels occur in brain tissue contributing to its recognition as an important element within neuronal membranes.
- Biological function summary
Flotillin-1 participates in vesicle trafficking and endocytosis by forming a complex with its counterpart flotillin-2. This complex stabilizes membrane microdomains and assists in signaling pathways. Flotillin-1 plays a significant role in cell adhesion migration and cytoskeleton organization by interacting with actin and other cytoskeletal proteins. By doing so it influences cellular processes related to membrane shaping and intracellular transport.
- Pathways
Flotillin-1 integrates into the MAPK signaling pathway and the Wnt signaling pathway which are critical for cell differentiation and proliferation. It interacts with several proteins including caveolin-1 that have known roles in these pathways. These interactions help modulate signaling cascades providing necessary cellular responses to various extracellular stimuli.
- Associated diseases and disorders
Flotillin-1 has associations with Alzheimer's disease and various cancers. In Alzheimer's disease it interacts with amyloid precursor protein influencing amyloid-beta production and deposition. In cancer altered expression levels of flotillin-1 affect tumor cell adhesion and migration with its interactions impacting the expression of proteins like E-cadherin which is associated with cancer metastasis and progression. Understanding these interactions offers potential insights for developing therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunoprecipitation - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-bsa-and-azide-free-ab232405--immunoprecipitation-img8363.jpg "Immunoprecipitation - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)")
Immunoprecipitation - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)
Anti-Flotillin 1 antibody [EPR6041] ab133497 (purified) at 1/60 immunoprecipitating Flotillin 1 in 10 µg K562 (Lanes 1 and 2, observed at 48 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBSTThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Flotillin 1 antibody [EPR6041] ab133497).
All lanes: Immunoprecipitation - Anti-Flotillin 1 antibody [EPR6041] (Anti-Flotillin 1 antibody [EPR6041] ab133497)
Predicted band size: 42 kDa, 47 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-bsa-and-azide-free-ab232405--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15550.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)
Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified Anti-Flotillin 1 antibody [EPR6041] ab133497 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Flotillin 1 antibody [EPR6041] ab133497).
![Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-bsa-and-azide-free-ab232405--immunocytochemistry-immunofluorescence-img20949.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)")
Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)
Immunofluorescence staining of Jurkat cells with purified Anti-Flotillin 1 antibody [EPR6041] ab133497 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified Anti-Flotillin 1 antibody [EPR6041] ab133497 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Flotillin 1 antibody [EPR6041] ab133497).
![Western blot - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-bsa-and-azide-free-ab232405--western-blot-img4948.jpg "Western blot - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)")
Western blot - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Flotillin 1 antibody [EPR6041] ab133497).
Lanes 1-3: Merged signal (red and green). Green - Anti-Flotillin 1 antibody [EPR6041] ab133497 observed at 50 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-Flotillin 1 antibody [EPR6041] ab133497 Anti-Flotillin 1 antibody [EPR6041] was shown to specifically react with Flotillin 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human FLOT1 (Flotillin 1) knockout HEK-293T cell line ab267276 (knockout cell lysate Human FLOT1 (Flotillin 1) knockout HEK-293T cell lysate ab257109) was used. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. Anti-Flotillin 1 antibody [EPR6041] ab133497 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Flotillin 1 antibody [EPR6041] (Anti-Flotillin 1 antibody [EPR6041] ab133497) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: FLOT1 knockout HEK293T cell lysate at 20 µg
Lane 3: HAP1 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 50 kDa
![Flow Cytometry (Intracellular) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-bsa-and-azide-free-ab232405--flow-cytometry-intracellular-img37364.jpg "Flow Cytometry (Intracellular) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)")
Flow Cytometry (Intracellular) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)
Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling Flotillin 1 with purified Anti-Flotillin 1 antibody [EPR6041] ab133497 at 1/50 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Flotillin 1 antibody [EPR6041] ab133497).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-bsa-and-azide-free-ab232405--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120781.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)
Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Flotillin 1 using unpurified Anti-Flotillin 1 antibody [EPR6041] ab133497 at a dilution of 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Flotillin 1 antibody [EPR6041] ab133497).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-bsa-and-azide-free-ab232405--western-blot-img57951.jpg "Western blot - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)")
Western blot - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: Flotillin 1 knockout HAP1 whole cell lysate (20 μg)Lanes 1 - 2: Merged signal (red and green). Green - Anti-Flotillin 1 antibody [EPR6041] ab133497 observed at 47 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-Flotillin 1 antibody [EPR6041] ab133497 was shown to specifically react with Flotillin 1 in wild-type HAP1 cells as signal was lost in Flotillin 1 knockout cells. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. Anti-Flotillin 1 antibody [EPR6041] ab133497 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Flotillin 1 antibody [EPR6041] ab133497).
All lanes: Western blot - Anti-Flotillin 1 antibody [EPR6041] (Anti-Flotillin 1 antibody [EPR6041] ab133497)
Predicted band size: 47 kDa
Observed band size: 62 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-bsa-and-azide-free-ab232405--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120781.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)")
![Western blot - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405), expandable thumbnail](https://content.abcam.com/products/images/flotillin-1-antibody-epr6041-bsa-and-azide-free-ab232405--western-blot-img57951.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide free (ab232405)")
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