AB113661

Anti-Flotillin 2/ESA antibody

Name: Anti-Flotillin 2/ESA antibody (ab113661) | Abcam
Brand: Abcam Limited
SKU: AB113661
Price: 645 USD
Availability: InStock

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(4 Publications)

Goat Polyclonal Flotillin 2/ESA antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human FLOT2 aa 400 to C-terminus.

View Alternative Names

ESA1, M17S1, FLOT2, Flotillin-2, Epidermal surface antigen, Membrane component chromosome 17 surface marker 1, ESA

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (AB113661)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (AB113661)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human Kidney with ab113661 at 3.75ug/ml

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (AB113661)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human Tonsil with ab113661 at 3.75ug/ml

Unknown

Western blot - Anti-Flotillin 2/ESA antibody (AB113661)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-Flotillin 2/ESA antibody (ab113661) at 0.1 µg/mL

All lanes:

Human Brain (Cerebellum) lysate at 35 µg/mL

Predicted band size: 47 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human FLOT2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14254

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Flotillin 2 also known as flotillin-2 or ESA is a protein weighing approximately 48 kDa and is a part of the flotillin family. It is expressed in various human tissues with high presence in the brain heart and skeletal muscles. This protein is an integral component of the lipid raft microdomains in cell membranes where it assists in cellular processes such as signal transduction and lipid trafficking. Its membrane association depends on N-terminal hydrophobic sequences which contribute to its distribution on the cell surface.

Biological function summary

Flotillins serve as scaffolding proteins in cellular signaling and play a part in the regulation of endocytosis. Flotillin 2 often forms a hetero-oligomeric complex with Flotillin 1 facilitating its role in organizing membrane microdomains. This interaction supports cellular activities like molecular transport and signal relay. Flotillin 2 contributes to dynamic cellular structures and has been suggested to influence actin cytoskeleton assembly giving it a role in cellular morphology and motility.

Pathways

Flotillin 2 associates with important cell signaling pathways notably the MAPK and Wnt signaling pathways. In MAPK pathways flotillin 2 influences cell proliferation and differentiation by modulating interaction with signaling proteins like Ras and ERK. In the context of the Wnt signaling pathway it impacts cellular communication influencing gene expression and development processes by engaging with Dishevelled proteins. These pathways highlight its critical part in processes like cellular growth and communication.

Flotillin 2 exhibits connections regarding metabolic and neurodegenerative conditions. Flotillin 2 upregulation appears linked with metabolic syndromes associating with insulin signaling disruptions. Furthermore alterations in its expression relate to neurodegenerative diseases like Alzheimer's where it impacts amyloid precursor protein processing and tau protein pathology. These associations position flotillin 2 as a significant player in the understanding and potential treatment of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.

See full target information FLOT2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 151: PubMed39576204

2024

WNT7A-positive dendritic cytonemes control synaptogenesis in cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas M Piers,Kevin Fang,Seema C Namboori,Corin Liddle,Sally Rogers,Akshay Bhinge,Richard Killick,Steffen Scholpp

Heliyon 9:e18082 PubMed37539272

2023

Loss of Flot2 expression in deep cerebellar nuclei neurons of mice with Niemann-Pick disease type C.

Applications

Unspecified application

Species

Unspecified reactive species

Tsu-I Chen,Pei-Chun Hsu,Ni-Chung Lee,Yu-Han Liu,Hao-Chun Wang,Yen-Hsu Lu,Yin-Hsiu Chien,Wuh-Liang Hwu

eLife 11: PubMed36040316

2022

The scaffolding protein flot2 promotes cytoneme-based transport of wnt3 in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Routledge,Sally Rogers,Yosuke Ono,Lucy Brunt,Valerie Meniel,Giusy Tornillo,Hassan Ashktorab,Toby J Phesse,Steffen Scholpp

Development (Cambridge, England) 144:321-333 PubMed27993984

2016

An Epha4/Sipa1l3/Wnt pathway regulates eye development and lens maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie Rothe,Noreen Kanwal,Petra Dietmann,Franziska A Seigfried,Annemarie Hempel,Desiree Schütz,Dominik Reim,Rebecca Engels,Alexander Linnemann,Michael J Schmeisser,Juergen Bockmann,Michael Kühl,Tobias M Boeckers,Susanne J Kühl

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Flotillin 2/ESA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (AB113661)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (AB113661)

Western blot - Anti-Flotillin 2/ESA antibody (AB113661)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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