AB96507

Anti-Flotillin 2/ESA antibody

Name: Anti-Flotillin 2/ESA antibody (ab96507) | Abcam
Brand: Abcam Limited
SKU: AB96507
Price: 535 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(11 Publications)

Rabbit Polyclonal Flotillin 2/ESA antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human FLOT2 aa 350 to C-terminus.

View Alternative Names

ESA1, M17S1, FLOT2, Flotillin-2, Epidermal surface antigen, Membrane component chromosome 17 surface marker 1, ESA

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 2/ESA antibody (AB96507)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 2/ESA antibody (AB96507)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa S3 cells, using ab96507 at 1 : 100 dilution. Image 2 : Merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (AB96507)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (AB96507)

Immunohistochemical analysis of paraffin-embedded human lung cancer using ab96507 at 1 : 100 dilution. Antigen Retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Supplier Data

Immunoprecipitation - Anti-Flotillin 2/ESA antibody (AB96507)

Flotillin 2 was immunoprecipitated from 5μ of HeLa whole cell lysate with ab96507. Western blot was performed from the immunoprecipitate using ab96507. EasyBlot anti-Rabbit IgG, was used as secondary antibody.

Lane 1 : HeLa lysate 5μg (Input).
Lane 2 : ab96507 IP in HeLa lysate.

All lanes:

Immunoprecipitation - Anti-Flotillin 2/ESA antibody (ab96507)

Predicted band size: 47 kDa

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AbReview34046****

Immunoprecipitation - Anti-Flotillin 2/ESA antibody (AB96507)

Flotillin 2/ESA immunoprecipitation from Human HeLa whole cell lysate using ab96507. 750 μg of cell lysate was incubated with primary antibody (1/500) and matrix (Protein A) and incubated for 16 hours at 4°C.

Lane 1 : anti-Flotillin 2/ESA IP (using non-Abcam antibody)
Lane 2 : anti-Flotillin 2/ESA IP (using ab96507)
Lane 3 : Input (HeLa cell lysate)
Lane 4 : Supernatant IP (using non-Abcam antibody)
Lane 5 : Supernatant IP (using ab96507)

Western blot detection : mouse anti-Flotillin 2/ESA antibody + HRP donkey anti-mouse polyclonal secondary antibody

All lanes:

Immunoprecipitation - Anti-Flotillin 2/ESA antibody (ab96507)

Predicted band size: 47 kDa

false

This image is courtesy of an Abreview submitted by Prof Ritva Tikkanen

Unknown

Western blot - Anti-Flotillin 2/ESA antibody (AB96507)

10% SDS PAGE

All lanes:

Western blot - Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution

All lanes:

HeLa S3 whole cell lysate at 30 µg

Predicted band size: 47 kDa

false

Supplier Data

Western blot - Anti-Flotillin 2/ESA antibody (AB96507)

10% SDS-PAGE

All lanes:

Western blot - Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution

All lanes:

Rat brain lysate at 50 µg

Predicted band size: 47 kDa

false

Supplier Data

Western blot - Anti-Flotillin 2/ESA antibody (AB96507)

All lanes:

Western blot - Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution

All lanes:

Mouse brain lysate at 50 µg

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, IP, WB

applications

Immunogen

Synthetic Peptide within Human FLOT2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14254

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Antigen retrieval is not essential but may optimise staining.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Flotillin 2 also known as flotillin-2 or ESA is a protein weighing approximately 48 kDa and is a part of the flotillin family. It is expressed in various human tissues with high presence in the brain heart and skeletal muscles. This protein is an integral component of the lipid raft microdomains in cell membranes where it assists in cellular processes such as signal transduction and lipid trafficking. Its membrane association depends on N-terminal hydrophobic sequences which contribute to its distribution on the cell surface.

Biological function summary

Flotillins serve as scaffolding proteins in cellular signaling and play a part in the regulation of endocytosis. Flotillin 2 often forms a hetero-oligomeric complex with Flotillin 1 facilitating its role in organizing membrane microdomains. This interaction supports cellular activities like molecular transport and signal relay. Flotillin 2 contributes to dynamic cellular structures and has been suggested to influence actin cytoskeleton assembly giving it a role in cellular morphology and motility.

Pathways

Flotillin 2 associates with important cell signaling pathways notably the MAPK and Wnt signaling pathways. In MAPK pathways flotillin 2 influences cell proliferation and differentiation by modulating interaction with signaling proteins like Ras and ERK. In the context of the Wnt signaling pathway it impacts cellular communication influencing gene expression and development processes by engaging with Dishevelled proteins. These pathways highlight its critical part in processes like cellular growth and communication.

Flotillin 2 exhibits connections regarding metabolic and neurodegenerative conditions. Flotillin 2 upregulation appears linked with metabolic syndromes associating with insulin signaling disruptions. Furthermore alterations in its expression relate to neurodegenerative diseases like Alzheimer's where it impacts amyloid precursor protein processing and tau protein pathology. These associations position flotillin 2 as a significant player in the understanding and potential treatment of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.

See full target information FLOT2

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

PNAS nexus 3:pgae496 PubMed39660059

2024

Choroid plexus extracellular vesicle transport of blood-borne insulin-like growth factor 1 to the hippocampus of the immature brain.

Applications

Unspecified application

Species

Unspecified reactive species

Niklas Ortenlöf,Suvi Vallius,Helena Karlsson,Claes Ekström,Amanda Kristiansson,Bo Holmqvist,Stanislava Pankratova,Norman Barton,David Ley,Magnus Gram

Journal of extracellular vesicles 13:e70011 PubMed39508423

2024

Efficient enzyme-free isolation of brain-derived extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Andreu Matamoros-Angles,Emina Karadjuzovic,Behnam Mohammadi,Feizhi Song,Santra Brenna,Susanne Caroline Meister,Bente Siebels,Hannah Voß,Carolin Seuring,Isidre Ferrer,Hartmut Schlüter,Matthias Kneussel,Hermann Clemens Altmeppen,Michaela Schweizer,Berta Puig,Mohsin Shafiq,Markus Glatzel

CNS neuroscience & therapeutics 30:e70065 PubMed39350328

2024

Comprehensive Proteomic Analysis of Dysferlinopathy Unveiling Molecular Mechanisms and Biomarkers Linked to Pathological Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wang,Xin-Yi Liu,Qi-Fang He,Fu-Ze Zheng,Long Chen,Ying Zheng,Ming-Hui Zeng,Yu-Hua Lin,Xin Lin,Hai-Zhu Chen,Min-Ting Lin,Ning Wang,Zhi-Qiang Wang,Feng Lin

Balkan medical journal 39:267-274 PubMed35872627

2022

FLOT2 Promotes the Proliferation and Epithelial-mesenchymal Transition of Cervical Cancer by Activating the MEK/ERK1/2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Shulan Dou

Cells 11: PubMed35159121

2022

MPP1 Determines the Mobility of Flotillins and Controls the Confinement of Raft-Associated Molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka Biernatowska,Karolina Wójtowicz,Tomasz Trombik,Aleksander F Sikorski,Aleksander Czogalla

The Journal of cell biology 220: PubMed34309628

2021

Extracellular vesicles from neurons promote neural induction of stem cells through cyclin D1.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Song,Xinran Tian,Randy Schekman

Cryobiology 87:32-39 PubMed30876909

2019

Cryopreservation disrupts lipid rafts and heat shock proteins in yellow catfish sperm.

Applications

Unspecified application

Species

Unspecified reactive species

Chenglian Bai,Ning Kang,Junping Zhao,Jun Dai,Hui Gao,Yuanhong Chen,Haojia Dong,Changjiang Huang,Qiaoxiang Dong

Oncology letters 17:2802-2808 PubMed30854055

2019

FLOT2 overexpression is associated with the progression and prognosis of human colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Taiyuan Li,Chuanlin Cao,Qiangqiang Xiong,Dongning Liu

The Journal of cell biology 214:35-44 PubMed27354374

2016

Exosomes mediate cell contact-independent ephrin-Eph signaling during axon guidance.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Gong,Roman Körner,Louise Gaitanos,Rüdiger Klein

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 37:3349-57 PubMed26443540

2015

Overexpression of acylglycerol kinase is associated with poorer prognosis and lymph node metastasis in nasopharyngeal carcinoma.

Applications

IHC, WB

Species

Unspecified reactive species, Unspecified reactive species

Qian Zhu,Su-Mei Cao,Huan-Xin Lin,Qi Yang,Sai-Lan Liu,Ling Guo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Flotillin 2/ESA antibody

Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 2/ESA antibody (AB96507)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (AB96507)

Immunoprecipitation - Anti-Flotillin 2/ESA antibody (AB96507)

Immunoprecipitation - Anti-Flotillin 2/ESA antibody (AB96507)

Western blot - Anti-Flotillin 2/ESA antibody (AB96507)

Western blot - Anti-Flotillin 2/ESA antibody (AB96507)

Western blot - Anti-Flotillin 2/ESA antibody (AB96507)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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