Rabbit Recombinant Monoclonal Flotillin 2/ESA antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Flotillin 2/ESA antibody [EPR26975-46] - BSA and Azide free (Capture) (AB314465), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-46-bsa-and-azide-free-capture-ab314465--sandwich-elisa-img423225.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Flotillin 2/ESA antibody [EPR26975-46] - BSA and Azide free (Capture) (AB314465)")
![Sandwich ELISA - Anti-Flotillin 2/ESA antibody [EPR26975-46] - BSA and Azide free (Capture) (AB314465), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-46-bsa-and-azide-free-capture-ab314465--sandwich-elisa-img423224.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Flotillin 2/ESA antibody [EPR26975-46] - BSA and Azide free (Capture) (AB314465)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
Alternative names
ESA1, M17S1, FLOT2, Flotillin-2, Epidermal surface antigen, Membrane component chromosome 17 surface marker 1, ESA
Recommended products
Rabbit Recombinant Monoclonal Flotillin 2/ESA antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26975-46
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab314465 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Flotillin 2/ESA. The recommended pair for sandwich ELISA is:
Capture: ab314465, Human Flotillin 2/ESA Capture Antibody (unconjugated)
Detector: Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (Detector) ab314466, Human Flotillin 2/ESA Detector Antibody (unconjugated)
The reference range value is 0.31-20 ng/mL.
The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Flotillin 2 also known as flotillin-2 or ESA is a protein weighing approximately 48 kDa and is a part of the flotillin family. It is expressed in various human tissues with high presence in the brain heart and skeletal muscles. This protein is an integral component of the lipid raft microdomains in cell membranes where it assists in cellular processes such as signal transduction and lipid trafficking. Its membrane association depends on N-terminal hydrophobic sequences which contribute to its distribution on the cell surface.
- Biological function summary
Flotillins serve as scaffolding proteins in cellular signaling and play a part in the regulation of endocytosis. Flotillin 2 often forms a hetero-oligomeric complex with Flotillin 1 facilitating its role in organizing membrane microdomains. This interaction supports cellular activities like molecular transport and signal relay. Flotillin 2 contributes to dynamic cellular structures and has been suggested to influence actin cytoskeleton assembly giving it a role in cellular morphology and motility.
- Pathways
Flotillin 2 associates with important cell signaling pathways notably the MAPK and Wnt signaling pathways. In MAPK pathways flotillin 2 influences cell proliferation and differentiation by modulating interaction with signaling proteins like Ras and ERK. In the context of the Wnt signaling pathway it impacts cellular communication influencing gene expression and development processes by engaging with Dishevelled proteins. These pathways highlight its critical part in processes like cellular growth and communication.
- Associated diseases and disorders
Flotillin 2 exhibits connections regarding metabolic and neurodegenerative conditions. Flotillin 2 upregulation appears linked with metabolic syndromes associating with insulin signaling disruptions. Furthermore alterations in its expression relate to neurodegenerative diseases like Alzheimer's where it impacts amyloid precursor protein processing and tau protein pathology. These associations position flotillin 2 as a significant player in the understanding and potential treatment of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Sandwich ELISA - Anti-Flotillin 2/ESA antibody [EPR26975-46] - BSA and Azide free (Capture) (ab314465), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-46-bsa-and-azide-free-capture-ab314465--sandwich-elisa-img423225.jpg "Sandwich ELISA - Anti-Flotillin 2/ESA antibody [EPR26975-46] - BSA and Azide free (Capture) (ab314465)")
Sandwich ELISA - Anti-Flotillin 2/ESA antibody [EPR26975-46] - BSA and Azide free (Capture) (ab314465)
Sandwich ELISA of Human Flotillin 2/ESA Antibody Pair - BSA and Azide free ab314464 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL. Interpolated concentrations of native Flotillin 2/ESA in human control wild type HEK293T cell and FLOT2 (Flotillin 2/ESA) knockout HEK293T cell based on 500 µg/mL extract loads. The concentrations of Flotillin 2/ESA were measured in duplicate and interpolated from the Flotillin 2/ESA standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Flotillin 2/ESA concentration was determined to be 1695.4 pg/mL in wild type HEK293T extract (Human wild-type HEK293T cell line ab255449) and undetectable in FLOT2 (Flotillin 2/ESA) knockout HEK293T extract (Human FLOT2 (Flotillin 2/ESA) knockout HEK293T cell line Human FLOT2 (Flotillin 2/ESA) knockout HEK-293T cell line ab266847).
![Sandwich ELISA - Anti-Flotillin 2/ESA antibody [EPR26975-46] - BSA and Azide free (Capture) (ab314465), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-46-bsa-and-azide-free-capture-ab314465--sandwich-elisa-img423224.jpg "Sandwich ELISA - Anti-Flotillin 2/ESA antibody [EPR26975-46] - BSA and Azide free (Capture) (ab314465)")
Sandwich ELISA - Anti-Flotillin 2/ESA antibody [EPR26975-46] - BSA and Azide free (Capture) (ab314465)
Sandwich ELISA of Human Flotillin 2/ESA Antibody Pair - BSA and Azide free ab314464 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
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