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Rabbit Recombinant Monoclonal Flotillin 2/ESA antibody. Suitable for IP, Flow Cyt, IHC-P, WB and reacts with Human samples.

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Images

Immunoprecipitation - Anti-Flotillin 2/ESA antibody [EPR26975-52] (AB307422), expandable thumbnail
  • Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] (AB307422), expandable thumbnail
  • Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] (AB307422), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] (AB307422), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] (AB307422), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytIHC-PWB
Human
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.

Alternative names

Flotillin-2, Epidermal surface antigen, Membrane component chromosome 17 surface marker 1, ESA, FLOT2, ESA1, M17S1

Recommended products

Rabbit Recombinant Monoclonal Flotillin 2/ESA antibody. Suitable for IP, Flow Cyt, IHC-P, WB and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR26975-52

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Flotillin 2 also known as flotillin-2 or ESA is a protein weighing approximately 48 kDa and is a part of the flotillin family. It is expressed in various human tissues with high presence in the brain heart and skeletal muscles. This protein is an integral component of the lipid raft microdomains in cell membranes where it assists in cellular processes such as signal transduction and lipid trafficking. Its membrane association depends on N-terminal hydrophobic sequences which contribute to its distribution on the cell surface.

Biological function summary

Flotillins serve as scaffolding proteins in cellular signaling and play a part in the regulation of endocytosis. Flotillin 2 often forms a hetero-oligomeric complex with Flotillin 1 facilitating its role in organizing membrane microdomains. This interaction supports cellular activities like molecular transport and signal relay. Flotillin 2 contributes to dynamic cellular structures and has been suggested to influence actin cytoskeleton assembly giving it a role in cellular morphology and motility.

Pathways

Flotillin 2 associates with important cell signaling pathways notably the MAPK and Wnt signaling pathways. In MAPK pathways flotillin 2 influences cell proliferation and differentiation by modulating interaction with signaling proteins like Ras and ERK. In the context of the Wnt signaling pathway it impacts cellular communication influencing gene expression and development processes by engaging with Dishevelled proteins. These pathways highlight its critical part in processes like cellular growth and communication.

Associated diseases and disorders

Flotillin 2 exhibits connections regarding metabolic and neurodegenerative conditions. Flotillin 2 upregulation appears linked with metabolic syndromes associating with insulin signaling disruptions. Furthermore alterations in its expression relate to neurodegenerative diseases like Alzheimer's where it impacts amyloid precursor protein processing and tau protein pathology. These associations position flotillin 2 as a significant player in the understanding and potential treatment of these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunoprecipitation - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422), expandable thumbnail

    Immunoprecipitation - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422)

    Flotillin 2/ESA was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug with ab307422 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307422 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug
    Lane 2: ABAB307422 IP in HEK-293T whole cell lysate
    Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307422 in HEK-293T whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 24 seconds

    All lanes: Immunoprecipitation - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422) at 1/1000 dilution

    Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 μg

    Lane 2: HEK-293T whole cell lysate

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 47 kDa

    Exposure time: 24s

  • Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422), expandable thumbnail

    Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422)

    Blocking and diluting buffer and concentration: Intercept? (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBSThe samples were run on a Bis-Tris gel.

    Performed under reducing conditions.

    False colour image of Western blot: Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.
    In Western blot, ab307422 was shown to bind specifically to Flotillin 2/ESA. A band was observed at 47 kDa in wild-type HEK-293T cell lysates whereas no signal observed at this size in Flotillin 2/ESA knockout cell line Human FLOT2 (Flotillin 2/ESA) knockout HEK-293T cell line ab266847 (knockout cell lysate Human FLOT2 (Flotillin 2/ESA) knockout HEK-293T cell lysate ab257442). . To generate this image, wild-type and Flotillin 2/ESA knockout HEK-293T cell lysates were analyzed. First, samples were run on a SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in in Intercept? (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4 ЎгC. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye? 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution and Goat anti-Mouse IgG H&L (IRDye? 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
    Exposure time:

    All lanes: Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg

    Lane 2: Flotillin 2/ESA knockout HEK-293T whole cell lysate 20 μg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 μg

    Secondary

    Lanes 1 - 3: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Lanes 1, 2 and 4: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 47 kDa

  • Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422), expandable thumbnail

    Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Exposure time:

    All lanes: Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422) at 1/1000 dilution

    Lane 1: Human breast tissue lysate 20 μg

    Lane 2: Human skin tissue lysate 20 μg

    Lane 3: Human lung tissue lysate 20 μg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 47 kDa

    Exposure time: 114s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue lABeling Flotillin 2/ESA with ab307422 at 1/1000 (0.535 ug/ml) followed by a ready to use RABbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human tonsil. The section was incubated with ab307422 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use RABbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular tissue lABeling Flotillin 2/ESA with ab307422 at 1/1000 (0.535 ug/ml) followed by a ready to use RABbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human hepatocellular carcinoma (PMID: 28560058). The section was incubated with ab307422 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use RABbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422)

    Immunohistochemical analysis of paraffin-embedded Human breast carcino tissue lABeling Flotillin 2/ESA with ab307422 at 1/1000 (0.535 ug/ml) followed by a ready to use RABbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human breast carcinoma (PMID: 22869152). The section was incubated with ab307422 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use RABbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422)

    Immunohistochemical analysis of paraffin-embedded (A) Wild-type HEK293 tissue lABeling Flotillin 2/ESA with ab307422 at 1/1000 (0.535 ug/ml) followed by a ready to use RABbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on (A) wild-type HEK293T cell pellet, no staining on (B) FLOT2 knockout HEK293T cell pellet. The section was incubated with ab307422 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use RABbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Flow Cytometry (Intracellular) - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Flotillin 2/ESA antibody [EPR26975-52] (ab307422)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized Wild-type 293T (human embryonic kidney epithelial cell, Right) / Flotillin 2/ESA knockout 293T (Left) cells lABelling Flotillin 2/ESA with ab307422 at 1/50 dilution (1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Positive staining on 293T cells (Human wild-type HEK-293T cell line ab255449), while no staining on Flotillin 2/ESA knockout 293T cells (Human FLOT2 (Flotillin 2/ESA) knockout HEK-293T cell line ab266847).

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Product protocols

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