Rabbit Recombinant Monoclonal Flotillin 2/ESA antibody. Carrier free. Suitable for IP, Flow Cyt, IHC-P, WB and reacts with Human samples.
Images
![Immunoprecipitation - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (AB307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--immunoprecipitation-img406400.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (AB307423)")
![Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (AB307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--western-blot-img405527.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (AB307423)")
![Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (AB307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--western-blot-img405526.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (AB307423)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (AB307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405524.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (AB307423)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (AB307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405523.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (AB307423)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | IHC-P | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Target data
Function
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
Alternative names
Flotillin-2, Epidermal surface antigen, Membrane component chromosome 17 surface marker 1, ESA, FLOT2, ESA1, M17S1
Recommended products
Rabbit Recombinant Monoclonal Flotillin 2/ESA antibody. Carrier free. Suitable for IP, Flow Cyt, IHC-P, WB and reacts with Human samples.
Alternative names
Flotillin-2, Epidermal surface antigen, Membrane component chromosome 17 surface marker 1, ESA, FLOT2, ESA1, M17S1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR26975-52
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Flotillin 2 also known as flotillin-2 or ESA is a protein weighing approximately 48 kDa and is a part of the flotillin family. It is expressed in various human tissues with high presence in the brain heart and skeletal muscles. This protein is an integral component of the lipid raft microdomains in cell membranes where it assists in cellular processes such as signal transduction and lipid trafficking. Its membrane association depends on N-terminal hydrophobic sequences which contribute to its distribution on the cell surface.
- Biological function summary
Flotillins serve as scaffolding proteins in cellular signaling and play a part in the regulation of endocytosis. Flotillin 2 often forms a hetero-oligomeric complex with Flotillin 1 facilitating its role in organizing membrane microdomains. This interaction supports cellular activities like molecular transport and signal relay. Flotillin 2 contributes to dynamic cellular structures and has been suggested to influence actin cytoskeleton assembly giving it a role in cellular morphology and motility.
- Pathways
Flotillin 2 associates with important cell signaling pathways notably the MAPK and Wnt signaling pathways. In MAPK pathways flotillin 2 influences cell proliferation and differentiation by modulating interaction with signaling proteins like Ras and ERK. In the context of the Wnt signaling pathway it impacts cellular communication influencing gene expression and development processes by engaging with Dishevelled proteins. These pathways highlight its critical part in processes like cellular growth and communication.
- Associated diseases and disorders
Flotillin 2 exhibits connections regarding metabolic and neurodegenerative conditions. Flotillin 2 upregulation appears linked with metabolic syndromes associating with insulin signaling disruptions. Furthermore alterations in its expression relate to neurodegenerative diseases like Alzheimer's where it impacts amyloid precursor protein processing and tau protein pathology. These associations position flotillin 2 as a significant player in the understanding and potential treatment of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunoprecipitation - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--immunoprecipitation-img406400.jpg "Immunoprecipitation - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)")
Immunoprecipitation - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)
This data was developed using Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422, the same antibody clone in a different buffer formulation.
Flotillin 2/ESA was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug with Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug
Lane 2: ABAB307422 IP in HEK-293T whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 in HEK-293T whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 secondsAll lanes: Immunoprecipitation - Anti-Flotillin 2/ESA antibody [EPR26975-52] (Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 μg
Lane 2: HEK-293T whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 47 kDa
Exposure time: 24s
Flotillin 2/ESA was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug with Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug
Lane 2: ABAB307422 IP in HEK-293T whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 in HEK-293T whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds![Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--western-blot-img405527.jpg "Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)")
Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)
This data was developed using 307422, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: The samples were run on a Bis-Tris gel.
Performed under reducing conditions.
False colour image of Western blot: Anti-Flotillin 2/ESA antibody [EPR26975-52] (Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.
In Western blot, Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 was shown to bind specifically to Flotillin 2/ESA. A band was observed at 47 kDa in wild-type HEK-293T cell lysates whereas no signal observed at this size in Flotillin 2/ESA knockout cell line Human FLOT2 (Flotillin 2/ESA) knockout HEK-293T cell line ab266847 (knockout cell lysate Human FLOT2 (Flotillin 2/ESA) knockout HEK-293T cell lysate ab257442). . To generate this image, wild-type and Flotillin 2/ESA knockout HEK-293T cell lysates were analyzed. First, samples were run on a SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in in Intercept? (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4 ЎгC. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye? 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution and Goat anti-Mouse IgG H&L (IRDye? 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
Exposure time:All lanes: Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] (Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422) at 1/1000 dilution
Lane 1: Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg
Lane 2: Flotillin 2/ESA knockout HEK-293T whole cell lysate 20 μg
Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 μg
SecondaryLanes 1 - 3: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Lanes 1, 2 and 4: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 47 kDa
Blocking and diluting buffer and concentration: Intercept? (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBSThe samples were run on a Bis-Tris gel.
Performed under reducing conditions.
False colour image of Western blot: Anti-Flotillin 2/ESA antibody [EPR26975-52] (Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.
In Western blot, Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 was shown to bind specifically to Flotillin 2/ESA. A band was observed at 47 kDa in wild-type HEK-293T cell lysates whereas no signal observed at this size in Flotillin 2/ESA knockout cell line Human FLOT2 (Flotillin 2/ESA) knockout HEK-293T cell line ab266847 (knockout cell lysate Human FLOT2 (Flotillin 2/ESA) knockout HEK-293T cell lysate ab257442). . To generate this image, wild-type and Flotillin 2/ESA knockout HEK-293T cell lysates were analyzed. First, samples were run on a SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in in Intercept? (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4 ЎгC. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye? 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution and Goat anti-Mouse IgG H&L (IRDye? 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
Exposure time:![Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--western-blot-img405526.jpg "Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)")
Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)
This data was developed using 307422, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration:
Exposure time:All lanes: Western blot - Anti-Flotillin 2/ESA antibody [EPR26975-52] (Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422) at 1/1000 dilution
Lane 1: Human breast tissue lysate 20 μg
Lane 2: Human skin tissue lysate 20 μg
Lane 3: Human lung tissue lysate 20 μg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 47 kDa
Exposure time: 114s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405524.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)
This data was developed using Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Flotillin 2/ESA with Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 at 1/1000 (0.535 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human tonsil. The section was incubated with Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405523.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)
This data was developed using Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular tissue labeling Flotillin 2/ESA with Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 at 1/1000 (0.535 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human hepatocellular carcinoma (PMID: 28560058). The section was incubated with Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405522.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)
This data was developed using Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human breast carcino tissue labeling Flotillin 2/ESA with Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 at 1/1000 (0.535 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human breast carcinoma (PMID: 22869152). The section was incubated with Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405521.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)
This data was developed using Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Wild-type HEK293 tissue labeling Flotillin 2/ESA with Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 at 1/1000 (0.535 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on (A) wild-type HEK293T cell pellet, no staining on (B) FLOT2 knockout HEK293T cell pellet. The section was incubated with Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Flow Cytometry (Intracellular) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--flow-cytometry-intracellular-img405520.jpg "Flow Cytometry (Intracellular) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)")
Flow Cytometry (Intracellular) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)
This data was developed using Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized Wild-type 293T (human embryonic kidney epithelial cell, Right) / Flotillin 2/ESA knockout 293T (Left) cells lABelling Flotillin 2/ESA with Anti-Flotillin 2/ESA antibody [EPR26975-52] ab307422 at 1/50 dilution (1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Positive staining on 293T cells (Human wild-type HEK-293T cell line ab255449), while no staining on Flotillin 2/ESA knockout 293T cells (Human FLOT2 (Flotillin 2/ESA) knockout HEK-293T cell line ab266847).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405522.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405521.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)")
![Flow Cytometry (Intracellular) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423), expandable thumbnail](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr26975-52-bsa-and-azide-free-ab307423--flow-cytometry-intracellular-img405520.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Flotillin 2/ESA antibody [EPR26975-52] - BSA and Azide free (ab307423)")
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