Flow cytometry antibodies

Flow cytometry enables quantitative analysis of physical and chemical characteristics of cells or particles as they flow in a stream through a beam of light. The technique is widely used for immunophenotyping, assessing cellular compositions of samples, evaluating cellular function and health, and isolating selected cells by sorting and in biomarker and drug discovery.

To help with your various research needs, we offer a wide range of flow cytometry antibodies:

Directly conjugated primary antibodies

Conjugated antibodies labeled with fluorescent dyes enable direct staining protocols, saving you valuable time by eliminating the need for secondary antibodies. This minimizes potential errors and variability, giving you consistent, reliable results. Additionally, conjugated antibodies enable easy multiplexing in a single step, allowing simultaneous detection of multiple targets within a single sample, which enhances data richness and efficiency.

Our directly conjugated antibodies are tagged with bright, stable fluorophores, ensuring reliable signal detection and reproducibility. These antibodies are particularly beneficial in multicolor experiments where spectral overlap can be a concern, and they help in simplifying the staining process by eliminating the need for secondary antibodies.

Carrier-free antibodies for a conjugation-ready format

Carrier-free antibodies offer flexibility in conjugation, allowing easy labeling to various labels, like fluorophores, enzymes, or proteins, to meet specific experimental needs. These antibodies are also highly compatible with applications where carrier proteins might interfere, such as functional assays, in vivo studies, or when combined with other proteins or peptides. 

Carrier-free antibodies are specifically designed for antibody labeling, saving you time with a simplified protocol. The antibody is added directly to the conjugation reaction, avoiding antibody loss without the need to perform buffer exchange or concentration.

By combining carrier-free antibodies with our Lightning-Link® kits, conjugation has never been easier.

 
Unconjugated primary antibodies

You can use these antibodies with secondary antibodies of your choice, which is useful for applications requiring signal amplification or those involving rare antigens.

What are flow cytometry antibodies used for?

In research and clinical settings, flow cytometry antibodies are essential for:

• Immunology – Characterizing immune cell subsets (e.g., CD4, CD8, CD19).

• Cancer research – Identifying tumor markers and monitoring therapeutic responses.

• Stem cell biology – Tracking differentiation and lineage-specific markers.

• Drug discovery – Evaluating cellular responses to novel compounds.