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Rabbit Recombinant Monoclonal FLRT3 antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-FLRT3 antibody [EPR29132-87] (AB323410), expandable thumbnail
  • Western blot - Anti-FLRT3 antibody [EPR29132-87] (AB323410), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-FLRT3 antibody [EPR29132-87] (AB323410), expandable thumbnail
  • Western blot - Anti-FLRT3 antibody [EPR29132-87] (AB323410), expandable thumbnail
  • Western blot - Anti-FLRT3 antibody [EPR29132-87] (AB323410), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-FrIHC-P
Human
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Tested
Not recommended
Not recommended
Rat
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Functions in cell-cell adhesion, cell migration and axon guidance, exerting an attractive or repulsive role depending on its interaction partners (PubMed:19056886, PubMed:25374360). Plays a role in the spatial organization of brain neurons (PubMed:25374360). Plays a role in vascular development in the retina (PubMed:25374360). Plays a role in cell-cell adhesion via its interaction with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells (PubMed:22405201, PubMed:25374360). Interaction with the intracellular domain of ROBO1 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5B, and possibly also other UNC-5 family members (PubMed:21673655, PubMed:25374360). Promotes neurite outgrowth (in vitro) (By similarity). Mediates cell-cell contacts that promote an increase both in neurite number and in neurite length (By similarity). Plays a role in the regulation of the density of glutamaergic synapses (PubMed:22405201). Plays a role in fibroblast growth factor-mediated signaling cascades (PubMed:16872596). Required for normal morphogenesis during embryonic development, but not for normal embryonic patterning (PubMed:19056886). Required for normal ventral closure, headfold fusion and definitive endoderm migration during embryonic development (PubMed:18448090). Required for the formation of a normal basement membrane and the maintenance of a normal anterior visceral endoderm during embryonic development (PubMed:19056886).

Additional Targets

Flrt3, Flrt3

Alternative names

Kiaa1469, Leucine-rich repeat transmembrane protein FLRT3, Fibronectin leucine rich transmembrane protein 3

Rabbit Recombinant Monoclonal FLRT3 antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29132-87
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for mouse/rat ICC

Unsuitable for WB in human tissues.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410), expandable thumbnail

    Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lanes 1-2: 103 s; Lanes 3-4: 148 s

    This antibody was tested on fresh and frozen MEF and 4T1 whole cell lysate and it was concluded that FLRT3 is the easy target for degradation.

    To minimize protein degradation, cells (lanes 3-4) were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    All lanes: Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410) at 1/1000 dilution

    Lane 1: MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

    Lane 2: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: MEF (mouse embryo fibroblast) fresh whole cell lysate at 20 µg

    Lane 4: 4T1 (mouse mammary gland carcinoma epithelial cell) fresh whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 75-100 kDa, 36 kDa

  • Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410), expandable thumbnail

    Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with mouse FLRT1 and FLRT2.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a mouse FLRT3 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a mouse FLRT1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a mouse FLRT2 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 75-100 kDa, 36 kDa

    Exposure time: 1s

  • Immunocytochemistry/ Immunofluorescence - Anti-FLRT3 antibody [EPR29132-87] (ab323410), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FLRT3 antibody [EPR29132-87] (ab323410)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labelling FLRT3 with ab323410 at 1/100 (5.04 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in Huh7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Negative control: Jurkat.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

  • Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410), expandable thumbnail

    Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: heart, spleen(PMID: 10644439; PMID: 16872596)

    The identity of the bands below 75 kDa are unkown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 40 µg

    Lane 2: Mouse cerebellum tissue lysate at 40 µg

    Lane 3: Mouse hippocampus tissue lysate at 40 µg

    Lane 4: Mouse spleen tissue lysate at 40 µg

    Lane 5: Mouse heart tissue lysate at 40 µg

    Lane 6: Rat brain tissue lysate at 40 µg

    Lane 7: Rat P5 brain tissue lysate at 40 µg

    Lane 8: Rat spleen tissue lysate at 40 µg

    Lane 9: Rat heart tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 75-100 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410), expandable thumbnail

    Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    FLRT3 is a glycoprotein of approximately 80-100 kDa and detected as a 75 kDa band after treated with Deglycosylation Peptide:N-glycosidase F (PNGase F).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410) at 1/1000 dilution

    Lane 1: Untreated Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Huh7 whole cell lysate treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 75-100 kDa, 36 kDa

    Exposure time: 15s

  • Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410), expandable thumbnail

    Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Jurkat.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 21673655, PMID: 24560577).

    The identity of the bands around 50 kDa and 37KDa in lane 3 are unkown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-FLRT3 antibody [EPR29132-87] (ab323410) at 1/1000 dilution

    Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: LN-229 (human brain glioblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Huh7 (human hepatocelullar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 75-100 kDa, 36 kDa

    Exposure time: 114s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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