JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB262844

Anti-Flt 3 / Flk 2 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal Flt3 / CD135 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FLT3 aa 850-1000.

View Alternative Names

CD135, FLK2, STK1, FLT3, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase-2, Fms-like tyrosine kinase 3, Stem cell tyrosine kinase 1, FLK-2, FLT-3, STK-1

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Flt 3 / Flk 2 antibody (AB262844)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Flt 3 / Flk 2 antibody (AB262844)

PFA-fixed, Triton X-100 permeabilized human U-2OS cells stained for Flt 3 / Flk 2 (green) using ab262844 at 4 µg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FLT3 aa 850-1000. The exact immunogen used to generate this antibody is proprietary information.

P36888

style
left-column

Complementary Products

Assay Kits

AB275551

Mouse Flt3 ligand ELISA Kit (Flt3L)

Recombinant|SimpleStep

Sandwich ELISA - Mouse Flt3 ligand ELISA Kit (Flt3L) (AB275551)

  • 0
  • 0
Primary Antibodies

AB52648

Anti-Flt3 ligand/Flt3L antibody [EP1140Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (AB52648)

  • 4
  • 3
Primary Antibodies

AB269456

Anti-CD33 antibody [EPR23051-101]

BOND RX™ Validated|20ul selling size|RabMAb|KO Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [EPR23051-101] (AB269456)

  • 5
  • 5
Primary Antibodies

AB32364

Anti-STAT5 (phospho Y694) antibody [E208]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (AB32364)

  • 4
  • 11
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Flt3 also known as Flk2 is a receptor tyrosine kinase with a molecular mass of about 160 kDa. It is expressed on the surface of hematopoietic stem and progenitor cells mainly within the bone marrow and early lymphoid organs. Flt3 plays an essential mechanical role in the regulation of hematopoietic cell proliferation differentiation and survival. It can bind the Flt3 ligand to initiate downstream signaling which contributes to various cellular responses.
Biological function summary

Flt3 is involved in the early stages of the hematopoiesis process. It does not act as part of a major complex but collaborates with other cytokine receptors to influence cell fate decisions. The receptor's primary role is the regulation of blood cell formation effectively controlling the development and maintenance of certain lymphoid and myeloid lineages. This role establishes Flt3 as a critical factor in ensuring the proper balance of cell populations during immune responses and hematopoietic recovery.

Pathways

Cell proliferation and survival signaling heavily involve Flt3. It activates the PI3K/Akt and the Ras/MAPK signaling pathways upon ligand binding. These pathways are pivotal for normal cellular responses and are shared with related receptor kinases like c-Kit and PDGFR. Flt3 signaling influences multiple aspects of cellular function and interacts with various proteins to modulate hematopoietic processes.

Flt3 mutations or overexpression are strongly associated with acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). Mutations often result in uncontrolled cell proliferation due to constant signaling activity. Flt3's role connects with other proto-oncogenes and tumor suppressor proteins involved in hematopoietic malignancies such as c-Kit and RAS. Understanding these interactions helps in designing targeted therapies for leukemia making Flt3 a significant therapeutic target.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
See full target information FLT3
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com