AB326101

Anti-FLT3 antibody [EPR31042-551]

RabMAb
Recombinant
20ul selling size
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Rabbit Recombinant Monoclonal Flt3 / CD135 antibody. Suitable for WB and reacts with Mouse samples.

View Alternative Names

CD135, Flk-2, Flt-3, Flt3, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase 2, Fms-like tyrosine kinase 3, Tyrosine-protein kinase receptor flk-2, FLK-2, FLT-3

3 Images

Lab

Western blot - Anti-FLT3 antibody [EPR31042-551] (AB326101)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

FLT3 is a glycoprotein of approximately 130-160 kDa and is detected as a 120 kDa band after treatment with the deglycosylation enzyme Peptide : N-glycosidase F (PNGase F).

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 28895560).

Samples are non-boiled as boiling may cause protein aggregation.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-FLT3 antibody [EPR31042-551] (ab326101) at 1/1000 dilution

Lane 1:

Untreated EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 48 µg

Lane 2:

EL4 lysate treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130-160 kDa,120 kDa,124 kDa

true

Exposure time: 180s

Lab

Western blot - Anti-FLT3 antibody [EPR31042-551] (AB326101)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : heart, kidney (PMID : 18031378), B16-F10, Neuro-2a, F9.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 28895560).

Samples are non-boiled as boiling may cause protein aggregation.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-FLT3 antibody [EPR31042-551] (ab326101) at 1/1000 dilution

Lane 1:

Mouse brain membrane fraction at 48 µg

Lane 2:

Mouse thymus membrane fraction at 48 µg

Lane 3:

Mouse heart membrane fraction at 48 µg

Lane 4:

Mouse kidney membrane fraction at 48 µg

Lane 5:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 48 µg

Lane 6:

B16-F0 (mouse melanoma epithelial-like cell) whole cell lysate at 48 µg

Lane 7:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 48 µg

Lane 8:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130-160 kDa,124 kDa

true

Exposure time: 180s

Lab

Western blot - Anti-FLT3 antibody [EPR31042-551] (AB326101)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 28895560).

Samples are non-boiled as boiling may cause protein aggregation.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa); Anti-Integrin alpha V antibody [EPR16800](1 : 5000)(125/135KDa).

All lanes:

Western blot - Anti-FLT3 antibody [EPR31042-551] (ab326101) at 1/1000 dilution

Lane 1:

Mouse brain membrane fraction at 48 µg

Lane 2:

Mouse brain total tissue lysate at 48 µg

Lane 3:

Mouse thymus membrane fraction at 48 µg

Lane 4:

Mouse thymus total tissue lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130-160 kDa,124 kDa,125-135 kDa

true

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR31042-551

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

We recommend the use of high-sensitivity ECL substrate for western blot. Membrane-enriched fractions are recommended for tissue samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

See full target information Flt3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com