AB73019

Anti-Flt3 / CD135 antibody [RM0021-12B6]

Name: Anti-Flt3 / CD135 antibody [RM0021-12B6] (ab73019) | Abcam
Brand: Abcam Limited
SKU: AB73019

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(2 Publications)

Rat Monoclonal Flt3 / CD135 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Flt3.

View Alternative Names

CD135, Flk-2, Flt-3, Flt3, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase 2, Fms-like tyrosine kinase 3, Tyrosine-protein kinase receptor flk-2, FLK-2, FLT-3

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 / CD135 antibody [RM0021-12B6] (AB73019)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 / CD135 antibody [RM0021-12B6] (AB73019)

ab73019 at 1/100 dilution, staining Flt3 / CD135 in LPS treated mouse tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RM0021-12B6

Isotype

IgG2

Carrier free

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Mouse Flt3. The exact immunogen used to generate this antibody is proprietary information.

Q00342

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with PBS at 500µL

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.Filter sterilised.

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Flt3 also known as CD135 or FLT3 tyrosine kinase is a receptor tyrosine kinase that belongs to the class III receptor tyrosine kinase family. It has a molecular weight of approximately 160 kDa. This protein plays an important role in the development of hematopoietic stem and progenitor cells. Flt3 is expressed mainly in early progenitor cells within the bone marrow. Additionally it is present in some populations of mature cells in the peripheral blood and lymphoid organs.

Biological function summary

Flt3 functions as a receptor that is activated by binding to its ligand FLT3 ligand (FLT3L). This interaction stimulates the associated tyrosine kinase activity leading to auto-phosphorylation of Flt3 and subsequent downstream signaling. Flt3 does not form a heterocomplex rather it dimerizes upon ligand binding to initiate signaling cascades. These cascades promote cell proliferation differentiation and survival particularly impacting hematopoiesis and immune responses.

Pathways

Flt3 signaling impacts the internal signaling networks involving the MAPK/ERK and PI3K/AKT pathways both central to cell cycle regulation and apoptosis. Flt3 interacts functionally with proteins such as SHP2 and GRB2 in these pathways aiding signal transduction. The Flt3 receptor also shares functional motifs with KIT and PDGF receptors indicating shared regulatory mechanisms involved in cellular proliferation.

Flt3 mutations especially internal tandem duplications in Flt3/ITD are significant in acute myeloid leukemia (AML) leading to abnormal cell growth and survival. These mutations often co-occur with other genetic anomalies such as NPM1 mutations contributing to the oncogenic process. The abnormal activation of Flt3 kinase activity also has ramifications in myelodysplastic syndromes making it a target for therapeutic intervention with drugs such as anti-FLT3 inhibitors in the treatment regime.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

See full target information Flt3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Brain : a journal of neurology 143:266-288 PubMed31848580

2019

Microglial depletion prevents extracellular matrix changes and striatal volume reduction in a model of Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua D Crapser,Joseph Ochaba,Neelakshi Soni,Jack C Reidling,Leslie M Thompson,Kim N Green

Proceedings of the National Academy of Sciences of the United States of America 112:E6644-53 PubMed26627255

2015

Murine germinal center B cells require functional Fms-like tyrosine kinase 3 signaling for IgG1 class-switch recombination.

Applications

IHC

Species

Mouse

Mattias N D Svensson,Karin M E Andersson,Caroline Wasén,Malin C Erlandsson,Merja Nurkkala-Karlsson,Ing-Marie Jonsson,Mikael Brisslert,Mats Bemark,Maria I Bokarewa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Flt3 / CD135 antibody [RM0021-12B6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 / CD135 antibody [RM0021-12B6] (AB73019)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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