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Rabbit Recombinant Monoclonal Flt3 / CD135 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-Flt3 / CD135 Antibody [YCA-26627-SBC-12] - BSA and Azide free (Detector) (AB323595), expandable thumbnail
  • Sandwich ELISA - Anti-Flt3 / CD135 Antibody [YCA-26627-SBC-12] - BSA and Azide free (Detector) (AB323595), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

Alternative names

CD135, FLK2, STK1, FLT3, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase-2, Fms-like tyrosine kinase 3, Stem cell tyrosine kinase 1, FLK-2, FLT-3, STK-1

Rabbit Recombinant Monoclonal Flt3 / CD135 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
YCA-26627-SBC-12
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab323595 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Flt3 / CD135. The recommended pair for sandwich ELISA is:
Capture: Anti-Flt3 / CD135 Antibody [YCA-26627-SBC-11] - BSA and Azide free (Capture) ab323596, Human Flt3 / CD135 Capture Antibody (unconjugated)
Detector: ab323595, Human Flt3 / CD135 Detector Antibody (unconjugated)
The reference range value is 9.7-312 pg/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Anti-Flt3 / CD135 Antibody [YCA-26627-SBC-12] - BSA and Azide free (Detector) (ab323595), expandable thumbnail

    Sandwich ELISA - Anti-Flt3 / CD135 Antibody [YCA-26627-SBC-12] - BSA and Azide free (Detector) (ab323595)

    Sandwich ELISA of ab323592 with the capture antibody Anti-Flt3 / CD135 Antibody [YCA-26627-SBC-11] - BSA and Azide free (Capture) ab323596 at 2 μg/mL and detector antibody ab323595 at 0.5 μg/mL. Background-subtracted absorbance data values (mean +/- standard deviation) are displayed.

  • Sandwich ELISA - Anti-Flt3 / CD135 Antibody [YCA-26627-SBC-12] - BSA and Azide free (Detector) (ab323595), expandable thumbnail

    Sandwich ELISA - Anti-Flt3 / CD135 Antibody [YCA-26627-SBC-12] - BSA and Azide free (Detector) (ab323595)

    Interpolated concentration of FLT3 / CD135 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: THP-1 cell extract 15.625 µg/ml and HeLa cell extract 15.625 µg/ml. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% Bovine Serum Albumin (BSA). In the HeLa cell extract the signal was non-detectable (ND). Data represented here are indicative of assay working using example of biological samples. However, we do not guarantee same sensitivity level due to biological variation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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