JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB231192

Anti-Flt3 ligand/Flt3L antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal Flt3 ligand/Flt3L antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Flt3lg aa 1-200.

View Alternative Names

Flt3l, Flt3lg, Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine

2 Images
Western blot - Anti-Flt3 ligand/Flt3L antibody (AB231192)
  • WB

Supplier Data

Western blot - Anti-Flt3 ligand/Flt3L antibody (AB231192)

All lanes:

Western blot - Anti-Flt3 ligand/Flt3L antibody (ab231192) at 2 µg/mL

All lanes:

Recombinant mouse Flt3 ligand / Flt3L protein

Predicted band size: 17 kDa,26 kDa,38 kDa,63 kDa

false

Western blot - Anti-Flt3 ligand/Flt3L antibody (AB231192)
  • WB

Supplier Data

Western blot - Anti-Flt3 ligand/Flt3L antibody (AB231192)

All lanes:

Western blot - Anti-Flt3 ligand/Flt3L antibody (ab231192) at 1 µg/mL

Lane 1:

Mouse lung tissue lysate

Lane 2:

Mouse testis tissue lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Flt3lg aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P49772

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Flt3 ligand (Flt3L) also known as Flt3-L Flt3 ligand protein or flt-3 ligand protein is a small cytokine with a molecular mass of approximately 18 to 19 kDa. It acts as a growth factor by binding to the Flt3 receptor tyrosine kinase on the surface of hematopoietic progenitor cells. Flt3L is expressed in various tissues including the bone marrow where it plays a significant role in hematopoiesis. It is critical in dendritic cell and B cell development impacting the immune response mechanism.
Biological function summary

The Flt3 ligand plays an essential role in the immune system. It is a monomeric cytokine that induces proliferation of early lineage cells within the hematopoietic system. Flt3L does not function as part of a complex but acts independently to stimulate the proliferation differentiation and survival of hematopoietic cells. By interacting directly with its receptor it significantly influences the innate and adaptive immune systems through the maturation of dendritic and B cells.

Pathways

The Flt3 ligand is involved in hematopoietic signaling pathways primarily the PI3K-Akt signaling pathway. This pathway is essential for cell survival and growth where Flt3L aids in transmitting growth signals necessary for hematopoietic cells. Flt3L also interacts with proteins such as FLT3 receptor and influences the downstream signaling cascade affecting cell fate decisions in hematopoiesis and immune cell maturation.

Flt3 ligand is connected to leukemia particularly acute myeloid leukemia (AML) due to dysregulation of the FLT3 signaling pathway. Mutations in the FLT3 receptor can lead to uncontrolled cell proliferation contributing to tumorigenesis. Additionally Flt3L plays a role in autoimmune disorders by influencing immune cell development. It is closely connected with the FLT3 receptor and other tyrosine kinases which when mutated can exacerbate disease pathology through abnormal cell signaling and proliferation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
See full target information Flt3lg
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7466 PubMed36463242

2022

Engineered Lactococcus lactis secreting Flt3L and OX40 ligand for in situ vaccination-based cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Junmeng Zhu,Yaohua Ke,Qin Liu,Ju Yang,Fangcen Liu,Ruihan Xu,Hang Zhou,Aoxing Chen,Jie Xiao,Fanyan Meng,Lixia Yu,Rutian Li,Jia Wei,Baorui Liu
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB84261

Biotin Anti-Flt3 ligand/Flt3L antibody

Sandwich ELISA - Biotin Anti-Flt3 ligand/Flt3L antibody (AB84261)

  • 0
  • 0
Primary Antibodies

AB231249

Anti-Flt3 ligand/Flt3L antibody

Western blot - Anti-Flt3 ligand/Flt3L antibody (AB231249)

  • 0
  • 0
Primary Antibodies

AB238610

Anti-Flt3 ligand/Flt3L antibody

Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody (AB238610)

  • 0
  • 0
Primary Antibodies

AB52648

Anti-Flt3 ligand/Flt3L antibody [EP1140Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (AB52648)

  • 4
  • 3
Primary Antibodies

AB232487

Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (AB232487)

  • 0
  • 0

Complementary Products

Assay Kits

AB254507

Human Flt-3 ligand ELISA Kit

Recombinant|SimpleStep

ELISA - Human Flt-3 ligand ELISA Kit (AB254507)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB270071

Recombinant mouse Flt3 ligand/Flt3L protein (Active)

Premium Bioactive

Functional Studies - Recombinant mouse Flt3 ligand/Flt3L protein (Active) (AB270071)

  • 0
  • 0
Primary Antibodies

AB208687

Anti-FGF2 antibody [EPR20145-219]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-FGF2 antibody [EPR20145-219] (AB208687)

  • 5
  • 3
Primary Antibodies

AB209216

Anti-TLR2 antibody [EPR20302-119]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] (AB209216)

  • 0
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com