AB9688

Anti-Flt3 ligand/Flt3L antibody

Name: Anti-Flt3 ligand/Flt3L antibody (ab9688) | Abcam
Brand: Abcam Limited
SKU: AB9688
Price: 345 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Flt3 ligand/Flt3L antibody. Suitable for Flow Cyt, WB, sELISA and reacts with Recombinant fragment, Human, Recombinant full length protein - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FLT3LG aa 1-200.

View Alternative Names

Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine, FLT3LG

1 Images

Sandwich ELISA - Anti-Flt3 ligand/Flt3L antibody (AB9688)

sELISA

Supplier Data

Sandwich ELISA - Anti-Flt3 ligand/Flt3L antibody (AB9688)

To detect human Flt3 ligand/Flt3L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab84261 is required. This biotinylated polyclonal antibody, in conjunction with an anti-Human Flt3-ligand (ab9688) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human Flt3 ligand/Flt3L.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

sELISA, Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human FLT3LG aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P49771

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "The detection limit for recombinant Flt3 ligand/Flt3L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant fragment": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "The detection limit for recombinant Flt3 ligand/Flt3L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.5-2 µg/mL", "sELISA-species-notes": "In conjunction with <a href='/en-us/products/primary-antibodies/biotin-flt3-ligand-flt3l-antibody-ab84261'>ab84261</a> rabbit polyclonal to Flt3 ligand/Flt3L (Biotin) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Flt3 ligand/Flt3L." } } }

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Product details

hflt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Immunogen

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Flt3 ligand (Flt3L) also known as Flt3-L Flt3 ligand protein or flt-3 ligand protein is a small cytokine with a molecular mass of approximately 18 to 19 kDa. It acts as a growth factor by binding to the Flt3 receptor tyrosine kinase on the surface of hematopoietic progenitor cells. Flt3L is expressed in various tissues including the bone marrow where it plays a significant role in hematopoiesis. It is critical in dendritic cell and B cell development impacting the immune response mechanism.

Biological function summary

The Flt3 ligand plays an essential role in the immune system. It is a monomeric cytokine that induces proliferation of early lineage cells within the hematopoietic system. Flt3L does not function as part of a complex but acts independently to stimulate the proliferation differentiation and survival of hematopoietic cells. By interacting directly with its receptor it significantly influences the innate and adaptive immune systems through the maturation of dendritic and B cells.

Pathways

The Flt3 ligand is involved in hematopoietic signaling pathways primarily the PI3K-Akt signaling pathway. This pathway is essential for cell survival and growth where Flt3L aids in transmitting growth signals necessary for hematopoietic cells. Flt3L also interacts with proteins such as FLT3 receptor and influences the downstream signaling cascade affecting cell fate decisions in hematopoiesis and immune cell maturation.

Flt3 ligand is connected to leukemia particularly acute myeloid leukemia (AML) due to dysregulation of the FLT3 signaling pathway. Mutations in the FLT3 receptor can lead to uncontrolled cell proliferation contributing to tumorigenesis. Additionally Flt3L plays a role in autoimmune disorders by influencing immune cell development. It is closely connected with the FLT3 receptor and other tyrosine kinases which when mutated can exacerbate disease pathology through abnormal cell signaling and proliferation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. Required for the development of B cells, and dendritic cells (DCs).

See full target information FLT3LG

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:2498 PubMed31175312

2019

Mesenchymal stem cell therapy induces FLT3L and CD1c dendritic cells in systemic lupus erythematosus patients.

Applications

Unspecified application

Species

Unspecified reactive species

Xinran Yuan,Xiaodong Qin,Dandan Wang,Zhuoya Zhang,Xiaojun Tang,Xiang Gao,Wanjun Chen,Lingyun Sun

Nature medicine 24:1178-1191 PubMed29942093

2018

A natural killer-dendritic cell axis defines checkpoint therapy-responsive tumor microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin C Barry,Joy Hsu,Miranda L Broz,Francisco J Cueto,Mikhail Binnewies,Alexis J Combes,Amanda E Nelson,Kimberly Loo,Raj Kumar,Michael D Rosenblum,Michael D Alvarado,Denise M Wolf,Dusan Bogunovic,Nina Bhardwaj,Adil I Daud,Patrick K Ha,William R Ryan,Joshua L Pollack,Bushra Samad,Saurabh Asthana,Vincent Chan,Matthew F Krummel

BMC immunology 13:46 PubMed22897934

2012

Regulation of in vitro human T cell development through interleukin-7 deprivation and anti-CD3 stimulation.

Applications

Flow Cyt

Species

Human

Ekta S Patel,Starlyn Okada,Kevin Hachey,Li-jun Yang,Scott K Durum,Jan S Moreb,Lung-Ji Chang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Flt3 ligand/Flt3L antibody

Sandwich ELISA - Anti-Flt3 ligand/Flt3L antibody (AB9688)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Reconstitution

Purification technique

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise