Rabbit Polyclonal Flt3 ligand/Flt3L antibody. Suitable for Flow Cyt, WB, sELISA, Neut and reacts with Recombinant fragment, Human, Recombinant full length protein - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FLT3LG aa 1-200.
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Publications
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- Nature communications 10:24982019Mesenchymal stem cell therapy induces FLT3L and CD1c dendritic cells in systemic lupus erythematosus patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinran Yuan et. al.PubMed 31175312
- Nature medicine 24:1178-11912018A natural killer-dendritic cell axis defines checkpoint therapy-responsive tumor microenvironments.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKevin C Barry,Joy Hsu,Miranda L Broz,Francisco J Cueto,Mikhail Binnewies,Alexis J Combes,Amanda E Nelson,Kimberly Loo,Raj Kumar,Michael D Rosenblum,Michael D Alvarado,Denise M Wolf,Dusan Bogunovic,Nina Bhardwaj,Adil I Daud,Patrick K Ha,William R Ryan,Joshua L Pollack,Bushra Samad,Saurabh Asthana,Vincent Chan,Matthew F KrummelPubMed 29942093
- BMC immunology 13:462012Regulation of in vitro human T cell development through interleukin-7 deprivation and anti-CD3 stimulation.Applications:Flow CytReactive species:HumanEkta S Patel,Starlyn Okada,Kevin Hachey,Li-jun Yang,Scott K Durum,Jan S Moreb,Lung-Ji ChangPubMed 22897934
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human FLT3LG aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P49771
Reactivity data
Select an application| Flow Cyt | WB | sELISA | Neut | |
|---|---|---|---|---|
| Human | Expected | Expected | Predicted | Expected |
| Recombinant fragment | Expected | Expected | Not recommended | Expected |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested | Not recommended |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes The detection limit for recombinant Flt3 ligand/Flt3L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Species Human | Dilution info - | Notes The detection limit for recombinant Flt3 ligand/Flt3L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.5-2 µg/mL | Notes In conjunction with Biotin Anti-Flt3 ligand/Flt3L antibody ab84261 rabbit polyclonal to Flt3 ligand/Flt3L (Biotin) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Flt3 ligand/Flt3L. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Neut
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes Yields one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/ml) |
Species Human | Dilution info - | Notes Yields one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/ml) |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Alternative names
Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine, FLT3LG
Recommended products
Rabbit Polyclonal Flt3 ligand/Flt3L antibody. Suitable for Flow Cyt, WB, sELISA, Neut and reacts with Recombinant fragment, Human, Recombinant full length protein - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FLT3LG aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human FLT3LG aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P49771
- Purification technique
- Affinity purification Immunogen
- Reconstitution
- reconstitute with water at 200µL
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
hflt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Flt3 ligand (Flt3L) also known as Flt3-L Flt3 ligand protein or flt-3 ligand protein is a small cytokine with a molecular mass of approximately 18 to 19 kDa. It acts as a growth factor by binding to the Flt3 receptor tyrosine kinase on the surface of hematopoietic progenitor cells. Flt3L is expressed in various tissues including the bone marrow where it plays a significant role in hematopoiesis. It is critical in dendritic cell and B cell development impacting the immune response mechanism.
- Biological function summary
The Flt3 ligand plays an essential role in the immune system. It is a monomeric cytokine that induces proliferation of early lineage cells within the hematopoietic system. Flt3L does not function as part of a complex but acts independently to stimulate the proliferation differentiation and survival of hematopoietic cells. By interacting directly with its receptor it significantly influences the innate and adaptive immune systems through the maturation of dendritic and B cells.
- Pathways
The Flt3 ligand is involved in hematopoietic signaling pathways primarily the PI3K-Akt signaling pathway. This pathway is essential for cell survival and growth where Flt3L aids in transmitting growth signals necessary for hematopoietic cells. Flt3L also interacts with proteins such as FLT3 receptor and influences the downstream signaling cascade affecting cell fate decisions in hematopoiesis and immune cell maturation.
- Associated diseases and disorders
Flt3 ligand is connected to leukemia particularly acute myeloid leukemia (AML) due to dysregulation of the FLT3 signaling pathway. Mutations in the FLT3 receptor can lead to uncontrolled cell proliferation contributing to tumorigenesis. Additionally Flt3L plays a role in autoimmune disorders by influencing immune cell development. It is closely connected with the FLT3 receptor and other tyrosine kinases which when mutated can exacerbate disease pathology through abnormal cell signaling and proliferation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Sandwich ELISA - Anti-Flt3 ligand/Flt3L antibody (ab9688)
To detect human Flt3 ligand/Flt3L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of Biotin Anti-Flt3 ligand/Flt3L antibody ab84261 is required. This biotinylated polyclonal antibody, in conjunction with an anti-Human Flt3-ligand (ab9688) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human Flt3 ligand/Flt3L.
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