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Rabbit Recombinant Monoclonal Flt3 ligand/Flt3L antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (AB52648), expandable thumbnail
  • Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (AB52648), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (AB52648), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (AB52648), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Rhesus monkey
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/180
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Rhesus monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100000
Notes

-

Predicted
Predicted

Species
Rhesus monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Predicted
Predicted

Species
Rhesus monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Rhesus monkey
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

Alternative names

Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine, FLT3LG

Recommended products

Rabbit Recombinant Monoclonal Flt3 ligand/Flt3L antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1140Y
Purification technique
Affinity purification Protein A
Specificity

The antibody has been tested against recombinant protein in western blotting, we do not have data regarding recognition of endogenous protein. We suggest K562 and HL-60 cells could be expressing Flt3 ligand/Flt3L. The antibody was raised against the extracellular domain of the protein, however please note permeabilisation in FACS greatly improves the signal.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Flt3 ligand (Flt3L) also known as Flt3-L Flt3 ligand protein or flt-3 ligand protein is a small cytokine with a molecular mass of approximately 18 to 19 kDa. It acts as a growth factor by binding to the Flt3 receptor tyrosine kinase on the surface of hematopoietic progenitor cells. Flt3L is expressed in various tissues including the bone marrow where it plays a significant role in hematopoiesis. It is critical in dendritic cell and B cell development impacting the immune response mechanism.

Biological function summary

The Flt3 ligand plays an essential role in the immune system. It is a monomeric cytokine that induces proliferation of early lineage cells within the hematopoietic system. Flt3L does not function as part of a complex but acts independently to stimulate the proliferation differentiation and survival of hematopoietic cells. By interacting directly with its receptor it significantly influences the innate and adaptive immune systems through the maturation of dendritic and B cells.

Pathways

The Flt3 ligand is involved in hematopoietic signaling pathways primarily the PI3K-Akt signaling pathway. This pathway is essential for cell survival and growth where Flt3L aids in transmitting growth signals necessary for hematopoietic cells. Flt3L also interacts with proteins such as FLT3 receptor and influences the downstream signaling cascade affecting cell fate decisions in hematopoiesis and immune cell maturation.

Associated diseases and disorders

Flt3 ligand is connected to leukemia particularly acute myeloid leukemia (AML) due to dysregulation of the FLT3 signaling pathway. Mutations in the FLT3 receptor can lead to uncontrolled cell proliferation contributing to tumorigenesis. Additionally Flt3L plays a role in autoimmune disorders by influencing immune cell development. It is closely connected with the FLT3 receptor and other tyrosine kinases which when mutated can exacerbate disease pathology through abnormal cell signaling and proliferation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Flt3 ligand/Flt3L with Purified ab52648 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648), expandable thumbnail

    Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648) at 1/100000 dilution

    All lanes: Human Flt3 ligand/Flt3L (aa 1 to 235) recombinant protein, 15ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Flt3 ligand with purified ab52648 at 1:180 dilution (2.9 μg/ml). Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Flt3 ligand/Flt3L with ab52648 at 1/20 dilution (1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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