Rabbit Recombinant Monoclonal Flt3 ligand/Flt3L antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant full length protein - Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (AB232487), expandable thumbnail](https://content.abcam.com/products/images/flt3-ligand-flt3l-antibody-ep1140y-bsa-and-azide-free-ab232487--immunocytochemistry-immunofluorescence-img66217.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (AB232487)")
![Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (AB232487), expandable thumbnail](https://content.abcam.com/products/images/flt3-ligand-flt3l-antibody-ep1140y-bsa-and-azide-free-ab232487--western-blot-img138620.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (AB232487)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (AB232487), expandable thumbnail](https://content.abcam.com/products/images/flt3-ligand-flt3l-antibody-ep1140y-bsa-and-azide-free-ab232487--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124568.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (AB232487)")
![Flow Cytometry (Intracellular) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (AB232487), expandable thumbnail](https://content.abcam.com/products/images/flt3-ligand-flt3l-antibody-ep1140y-bsa-and-azide-free-ab232487--flow-cytometry-intracellular-img414772.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (AB232487)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376-Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Alternative names
Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine, FLT3LG
Recommended products
Rabbit Recombinant Monoclonal Flt3 ligand/Flt3L antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant full length protein - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1140Y
- Purification technique
- Affinity purification Protein A
- Specificity
The antibody has been tested against recombinant protein in western blotting, we do not have data regarding recognition of endogenous protein. We suggest K562 and HL-60 cells could be expressing Flt3 ligand/Flt3L. The antibody was raised against the extracellular domain of the protein, however please note permeabilisation in FACS greatly improves the signal.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232487 is the carrier-free version of Anti-Flt3 ligand/Flt3L antibody [EP1140Y] ab52648.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Flt3 ligand (Flt3L) also known as Flt3-L Flt3 ligand protein or flt-3 ligand protein is a small cytokine with a molecular mass of approximately 18 to 19 kDa. It acts as a growth factor by binding to the Flt3 receptor tyrosine kinase on the surface of hematopoietic progenitor cells. Flt3L is expressed in various tissues including the bone marrow where it plays a significant role in hematopoiesis. It is critical in dendritic cell and B cell development impacting the immune response mechanism.
- Biological function summary
The Flt3 ligand plays an essential role in the immune system. It is a monomeric cytokine that induces proliferation of early lineage cells within the hematopoietic system. Flt3L does not function as part of a complex but acts independently to stimulate the proliferation differentiation and survival of hematopoietic cells. By interacting directly with its receptor it significantly influences the innate and adaptive immune systems through the maturation of dendritic and B cells.
- Pathways
The Flt3 ligand is involved in hematopoietic signaling pathways primarily the PI3K-Akt signaling pathway. This pathway is essential for cell survival and growth where Flt3L aids in transmitting growth signals necessary for hematopoietic cells. Flt3L also interacts with proteins such as FLT3 receptor and influences the downstream signaling cascade affecting cell fate decisions in hematopoiesis and immune cell maturation.
- Associated diseases and disorders
Flt3 ligand is connected to leukemia particularly acute myeloid leukemia (AML) due to dysregulation of the FLT3 signaling pathway. Mutations in the FLT3 receptor can lead to uncontrolled cell proliferation contributing to tumorigenesis. Additionally Flt3L plays a role in autoimmune disorders by influencing immune cell development. It is closely connected with the FLT3 receptor and other tyrosine kinases which when mutated can exacerbate disease pathology through abnormal cell signaling and proliferation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (ab232487), expandable thumbnail](https://content.abcam.com/products/images/flt3-ligand-flt3l-antibody-ep1140y-bsa-and-azide-free-ab232487--immunocytochemistry-immunofluorescence-img66217.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (ab232487)")
Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (ab232487)
This data was developed using Anti-Flt3 ligand/Flt3L antibody [EP1140Y] ab52648, the same antibody clone in a different buffer formulation.
Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Flt3 ligand/Flt3L with Purified Anti-Flt3 ligand/Flt3L antibody [EP1140Y] ab52648 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (ab232487), expandable thumbnail](https://content.abcam.com/products/images/flt3-ligand-flt3l-antibody-ep1140y-bsa-and-azide-free-ab232487--western-blot-img138620.jpg "Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (ab232487)")
Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (ab232487)
This data was developed using Anti-Flt3 ligand/Flt3L antibody [EP1140Y] ab52648, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (ab232487) at 1/100000 dilution
All lanes: Human Flt3 ligand/Flt3L (aa 1 to 235) recombinant protein, 15 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 26 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (ab232487), expandable thumbnail](https://content.abcam.com/products/images/flt3-ligand-flt3l-antibody-ep1140y-bsa-and-azide-free-ab232487--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124568.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (ab232487)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (ab232487)
This data was developed using Anti-Flt3 ligand/Flt3L antibody [EP1140Y] ab52648, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Flt3 ligand/Flt3L with Purified Anti-Flt3 ligand/Flt3L antibody [EP1140Y] ab52648 at 1:180 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
![Flow Cytometry (Intracellular) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (ab232487), expandable thumbnail](https://content.abcam.com/products/images/flt3-ligand-flt3l-antibody-ep1140y-bsa-and-azide-free-ab232487--flow-cytometry-intracellular-img414772.jpg "Flow Cytometry (Intracellular) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (ab232487)")
Flow Cytometry (Intracellular) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] - BSA and Azide free (ab232487)
This data was developed using Anti-Flt3 ligand/Flt3L antibody [EP1140Y] ab52648, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Flt3 ligand/Flt3L with purified Anti-Flt3 ligand/Flt3L antibody [EP1140Y] ab52648 at 1/50 dilution. Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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