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AB224247

Anti-FMNL1 antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal FMNL1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FMNL1 aa 850 to C-terminus.

View Alternative Names

C17orf1, C17orf1B, FMNL, FRL1, FMNL1, Formin-like protein 1, CLL-associated antigen KW-13, Leukocyte formin

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMNL1 antibody (AB224247)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMNL1 antibody (AB224247)

Paraffin embedded human small intestine tissue stained for FMNL1 with ab224247 (1/50 dilution) in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMNL1 antibody (AB224247)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMNL1 antibody (AB224247)

Paraffin embedded human lymph node tissue stained for FMNL1 with ab224247 (1/50 dilution) in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMNL1 antibody (AB224247)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMNL1 antibody (AB224247)

Paraffin embedded human lung tissue stained for FMNL1 with ab224247 (1/50 dilution) in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-FMNL1 antibody (AB224247)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FMNL1 antibody (AB224247)

PFA-fixed/Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for FMNL1 (green) using ab224247 (4 μg/ml) in ICC/IF.

Western blot - Anti-FMNL1 antibody (AB224247)
  • WB

Supplier Data

Western blot - Anti-FMNL1 antibody (AB224247)

All lanes:

Western blot - Anti-FMNL1 antibody (ab224247) at 1/100 dilution

All lanes:

MOLT-4 (human lymphoblastic leukemia cell line) cell lysate

Predicted band size: 122 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FMNL1 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95466

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

May play a role in the control of cell motility and survival of macrophages (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics and cell shape.
See full target information FMNL1

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:5772 PubMed34599178

2021

Ring finger protein 213 assembles into a sensor for ISGylated proteins with antimicrobial activity.

Applications

Unspecified application

Species

Unspecified reactive species

Fabien Thery,Lia Martina,Caroline Asselman,Yifeng Zhang,Madeleine Vessely,Heidi Repo,Koen Sedeyn,George D Moschonas,Clara Bredow,Qi Wen Teo,Jingshu Zhang,Kevin Leandro,Denzel Eggermont,Delphine De Sutter,Katie Boucher,Tino Hochepied,Nele Festjens,Nico Callewaert,Xavier Saelens,Bart Dermaut,Klaus-Peter Knobeloch,Antje Beling,Sumana Sanyal,Lilliana Radoshevich,Sven Eyckerman,Francis Impens

Journal of Cancer 12:6563-6575 PubMed34659547

2021

Quantitative proteomic analysis reveals sophisticated metabolic alteration and identifies FMNL1 as a prognostic marker in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Gui Ma,Zirui Wang,Junyao Liu,Shengjun Fu,Lili Zhang,Duo Zheng,Panfeng Shang,Zhongjin Yue
View all publications

Product promise

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