Rabbit Recombinant Monoclonal FMO5 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (AB250992), expandable thumbnail](https://content.abcam.com/products/images/fmo5-antibody-epr14783-bsa-and-azide-free-ab250992--western-blot-img81834.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (AB250992)")
![Western blot - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (AB250992), expandable thumbnail](https://content.abcam.com/products/images/fmo5-antibody-epr14783-bsa-and-azide-free-ab250992--western-blot-img94068.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (AB250992)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (AB250992), expandable thumbnail](https://content.abcam.com/products/images/fmo5-antibody-epr14783-bsa-and-azide-free-ab250992--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23132.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (AB250992)")
![Immunoprecipitation - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (AB250992), expandable thumbnail](https://content.abcam.com/products/images/fmo5-antibody-epr14783-bsa-and-azide-free-ab250992--immunoprecipitation-img150426.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (AB250992)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
| Mouse | Predicted | Predicted | Not recommended | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Acts as a Baeyer-Villiger monooxygenase on a broad range of substrates. Catalyzes the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters (PubMed:20947616, PubMed:26771671, PubMed:28783300). Active on diverse carbonyl compounds, whereas soft nucleophiles are mostly non- or poorly reactive (PubMed:26771671, PubMed:7872795). In contrast with other forms of FMO it is non- or poorly active on 'classical' substrates such as drugs, pesticides, and dietary components containing soft nucleophilic heteroatoms (Probable) (PubMed:7872795). Able to oxidize drug molecules bearing a carbonyl group on an aliphatic chain, such as nabumetone and pentoxifylline (PubMed:28783300). Also, in the absence of substrates, shows slow but yet significant NADPH oxidase activity (PubMed:26771671). Acts as a positive modulator of cholesterol biosynthesis as well as glucose homeostasis, promoting metabolic aging via pleiotropic effects (By similarity).
Alternative names
Flavin-containing monooxygenase 5, FMO 5, Baeyer-Villiger monooxygenase 1, Dimethylaniline monooxygenase [N-oxide-forming] 5, Dimethylaniline oxidase 5, NADPH oxidase, hBVMO1, FMO5
Recommended products
Rabbit Recombinant Monoclonal FMO5 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR14783
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250992 is the carrier-free version of Anti-FMO5 antibody [EPR14783] ab189516.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (ab250992), expandable thumbnail](https://content.abcam.com/products/images/fmo5-antibody-epr14783-bsa-and-azide-free-ab250992--western-blot-img81834.jpg "Western blot - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (ab250992)")
Western blot - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (ab250992)
This data was developed using Anti-FMO5 antibody [EPR14783] ab189516, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-FMO5 antibody [EPR14783] (Anti-FMO5 antibody [EPR14783] ab189516) at 1/10000 dilution
All lanes: Human fetal liver tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
![Western blot - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (ab250992), expandable thumbnail](https://content.abcam.com/products/images/fmo5-antibody-epr14783-bsa-and-azide-free-ab250992--western-blot-img94068.jpg "Western blot - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (ab250992)")
Western blot - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (ab250992)
This data was developed using Anti-FMO5 antibody [EPR14783] ab189516, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-FMO5 antibody [EPR14783] (Anti-FMO5 antibody [EPR14783] ab189516) at 1/10000 dilution
All lanes: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (ab250992), expandable thumbnail](https://content.abcam.com/products/images/fmo5-antibody-epr14783-bsa-and-azide-free-ab250992--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23132.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (ab250992)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (ab250992)
This data was developed using Anti-FMO5 antibody [EPR14783] ab189516, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffine embedded Human liver tissue labeling FMO5 with Anti-FMO5 antibody [EPR14783] ab189516 at 1/500 dilution. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (ab250992), expandable thumbnail](https://content.abcam.com/products/images/fmo5-antibody-epr14783-bsa-and-azide-free-ab250992--immunoprecipitation-img150426.jpg "Immunoprecipitation - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (ab250992)")
Immunoprecipitation - Anti-FMO5 antibody [EPR14783] - BSA and Azide free (ab250992)
This data was developed using Anti-FMO5 antibody [EPR14783] ab189516, the same antibody clone in a different buffer formulation.
Immunoprecipitation of Human fetal liver labeling FMO5 with Anti-FMO5 antibody [EPR14783] ab189516 at 1/50 diltion and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 (+) or PBS (-).
All lanes: Immunoprecipitation - Anti-FMO5 antibody [EPR14783] (Anti-FMO5 antibody [EPR14783] ab189516)
Predicted band size: 60 kDa
Observed band size: 60 kDa
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