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AB232766

Anti-FN3K antibody

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(1 Publication)

Rabbit Polyclonal FN3K antibody. Suitable for WB, ICC/IF and reacts with Human, Recombinant full length protein - Human, Pig samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FN3K.

View Alternative Names

Fructosamine-3-kinase, Protein-psicosamine 3-kinase FN3K, Protein-ribulosamine 3-kinase FN3K, FN3K

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-FN3K antibody (AB232766)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FN3K antibody (AB232766)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FN3K (green) using ab232766 at 20 μg/ml in ICC/IF. FITC-labeled secondary antibody.

Western blot - Anti-FN3K antibody (AB232766)
  • WB

Supplier Data

Western blot - Anti-FN3K antibody (AB232766)

All lanes:

Western blot - Anti-FN3K antibody (ab232766) at 2 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 3:

Pig heart lysate

Lane 4:

Pig brain lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 35 kDa

false

Western blot - Anti-FN3K antibody (AB232766)
  • WB

Ap

Western blot - Anti-FN3K antibody (AB232766)

All lanes:

Western blot - Anti-FN3K antibody (ab232766) at 2 µg/mL

All lanes:

Recombinant human FN3K protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FN3K.

Q9H479

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-20 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Fructosamine-3-kinase involved in protein deglycation by mediating phosphorylation of fructoselysine residues on glycated proteins, to generate fructoselysine-3 phosphate (PubMed : 11016445, PubMed : 11522682, PubMed : 11975663). Fructoselysine-3 phosphate adducts are unstable and decompose under physiological conditions (PubMed : 11522682, PubMed : 11975663). Involved in intracellular deglycation in erythrocytes (PubMed : 11975663). Involved in the response to oxidative stress by mediating deglycation of NFE2L2/NRF2, glycation impairing NFE2L2/NRF2 function (By similarity). Also able to phosphorylate psicosamines and ribulosamines (PubMed : 14633848).
See full target information FN3K
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 24:1128-1140 PubMed31755222

2019

Fibroblast growth factor-2/platelet-derived growth factor enhances atherosclerotic plaque stability.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Mao,Xiao Qiong Liu,Yu Song,Chun Gang Zhai,Xing Li Xu,Lei Zhang,Yun Zhang
View all publications
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Product promise

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