AB131390

Anti-FNDC5 antibody

Name: Anti-FNDC5 antibody (ab131390) | Abcam
Brand: Abcam Limited
SKU: AB131390
Price: 350 USD
Availability: InStock
Rating: 2 (1 reviews)

(1 Review)

(21 Publications)

Rabbit Polyclonal FNDC5 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 21 publications.

View Alternative Names

FRCP2, FNDC5, Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2

2 Images

Ap27264****

Western blot - Anti-FNDC5 antibody (AB131390)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab131390 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-FNDC5 antibody (ab131390) at 1 µg/mL

Lane 1:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Lane 2:

C2C12 (Mouse myoblast cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

true

Exposure time: 30s

Lab

Western blot - Anti-FNDC5 antibody (AB131390)

All lanes:

Western blot - Anti-FNDC5 antibody (ab131390) at 1 µg/mL

Lane 1:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Lane 2:

C2C12 (Mouse myoblast cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa,45 kDa,50 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Irisin. Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.

See full target information FNDC5

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:11532-11545 PubMed37878003

2023

Indoxyl sulfate induced frailty in patients with end-stage renal disease by disrupting the PGC-1α-FNDC5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chou Hou,Min-Tser Liao,Kuo-Wang Tsai,Cai-Mei Zheng,Hui-Wen Chiu,Kuo-Cheng Lu

iScience 26:107047 PubMed37360691

2023

Toward countering muscle and bone loss with spaceflight: GSK3 as a potential target.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan W Baranowski,Jessica L Braun,Briana L Hockey,Jenalyn L Yumol,Mia S Geromella,Colton J F Watson,Nigel Kurgan,Holt N Messner,Kennedy C Whitley,Adam J MacNeil,Guillemette Gauquelin-Koch,Fabrice Bertile,William Gittings,Rene Vandenboom,Wendy E Ward,Val A Fajardo

Iranian journal of basic medical sciences 26:717-724 PubMed37275755

2023

Irisin attenuates angiotensin II-induced atrial fibrillation and atrial fibrosis via LOXL2 and TGFβ1/Smad2/3 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yingbiao Wu,Jun Luo,Xiang Song,Wei Gu,Saihua Wang,Shuwen Hao,Zhiwu Dong,Zhongping Ning

Nutrients 15: PubMed37299522

2023

Extract Supplementation Contributes to Improve Aberrant Multi-Organ Energy Metabolism via Muscle-Brain Connectivity in Diabetic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Heaji Lee,Sun Yeou Kim,Yunsook Lim

Journal of translational medicine 21:139 PubMed36823656

2023

Low-intensity pulsed ultrasound triggers a beneficial neuromodulation in dementia mice with chronic cerebral hypoperfusion via activation of hippocampal Fndc5/irisin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Degang Song,Xiaomin Chen,Na Zhou,Yi Yuan,Shuo Geng,Cong Zhang,Zhe Zhao,Xingran Wang,Xinran Bao,Xifa Lan,Xiangjian Zhang

Diabetes 71:2777-2792 PubMed35802043

2022

Exercise-Linked Skeletal Irisin Ameliorates Diabetes-Associated Osteoporosis by Inhibiting the Oxidative Damage-Dependent miR-150-FNDC5/Pyroptosis Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jyotirmaya Behera,Jessica Ison,Michael J Voor,Neetu Tyagi

Pharmaceutical biology 60:1106-1115 PubMed35639355

2022

Myostain is involved in ginsenoside Rb1-mediated anti-obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Shi Li,Jiang-Ying Kuang,Gui-Jun Liu,Wei-Jie Wu,Xian-Lun Yin,Hao-Dong Li,Lei Wang,Tao Qin,Wen-Cheng Zhang,Yuan-Yuan Sun

Pharmaceutics 14: PubMed35745732

2022

Pentamethylquercetin Regulates Lipid Metabolism by Modulating Skeletal Muscle-Adipose Tissue Crosstalk in Obese Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jianzhao Wu,Jingxia Du,Zhi Li,Wei He,Min Wang,Manwen Jin,Lei Yang,Hui Liu

Nutrients 13: PubMed34206655

2021

Anti-Atherogenic Effect of 10% Supplementation of Anchovy () Waste Protein Hydrolysates in ApoE-Deficient Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Maria Abbate,Francesco Macrì,Francesca Arfuso,Carmelo Iaria,Fabiano Capparucci,Carmelo Anfuso,Antonio Ieni,Luca Cicero,Giovanni Briguglio,Giovanni Lanteri

Frontiers in aging neuroscience 13:640215 PubMed33613273

2021

Irisin Contributes to Neuroprotection by Promoting Mitochondrial Biogenesis After Experimental Subarachnoid Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Tianqi Tu,Shigang Yin,Jinwei Pang,Xianhui Zhang,Lifang Zhang,Yuxuan Zhang,Yuke Xie,Kecheng Guo,Ligang Chen,Jianhua Peng,Yong Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FNDC5 antibody

Western blot - Anti-FNDC5 antibody (AB131390)

Western blot - Anti-FNDC5 antibody (AB131390)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (21)

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