AB174833

Anti-FNDC5 antibody [EPR12209]

Name: Anti-FNDC5 antibody [EPR12209] (ab174833) | Abcam
Brand: Abcam Limited
SKU: AB174833
Price: 395 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(2 Reviews)

(81 Publications)

Anti-FNDC5 antibody [EPR12209] (ab174833) is a rabbit monoclonal antibody detecting FNDC5 in Western Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications

View Alternative Names

FRCP2, FNDC5, Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2

3 Images

Lab

Western blot - Anti-FNDC5 antibody [EPR12209] (AB174833)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-FNDC5 antibody [EPR12209] (ab174833) at 1/1000 dilution

All lanes:

Human fetal muscle tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 24 kDa

false

Lab

Western blot - Anti-FNDC5 antibody [EPR12209] (AB174833)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-FNDC5 antibody [EPR12209] (ab174833) at 1/5000 dilution

All lanes:

Rat muscle tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 24 kDa

false

Lab

Western blot - Anti-FNDC5 antibody [EPR12209] (AB174833)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-FNDC5 antibody [EPR12209] (ab174833) at 1/1000 dilution

All lanes:

Mouse muscle tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 24 kDa

false

Carrier free

Anti-FNDC5 antibody [EPR12209] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12209

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-FNDC5 antibody [EPR12209] (ab174833) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of FNDC5?
Anti-FNDC5 [EPR12209] (ab174833) specifically detects a band for FNDC5 (UniProt: Q8NAU1) at a molecular weight of 24kDa.

Trusted by the scientific community
Anti-FNDC5 [EPR12209] (ab174833) was first used in a scientific publication in 2013 and has been cited over 50 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR12209] also available for your convenience: ab174833, Carrier free - ab240180

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Irisin. Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.

See full target information FNDC5

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Publications (81)

Recent publications for all applications. Explore the full list and refine your search

The European journal of neuroscience 62:e70235 PubMed40960371

2025

Electrical Stimulation-Induced Muscle Damage Alters Hippocampal BDNF Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Wirtz,Rémi Chaney,Marina Cefis,Alexandre Méloux,Yuan Wang,Stéphanie Lemaire,Aurore Quirié,Julien Delezie,Gilles Gouspillou,Anne Prigent-Tessier,Philippe Garnier

Frontiers in physiology 16:1639174 PubMed40800728

2025

Comparison of passive and active motion: effect of myokine irisin on cartilage in knee osteoarthritis rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengjiao Duan,Hongpan Gao,Xue Xiao,BenXiang He

Journal of neuroinflammation 22:168 PubMed40581647

2025

Exercise-induced irisin ameliorates cognitive impairment following chronic cerebral hypoperfusion by suppressing neuroinflammation and hippocampal neuronal apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Weiping Xiao,Yibing Yang,Lu Bai,Peixuan Yang,Runze Li,Daizhi Yang,Fanying Li,Lingzhi Quan,Qiupeng Liang,Yan Yan,Tiewei Qi,Feng Liang

Endocrine connections 14: PubMed40397548

2025

lncRNA RP11-34D15.2 sponges miR-223 to promote the PGC-1α/irisin signaling pathway, contributing to increased FFA and insulin resistance in obese children.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Guo,Mengnan Lu,Yuesheng Liu,Hongai Zhang,Biyao Lian,Yanfeng Xiao,Chunyan Yin

JCI insight 10: PubMed39883525

2025

Aerobic exercise prevents renal osteodystrophy via irisin-activated osteoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wu,Huilan Li,Xiaoting Sun,Rongrong Zhong,Linli Cai,Ruibo Chen,Madiya Madeniyet,Kana Ren,Zhen Peng,Yujie Yang,Weiqin Chen,Yanling Tu,Miaoxin Lai,Jinxiu Deng,Yuting Wu,Shumin Zhao,Qingyan Ruan,Mei Rao,Sisi Xie,Ying Ye,Jianxin Wan

Journal of ovarian research 18:7 PubMed39819643

2025

Woxuanzhongzhou formula improves DHEAS and high-fat diet-induced IR and anovulatory mice via AMPK/PGC1- α/Irisin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haijuan Liu,Guohua Wang,Conglu Sui,Yanan Guo,Xiangyu He

CytoJournal 21:54 PubMed39737132

2024

Fibronectin type III domain containing protein 5/irisin alleviated sepsis-induced acute kidney injury by abating ferroptosis through the adenosine 5'-monophosphate-activated protein kinase/nuclear factor erythroid-2-related factor 2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shenghao Gui,Chaochao Zhu,Yunfeng Lu

Scientific reports 14:26881 PubMed39505986

2024

FNDC5 affects invasion and migration of oral cancer by inhibiting PI3K/Akt/Snail signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Zhao,Dongyang Xu,Xiumei Wang,Xiaofeng Wang

Bioscience trends 18:465-475 PubMed39414463

2024

FNDC5/Irisin mitigates high glucose-induced neurotoxicity in HT22 cell via ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Yang,Xiaohan Zhou,Tian Heng,Yinghai Zhu,Lihuan Gong,Na Liu,Xiuqing Yao,Yaxi Luo

Journal of sport and health science 14:100968 PubMed39187065

2024

BMAL1/PGC1α4-FNDC5/irisin axis impacts distinct outcomes of time-of-day resistance exercise.

Applications

Unspecified application

Species

Unspecified reactive species

Mingwei Guo,Fei Shen,Xiaozhen Guo,Jun Zhang,Ying Ma,Xia Wu,Hui Zuo,Jing Yao,Yepeng Hu,Dongmei Wang,Yu Li,Jin Li,Jin Qiu,Jian Yu,Meiyao Meng,Ying Zheng,Xin Chen,Mingkai Gong,Kailin Liu,Ling Jin,Xiangyu Ren,Qiang Zhang,Yu Zhao,Xuejiang Gu,Feixia Shen,Dali Li,Liangcai Gao,Chang Liu,Fei Zhou,Mian Li,Jiqiu Wang,Shuzhe Ding,Xinran Ma,Jian Lu,Cen Xie,Junjie Xiao,Lingyan Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FNDC5 antibody [EPR12209]

Western blot - Anti-FNDC5 antibody [EPR12209] (AB174833)

Western blot - Anti-FNDC5 antibody [EPR12209] (AB174833)

Western blot - Anti-FNDC5 antibody [EPR12209] (AB174833)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (81)

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