AB240180

Anti-FNDC5 antibody [EPR12209] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal FNDC5 antibody. Carrier free. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 1 publication.

View Alternative Names

FRCP2, FNDC5, Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2

3 Images

Lab

Western blot - Anti-FNDC5 antibody [EPR12209] - BSA and Azide free (AB240180)

This data was developed using ab174833, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FNDC5 antibody [EPR12209] (<a href='/en-us/products/primary-antibodies/fndc5-antibody-epr12209-ab174833'>ab174833</a>) at 1/1000 dilution

All lanes:

Human fetal muscle tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 24 kDa

false

Lab

Western blot - Anti-FNDC5 antibody [EPR12209] - BSA and Azide free (AB240180)

This data was developed using ab174833, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FNDC5 antibody [EPR12209] (<a href='/en-us/products/primary-antibodies/fndc5-antibody-epr12209-ab174833'>ab174833</a>) at 1/5000 dilution

All lanes:

Rat muscle tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 24 kDa

false

Lab

Western blot - Anti-FNDC5 antibody [EPR12209] - BSA and Azide free (AB240180)

This data was developed using ab174833, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FNDC5 antibody [EPR12209] (<a href='/en-us/products/primary-antibodies/fndc5-antibody-epr12209-ab174833'>ab174833</a>) at 1/1000 dilution

All lanes:

Mouse muscle tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 24 kDa

false

Unconjugated

Anti-FNDC5 antibody [EPR12209]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12209

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab240180 is the carrier-free version of ab174833.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Irisin. Mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA.

See full target information FNDC5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 187:4193-4212.e24 PubMed38942014

2024

Microglial-derived C1q integrates into neuronal ribonucleoprotein complexes and impacts protein homeostasis in the aging brain.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole Scott-Hewitt,Matthew Mahoney,Youtong Huang,Nils Korte,T Yvanka de Soysa,Daniel K Wilton,Emily Knorr,Kevin Mastro,Allison Chang,Allison Zhang,David Melville,Monica Schenone,Christina Hartigan,Beth Stevens

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FNDC5 antibody [EPR12209] - BSA and Azide free

Western blot - Anti-FNDC5 antibody [EPR12209] - BSA and Azide free (AB240180)

Western blot - Anti-FNDC5 antibody [EPR12209] - BSA and Azide free (AB240180)

Western blot - Anti-FNDC5 antibody [EPR12209] - BSA and Azide free (AB240180)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise