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Rabbit Recombinant Monoclonal FNDC5 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-FNDC5 Antibody [EPR26753-28] - BSA and Azide free (Detector) (AB322510), expandable thumbnail
  • Sandwich ELISA - Anti-FNDC5 Antibody [EPR26753-28] - BSA and Azide free (Detector) (AB322510), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Irisin. Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.

Alternative names

FRCP2, FNDC5, Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2

Rabbit Recombinant Monoclonal FNDC5 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26753-28
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab322510 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human FNDC5. The recommended pair for sandwich ELISA is:


Capture: Anti-FNDC5 Antibody [EPR26753-1] - BSA and Azide free (Capture) ab322509, Human FNDC5 Capture Antibody (unconjugated)


Detector: ab322510, Human FNDC5 Detector Antibody (unconjugated)


The reference range value is 24-390 pg/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Anti-FNDC5 Antibody [EPR26753-28] - BSA and Azide free (Detector) (ab322510), expandable thumbnail

    Sandwich ELISA - Anti-FNDC5 Antibody [EPR26753-28] - BSA and Azide free (Detector) (ab322510)

    Interpolated concentration of FNDC5 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: neat serum and plasma (EDTA). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% Bovine Serum Albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-FNDC5 Antibody [EPR26753-28] - BSA and Azide free (Detector) (ab322510), expandable thumbnail

    Sandwich ELISA - Anti-FNDC5 Antibody [EPR26753-28] - BSA and Azide free (Detector) (ab322510)

    Sandwich ELISA of ab322516 using human FNDC5 immunogen protein standard curve with the capture antibody Anti-FNDC5 Antibody [EPR26753-1] - BSA and Azide free (Capture) ab322509 at 2 μg/mL and detector antibody ab322510 at 0.5 μg/mL. Background-subtracted absorbance data values (mean +/- standard deviation) are displayed.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com