AB109748

Anti-FNTB antibody [EPR4706]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal FNTB antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication.

View Alternative Names

Protein farnesyltransferase subunit beta, FTase-beta, CAAX farnesyltransferase subunit beta, Ras proteins prenyltransferase subunit beta, FNTB

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-FNTB antibody [EPR4706] (AB109748)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-FNTB antibody [EPR4706] (AB109748)

Immunocytochemistry/Immunofluorescence analysis of K562 cells labelling FNTB with purified ab109748 at 1/500. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077 an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291 a mouse anti-tubulin (1/1000) and ab150120 an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1 : primary antibody (1/500) and secondary antibody ab150120 an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2 : ab7291 (1/1000) and secondary antibody ab150077 an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).

Lab

Western blot - Anti-FNTB antibody [EPR4706] (AB109748)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FNTB antibody [EPR4706] (ab109748) at 1/5000 dilution

Lane 1:

JAR whole cell lysate at 20 µg

Lane 2:

HepG2 whole cell lysate at 20 µg

Lane 3:

K562 whole cell lysate at 20 µg

Lane 4:

SH-SY5Y whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 49 kDa,66 kDa

Observed band size: 46 kDa,65 kDa

false

Unknown

Western blot - Anti-FNTB antibody [EPR4706] (AB109748)

All lanes:

Western blot - Anti-FNTB antibody [EPR4706] (ab109748) at 1/1000 dilution

Lane 1:

JAR cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Predicted band size: 49 kDa

false

Lab

Western blot - Anti-FNTB antibody [EPR4706] (AB109748)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FNTB antibody [EPR4706] (ab109748) at 1/5000 dilution

All lanes:

C6 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 49 kDa

Observed band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4706

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "For unpurified use at 1/50 - 1/100." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential subunit of the farnesyltransferase complex. Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X.

See full target information Protein farnesyltransferase subunit beta

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 16:9593-9600 PubMed29039598

2017

Expression of key enzymes in the mevalonate pathway are altered in monocrotaline-induced pulmonary arterial hypertension in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Jiang,Yu Chen,Yuxiang Zhu,Guosheng Fu,Shiming Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FNTB antibody [EPR4706]

Immunocytochemistry/ Immunofluorescence - Anti-FNTB antibody [EPR4706] (AB109748)

Western blot - Anti-FNTB antibody [EPR4706] (AB109748)

Western blot - Anti-FNTB antibody [EPR4706] (AB109748)

Western blot - Anti-FNTB antibody [EPR4706] (AB109748)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise