Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)

Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543) is a rabbit monoclonal antibody detecting Folate Binding Protein/FBP in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (AB221543), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P
Human	Tested	Tested
Mouse	Tested	Tested
Rat	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000 - 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000 - 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

See full target information FOLR1

Function

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells (PubMed:19074442, PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728). Has high affinity for folate and folic acid analogs at neutral pH (PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728). Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release (PubMed:8567728). Required for normal embryonic development and normal cell proliferation (By similarity).

Alternative names

FOLR, FOLR1, Folate receptor alpha, FR-alpha, Adult folate-binding protein, Folate receptor 1, KB cells FBP, Ovarian tumor-associated antigen MOv18, FBP

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR20277
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of Folate Binding Protein/FBP?

Anti-Folate Binding Protein/FBP [EPR20277] (ab221543) specifically detects a band for Folate Binding Protein/FBP (UniProt: P15328) at a molecular weight of 30kDa.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed

The specificity of Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543) has been confirmed by Western blot testing in FOLR1 Knockout HeLa cell line, ab264921.

Other related products

We have a range of other formats of antibody clone [EPR20277] also available for your convenience:
ab221543, Carrier free - ab235140, Alexa Fluor® 555 - ab309247, Alexa Fluor® 647 - ab309500

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Folate Binding Protein (FBP) also known as FolR1 protein or FOLR-1 is a significant protein with a mass of about 32-38 kDa. It binds folate with high affinity and facilitates its transport into the cell. FBP can be found in various tissues with notable expression in the placenta kidney and certain epithelial tissues. The protein mediates folate uptake by capturing folates in the extracellular matrix and channeling them through receptor-mediated endocytosis.

Biological function summary

FBP plays a critical role in cellular growth and replication by ensuring sufficient folate supply. It belongs to a family of receptors not forming part of a larger protein complex. FBP's primary function is delivering folate an important B vitamin which acts as a one-carbon donor in DNA synthesis and repair. Optimal folate transport by FBP supports cellular functions particularly in rapidly dividing cells.

Pathways

FBP significantly contributes to the folate-mediated one-carbon metabolic pathway critical for nucleotide biosynthesis. This pathway interconnects with the homocysteine remethylation cycle. FBP associates with other enzymes such as dihydrofolate reductase (DHFR) and methylenetetrahydrofolate reductase (MTHFR) which also play key roles in these pathways. These interactions ensure efficient folate metabolism and maintenance of normal cellular functions.

Associated diseases and disorders

FBP's relation to cancer and neural tube defects is well documented. Overexpression of FBP has been observed in various cancers including ovarian and breast cancer which indicates its potential role in tumor progression. In neural tube defects altered folate transport due to variations in FBP expression impacts neural development. FBP connects to other markers like HER2 in cancer which may form part of a therapeutic target strategy.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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10 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051, ready to use). Cytoplasmic staining on human kidney (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051, ready to use).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)
Lanes 1-4: Merged signal (red and green). Green - ab221543 observed at 36, 38 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 52 kDa.
ab221543 Anti-Folate Binding Protein/FBP antibody [EPR20277] was shown to specifically react with Folate Binding Protein/FBP in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human FOLR1 (Folate Binding Protein/FBP) knockout HeLa cell line ab264921 (knockout cell lysate Human FOLR1 (Folate Binding Protein/FBP) knockout HeLa cell lysate ab257270) was used. Wild-type and Folate Binding Protein/FBP knockout samples were subjected to SDS-PAGE. ab221543 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
The molecular weight (36,38kDa) observed were knockout validated and consistent with what has been described in the literature (PMID: 23144806).
All lanes: Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: Western blot - Human FOLR1 (Folate Binding Protein/FBP) knockout HeLa cell line (Human FOLR1 (Folate Binding Protein/FBP) knockout HeLa cell line ab264921)
Lane 2: FOLR1 knockout HeLa cell lysate at 20 µg
Lane 3: JAR cell lysate at 20 µg
Lane 4: MDA-MB-231 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 38 kDa, 36 kDa
Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)
Exposure time:
Lanes 1-2: 5 seconds
The molecular weight (36,38kDa) observed were knockout validated and consistent with what has been described in the literature (PMID: 23144806).
All lanes: Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543) at 1/5000 dilution
Lane 1: GST-His-tagged human recombinant FOLR1 (aa 25-160), 20 ng
Lane 2: His-tagged human recombinant FOLR2 (aa 24-154), 20 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 38 kDa, 36 kDa
Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)
Exposure times:
Lane 1:10 seconds
Lane 2: 30 seconds
Lanes 3-4: 5 seconds
Lane 5: 3 minutes
The molecular weight (36,38kDa) observed were knockout validated and consistent with what has been described in the literature (PMID: 23144806).
All lanes: Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543) at 1/1000 dilution
Lane 1: JAR (human placenta choriocarcinoma epithelial cells) whole cell lysate at 20 µg
Lane 2: Human fetal kidney lysate at 20 µg
Lane 3: Mouse kidney lysate at 20 µg
Lane 4: Rat kidney lysate
Lane 5: HeLa (Human cervix adenocarcinoma epithelial cells) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 38 kDa, 36 kDa
Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)
Lanes 1-4: Merged signal (red and green). Green - ab221543 observed at 36, 38 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 52 kDa.
ab221543 Anti-Folate Binding Protein/FBP antibody [EPR20277] was shown to specifically react with Folate Binding Protein/FBP in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human FOLR1 (Folate Binding Protein/FBP) knockout HeLa cell line ab264921 (knockout cell lysate Human FOLR1 (Folate Binding Protein/FBP) knockout HeLa cell lysate ab257270) was used. Wild-type and Folate Binding Protein/FBP knockout samples were subjected to SDS-PAGE. ab221543 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
The molecular weight (36,38kDa) observed were knockout validated and consistent with what has been described in the literature (PMID: 23144806).
All lanes: Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: FOLR1 knockout HeLa cell lysate at 20 µg
Lane 3: JAR cell lysate at 20 µg
Lane 4: MDA-MB-231 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 38 kDa, 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Anti-GNAT2 antibody ab97501, ready to use). Mainly membranous staining on tumor cells of human ovarian cancer (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Anti-GNAT2 antibody ab97501, ready to use).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Anti-GNAT2 antibody ab97501, ready to use). Cytoplasmic staining on tumor cells of human lung cancer (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Anti-GNAT2 antibody ab97501, ready to use).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Anti-GNAT2 antibody ab97501, ready to use). Positive staining on mouse kidney (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Anti-GNAT2 antibody ab97501, ready to use).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Anti-GNAT2 antibody ab97501, ready to use). Positive staining on rat kidney (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Anti-GNAT2 antibody ab97501, ready to use).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)
Negative control:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Anti-GNAT2 antibody ab97501, ready to use). No staining on human tonsil (PMID: 22547449). Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.