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AB325147

Anti-Folate Receptor 4 antibody [EPR30390-526] - BSA and Azide free

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Rabbit Recombinant Monoclonal Folate Receptor 4 antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

Folbp3, Folr4, Juno, Izumo1r, Sperm-egg fusion protein Juno, Folate receptor 4, Folate receptor delta, Folate-binding protein 3, IZUMO1 receptor protein JUNO, FR-delta

2 Images
Flow Cytometry - Anti-Folate Receptor 4 antibody [EPR30390-526] - BSA and Azide free (AB325147)
  • Flow Cyt

Lab

Flow Cytometry - Anti-Folate Receptor 4 antibody [EPR30390-526] - BSA and Azide free (AB325147)

This data was developed using ab325135, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cells labelling Folate Receptor 4 with ab325135 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with CD8a conjugated to Brilliant Violet 421.

Flow Cytometry - Anti-Folate Receptor 4 antibody [EPR30390-526] - BSA and Azide free (AB325147)
  • Flow Cyt

Lab

Flow Cytometry - Anti-Folate Receptor 4 antibody [EPR30390-526] - BSA and Azide free (AB325147)

This data was developed using ab325135, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cells labelling Folate Receptor 4 with ab325135 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with CD4 conjugated to Alexa Fluor®647.

  • Unconjugated

    Anti-Folate Receptor 4 antibody [EPR30390-526]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30390-526

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab325147 is the carrier-free version of ab325135.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for species-specific gamete recognition and fertilization (PubMed : 24739963, PubMed : 26859261, PubMed : 27309808, PubMed : 27416963). The IZUMO1 : IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion (PubMed : 24739963, PubMed : 26859261, PubMed : 27309808). The ligand-receptor interaction probably does not act as a membrane 'fusogen' (PubMed : 24739963, PubMed : 26859261, PubMed : 27309808). Does not bind folate (PubMed : 24739963).
See full target information Izumo1r
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com