AB203131

Anti-Follistatin antibody

Name: Anti-Follistatin antibody (ab203131) | Abcam
Brand: Abcam Limited
SKU: AB203131
Rating: 1 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal Follistatin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FST aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Follistatin, FS, Activin-binding protein, FST

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Follistatin antibody (AB203131)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Follistatin antibody (AB203131)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling Follistatin with ab203131 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human FST aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P19883

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>(or 1/50 - 1/200 using a fluorescent secondary antibody).</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Follistatin also known as FS or FST is a glycoprotein with an approximate mass of 32 kDa. This protein is part of the follistatin family and plays an important role in various physiological mechanisms. Follistatin synthesizes and secretes in different tissues including the liver gonads and skeletal muscles. It binds to and inhibits members of the transforming growth factor-beta (TGF-β) superfamily particularly activin. This binding prevents activin from interacting with its receptors.

Biological function summary

Follistatin modulates multiple processes such as cell growth differentiation and reproductive physiology. It acts as a binding protein for activin neutralizing its activity. By preventing activin from engaging with its receptor complexes follistatin influences developmental and regenerative processes. Despite not being a part of a transmembrane complex its interaction with activin impacts extracellular interactions.

Pathways

Follistatin plays a significant role in the regulation of the TGF-β signaling pathway and the muscle growth pathway. In the TGF-β pathway it counteracts the signaling of activin which influences cellular homeostasis and inflammatory responses. In the muscle growth pathway follistatin interferes with myostatin another member of the TGF-β family promoting muscle growth. This cross-talk with proteins such as myostatin demonstrates follistatin's influence in muscle regeneration and development.

Follistatin has clear associations with muscle-related and reproductive disorders. It is linked to muscle wasting diseases such as muscular dystrophy due to its inhibitory role on myostatin known to restrain muscle growth. Follistatin abnormalities can also contribute to conditions like polycystic ovary syndrome (PCOS) due to its regulation of reproductive processes. These ties display follistatin's vital interactions with proteins like activin and myostatin highlighting its importance in disease pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multifunctional regulatory protein whose primary function is to antagonize members of the transforming growth factor beta (TGF-beta) superfamily including activin, myostatin, GDF11 or bone morphogenetic proteins (BMPs) (PubMed : 11279126, PubMed : 16482217, PubMed : 18535106). Mechanistically, binds to these ligands in the extracellular space, blocking their type II receptor-binding site to inhibit downstream signaling (PubMed : 16482217). Plays an essential role in muscle fiber formation and growth both by preventing the repressive effects of myostatin and through SMAD3/AKT/mTOR signaling independently of myostatin (By similarity). Also promotes neural differentiation by antagonizing the action BMP4 (By similarity). Acts as a specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH) by sequestering activin A/INHBA (PubMed : 11279126). On the other hand, translocates into the nucleus where it down-regulates rRNA synthesis and ribosome biogenesis to maintain cellular energy homeostasis by binding to rDNA.

See full target information FST

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Viruses 15: PubMed37243119

2023

Immunostaining of βA-Activin and Follistatin Is Decreased in HPV(+) Cervical Pre-Neoplastic and Neoplastic Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Victor Jesus Huaringa Payano,Lara Verônica de Araújo Lopes,Larissa Rodrigues Peixoto,Keila Alves da Silva,Tania Maria Ortiga-Carvalho,Alexandre Tafuri,Annamaria Ravara Vago,Enrrico Bloise

Nature communications 9:2759 PubMed30018341

2018

Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Bruce A Rheaume,Amyeo Jereen,Mohan Bolisetty,Muhammad S Sajid,Yue Yang,Kathleen Renna,Lili Sun,Paul Robson,Ephraim F Trakhtenberg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Follistatin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Follistatin antibody (AB203131)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise