Rabbit Polyclonal Follistatin antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 7 publications.
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- Clinical cancer research : an official journal of the American Association for Cancer Research 29:1969-19832023Quiescent Ovarian Cancer Cells Secrete Follistatin to Induce Chemotherapy Resistance in Surrounding Cells in Response to Chemotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander J Cole,Santiago Panesso-Gómez,Jaynish S Shah,Tonge Ebai,Qi Jiang,Ece Gumusoglu-Acar,Maya G Bello,Anda Vlad,Francesmary Modugno,Robert P Edwards,Ronald J BuckanovichPubMed 36795892
- Physiology international 107:220-2302020High-intensity interval training-induced hypertrophy in gastrocnemius muscle via improved IGF-I/Akt/FoxO and myostatin/Smad signaling pathways in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoheil Biglari,Alireza Ghardashi Afousi,Farnoosh Mafi,Fatemeh ShabkhizPubMed 32644938
- Drug testing and analysis 11:1675-16972019Detection of black market follistatin 344.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristian Reichel,Günter Gmeiner,Mario ThevisPubMed 31758732
- PLoS genetics 15:e10083682019Telomere dysfunction impairs epidermal stem cell specification and differentiation by disrupting BMP/pSmad/P63 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Liu et. al.PubMed 31518356
- Diabetes, metabolic syndrome and obesity : targets 11:65-842018Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRajan Singh et. al.PubMed 29618935
- Investigative ophthalmology & visual science 53:7358-692012The effects of transforming growth factor-β2 on the expression of follistatin and activin A in normal and glaucomatous human trabecular meshwork cells and tissues.Applications:WBReactive species:Unspecified reactive speciesAshley M Fitzgerald,Cecilia Benz,Abbot F Clark,Robert J WordingerPubMed 23010638
- Endocrinology 152:2976-8620111,25(OH)2vitamin D3 stimulates myogenic differentiation by inhibiting cell proliferation and modulating the expression of promyogenic growth factors and myostatin in C2C12 skeletal muscle cells.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| Chicken | Predicted |
| Chimpanzee | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Pig | Predicted |
| Rhesus monkey | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Chicken | Dilution info - | Notes - |
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Target data
Function
Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
Alternative names
Follistatin, FS, Activin-binding protein, FST
Recommended products
Rabbit Polyclonal Follistatin antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
ab64490 is specific for the FST315 isoform. It does not bind the FST288 isoform.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Follistatin also known as FS or FST is a glycoprotein with an approximate mass of 32 kDa. This protein is part of the follistatin family and plays an important role in various physiological mechanisms. Follistatin synthesizes and secretes in different tissues including the liver gonads and skeletal muscles. It binds to and inhibits members of the transforming growth factor-beta (TGF-β) superfamily particularly activin. This binding prevents activin from interacting with its receptors.
- Biological function summary
Follistatin modulates multiple processes such as cell growth differentiation and reproductive physiology. It acts as a binding protein for activin neutralizing its activity. By preventing activin from engaging with its receptor complexes follistatin influences developmental and regenerative processes. Despite not being a part of a transmembrane complex its interaction with activin impacts extracellular interactions.
- Pathways
Follistatin plays a significant role in the regulation of the TGF-β signaling pathway and the muscle growth pathway. In the TGF-β pathway it counteracts the signaling of activin which influences cellular homeostasis and inflammatory responses. In the muscle growth pathway follistatin interferes with myostatin another member of the TGF-β family promoting muscle growth. This cross-talk with proteins such as myostatin demonstrates follistatin's influence in muscle regeneration and development.
- Associated diseases and disorders
Follistatin has clear associations with muscle-related and reproductive disorders. It is linked to muscle wasting diseases such as muscular dystrophy due to its inhibitory role on myostatin known to restrain muscle growth. Follistatin abnormalities can also contribute to conditions like polycystic ovary syndrome (PCOS) due to its regulation of reproductive processes. These ties display follistatin's vital interactions with proteins like activin and myostatin highlighting its importance in disease pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Follistatin antibody (ab64490)
Blocking buffer: 2% BSA
Gel type: MOPS
All lanes: Western blot - Anti-Follistatin antibody (ab64490) at 1 µg/mL
Lane 1: Mouse liver lysate at 20 µg
Lane 2: Rat liver lysate at 20 µg
Lane 3: Human liver lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Predicted band size: 38 kDa
Observed band size: 15 kDa, 35 kDa, 38 kDa, 60 kDa, 70 kDa
Exposure time: 2min
Western blot - Anti-Follistatin antibody (ab64490)
All lanes: Western blot - Anti-Follistatin antibody (ab64490) at 1 µg/mL
Lane 1: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Liver (Mouse) Tissue Lysate at 10 µg
Lane 3: Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg
Lane 4: MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg
Lane 5: Colon (Mouse) Tissue Lysate at 10 µg
Lane 6: Liver (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 15 kDa, 38 kDa, 75 kDa
Exposure time: 1min
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