AB64490

Anti-Follistatin antibody

Name: Anti-Follistatin antibody (ab64490) | Abcam
Brand: Abcam Limited
SKU: AB64490
Rating: 3 (3 reviews)

(3 Reviews)

(7 Publications)

Rabbit Polyclonal Follistatin antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

Follistatin, FS, Activin-binding protein, FST

2 Images

Supplier Data

Western blot - Anti-Follistatin antibody (AB64490)

Blocking buffer : 2% BSA

Gel type : MOPS

All lanes:

Western blot - Anti-Follistatin antibody (ab64490) at 1 µg/mL

Lane 1:

Mouse liver lysate at 20 µg

Lane 2:

Rat liver lysate at 20 µg

Lane 3:

Human liver lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

Predicted band size: 38 kDa

Observed band size: 15 kDa,35 kDa,38 kDa,60 kDa,70 kDa

false

Exposure time: 2min

Project

Western blot - Anti-Follistatin antibody (AB64490)

All lanes:

Western blot - Anti-Follistatin antibody (ab64490) at 1 µg/mL

Lane 1:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Liver (Mouse) Tissue Lysate at 10 µg

Lane 3:

Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg

Lane 5:

Colon (Mouse) Tissue Lysate at 10 µg

Lane 6:

Liver (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 38 kDa

Observed band size: 15 kDa,38 kDa,75 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab64490 is specific for the FST315 isoform. It does not bind the FST288 isoform.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Follistatin also known as FS or FST is a glycoprotein with an approximate mass of 32 kDa. This protein is part of the follistatin family and plays an important role in various physiological mechanisms. Follistatin synthesizes and secretes in different tissues including the liver gonads and skeletal muscles. It binds to and inhibits members of the transforming growth factor-beta (TGF-β) superfamily particularly activin. This binding prevents activin from interacting with its receptors.

Biological function summary

Follistatin modulates multiple processes such as cell growth differentiation and reproductive physiology. It acts as a binding protein for activin neutralizing its activity. By preventing activin from engaging with its receptor complexes follistatin influences developmental and regenerative processes. Despite not being a part of a transmembrane complex its interaction with activin impacts extracellular interactions.

Pathways

Follistatin plays a significant role in the regulation of the TGF-β signaling pathway and the muscle growth pathway. In the TGF-β pathway it counteracts the signaling of activin which influences cellular homeostasis and inflammatory responses. In the muscle growth pathway follistatin interferes with myostatin another member of the TGF-β family promoting muscle growth. This cross-talk with proteins such as myostatin demonstrates follistatin's influence in muscle regeneration and development.

Follistatin has clear associations with muscle-related and reproductive disorders. It is linked to muscle wasting diseases such as muscular dystrophy due to its inhibitory role on myostatin known to restrain muscle growth. Follistatin abnormalities can also contribute to conditions like polycystic ovary syndrome (PCOS) due to its regulation of reproductive processes. These ties display follistatin's vital interactions with proteins like activin and myostatin highlighting its importance in disease pathways.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Multifunctional regulatory protein whose primary function is to antagonize members of the transforming growth factor beta (TGF-beta) superfamily including activin, myostatin, GDF11 or bone morphogenetic proteins (BMPs) (PubMed : 11279126, PubMed : 16482217, PubMed : 18535106). Mechanistically, binds to these ligands in the extracellular space, blocking their type II receptor-binding site to inhibit downstream signaling (PubMed : 16482217). Plays an essential role in muscle fiber formation and growth both by preventing the repressive effects of myostatin and through SMAD3/AKT/mTOR signaling independently of myostatin (By similarity). Also promotes neural differentiation by antagonizing the action BMP4 (By similarity). Acts as a specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH) by sequestering activin A/INHBA (PubMed : 11279126). On the other hand, translocates into the nucleus where it down-regulates rRNA synthesis and ribosome biogenesis to maintain cellular energy homeostasis by binding to rDNA.

See full target information FST

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 29:1969-1983 PubMed36795892

2023

Quiescent Ovarian Cancer Cells Secrete Follistatin to Induce Chemotherapy Resistance in Surrounding Cells in Response to Chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander J Cole,Santiago Panesso-Gómez,Jaynish S Shah,Tonge Ebai,Qi Jiang,Ece Gumusoglu-Acar,Maya G Bello,Anda Vlad,Francesmary Modugno,Robert P Edwards,Ronald J Buckanovich

Physiology international 107:220-230 PubMed32644938

2020

High-intensity interval training-induced hypertrophy in gastrocnemius muscle via improved IGF-I/Akt/FoxO and myostatin/Smad signaling pathways in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Soheil Biglari,Alireza Ghardashi Afousi,Farnoosh Mafi,Fatemeh Shabkhiz

Drug testing and analysis 11:1675-1697 PubMed31758732

2019

Detection of black market follistatin 344.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Reichel,Günter Gmeiner,Mario Thevis

PLoS genetics 15:e1008368 PubMed31518356

2019

Telomere dysfunction impairs epidermal stem cell specification and differentiation by disrupting BMP/pSmad/P63 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Na Liu,Yu Yin,Haiying Wang,Zhongcheng Zhou,Xiaoyan Sheng,Haifeng Fu,Renpeng Guo,Hua Wang,Jiao Yang,Peng Gong,Wen Ning,Zhenyu Ju,Yifei Liu,Lin Liu

Diabetes, metabolic syndrome and obesity : targets 11:65-84 PubMed29618935

2018

Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Rajan Singh,Shehla Pervin,Se-Jin Lee,Alan Kuo,Victor Grijalva,John David,Laurent Vergnes,Srinivasa T Reddy

Investigative ophthalmology & visual science 53:7358-69 PubMed23010638

2012

The effects of transforming growth factor-β2 on the expression of follistatin and activin A in normal and glaucomatous human trabecular meshwork cells and tissues.

Applications

Species

Unspecified reactive species

Ashley M Fitzgerald,Cecilia Benz,Abbot F Clark,Robert J Wordinger

Endocrinology 152:2976-86 PubMed21673099

2011

1,25(OH)2vitamin D3 stimulates myogenic differentiation by inhibiting cell proliferation and modulating the expression of promyogenic growth factors and myostatin in C2C12 skeletal muscle cells.

Applications

Species

Mouse

Leah A Garcia,Keisha K King,Monica G Ferrini,Keith C Norris,Jorge N Artaza

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Follistatin antibody

Western blot - Anti-Follistatin antibody (AB64490)

Western blot - Anti-Follistatin antibody (AB64490)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise