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AB157471

Anti-Follistatin antibody [EPR10903]

4

(1 Review)

|

(6 Publications)

Rabbit Recombinant Monoclonal Follistatin antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 6 publications.

View Alternative Names

Follistatin, FS, Activin-binding protein, FST

1 Images
Western blot - Anti-Follistatin antibody [EPR10903] (AB157471)
  • WB

Unknown

Western blot - Anti-Follistatin antibody [EPR10903] (AB157471)

Blocking/Diluting buffer : 5% NFDM/TBST.

We are unsure how to define the extra bands.

All lanes:

Western blot - Anti-Follistatin antibody [EPR10903] (ab157471) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Human heart lysates at 20 µg

Lane 3:

Rat heart lysates at 20 µg

Lane 4:

Rat kidney lysates at 20 µg

Lane 5:

C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 38 kDa

Observed band size: 38 kDa

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  • Carrier free

    Anti-Follistatin antibody [EPR10903] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10903

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Follistatin also known as FS or FST is a glycoprotein with an approximate mass of 32 kDa. This protein is part of the follistatin family and plays an important role in various physiological mechanisms. Follistatin synthesizes and secretes in different tissues including the liver gonads and skeletal muscles. It binds to and inhibits members of the transforming growth factor-beta (TGF-β) superfamily particularly activin. This binding prevents activin from interacting with its receptors.
Biological function summary

Follistatin modulates multiple processes such as cell growth differentiation and reproductive physiology. It acts as a binding protein for activin neutralizing its activity. By preventing activin from engaging with its receptor complexes follistatin influences developmental and regenerative processes. Despite not being a part of a transmembrane complex its interaction with activin impacts extracellular interactions.

Pathways

Follistatin plays a significant role in the regulation of the TGF-β signaling pathway and the muscle growth pathway. In the TGF-β pathway it counteracts the signaling of activin which influences cellular homeostasis and inflammatory responses. In the muscle growth pathway follistatin interferes with myostatin another member of the TGF-β family promoting muscle growth. This cross-talk with proteins such as myostatin demonstrates follistatin's influence in muscle regeneration and development.

Follistatin has clear associations with muscle-related and reproductive disorders. It is linked to muscle wasting diseases such as muscular dystrophy due to its inhibitory role on myostatin known to restrain muscle growth. Follistatin abnormalities can also contribute to conditions like polycystic ovary syndrome (PCOS) due to its regulation of reproductive processes. These ties display follistatin's vital interactions with proteins like activin and myostatin highlighting its importance in disease pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
See full target information FST
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular oncology 17:469-486 PubMed36608258

2023

Integrative characterisation of secreted factors involved in intercellular communication between prostate epithelial or cancer cells and fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yunjian Wu,Kimberley C Clark,Birunthi Niranjan,Anderly C Chüeh,Lisa G Horvath,Renea A Taylor,Roger J Daly

Cancers 15: PubMed36612143

2022

Activin B and Activin C Have Opposing Effects on Prostate Cancer Progression and Cell Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Karen L Reader,Simon John-McHaffie,Sylvia Zellhuber-McMillan,Tim Jowett,David G Mottershead,Heather E Cunliffe,Elspeth J Gold

American journal of cancer research 11:712-728 PubMed33791149

2021

FSTL1 promotes growth and metastasis in gastric cancer by activating AKT related pathway and predicts poor survival.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjie Wu,Yongfeng Ding,Nan Wu,Junjie Jiang,Yingying Huang,Fanyu Zhang,Haiyong Wang,Quan Zhou,Yan Yang,Wei Zhuo,Lisong Teng

Drug testing and analysis 12:1636-1648 PubMed32959984

2020

Detection of follistatin-based inhibitors of the TGF-β signaling pathways in serum/plasma by means of LC-HRMS/MS and Western blotting.

Applications

Unspecified application

Species

Unspecified reactive species

Katja Walpurgis,Tim Weigand,Andre Knoop,Andreas Thomas,Christian Reichel,Frank Dellanna,Mario Thevis

Drug testing and analysis 11:1675-1697 PubMed31758732

2019

Detection of black market follistatin 344.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Reichel,Günter Gmeiner,Mario Thevis

Oncotarget 6:14399-412 PubMed25973542

2015

The EMT-activator ZEB1 induces bone metastasis associated genes including BMP-inhibitors.

Applications

WB

Species

Unspecified reactive species

Kerstin Mock,Bogdan-Tiberius Preca,Tilman Brummer,Simone Brabletz,Marc P Stemmler,Thomas Brabletz
View all publications
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Product promise

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