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AB249335

Anti-Follistatin antibody [EPR10903] - BSA and Azide free

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Rabbit Recombinant Monoclonal Follistatin antibody. Carrier free. Suitable for WB and reacts with Human, Rat samples.

View Alternative Names

Follistatin, FS, Activin-binding protein, FST

1 Images
Western blot - Anti-Follistatin antibody [EPR10903] - BSA and Azide free (AB249335)
  • WB

Unknown

Western blot - Anti-Follistatin antibody [EPR10903] - BSA and Azide free (AB249335)

We are unsure how to define the extra bands.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157471).

All lanes:

Western blot - Anti-Follistatin antibody [EPR10903] (<a href='/en-us/products/primary-antibodies/follistatin-antibody-epr10903-ab157471'>ab157471</a>) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Human heart lysates at 20 µg

Lane 3:

Rat heart lysates at 20 µg

Lane 4:

Rat kidney lysates at 20 µg

Lane 5:

C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 38 kDa

Observed band size: 38 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10903

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249335 is the carrier-free version of ab157471.

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Follistatin also known as FS or FST is a glycoprotein with an approximate mass of 32 kDa. This protein is part of the follistatin family and plays an important role in various physiological mechanisms. Follistatin synthesizes and secretes in different tissues including the liver gonads and skeletal muscles. It binds to and inhibits members of the transforming growth factor-beta (TGF-β) superfamily particularly activin. This binding prevents activin from interacting with its receptors.
Biological function summary

Follistatin modulates multiple processes such as cell growth differentiation and reproductive physiology. It acts as a binding protein for activin neutralizing its activity. By preventing activin from engaging with its receptor complexes follistatin influences developmental and regenerative processes. Despite not being a part of a transmembrane complex its interaction with activin impacts extracellular interactions.

Pathways

Follistatin plays a significant role in the regulation of the TGF-β signaling pathway and the muscle growth pathway. In the TGF-β pathway it counteracts the signaling of activin which influences cellular homeostasis and inflammatory responses. In the muscle growth pathway follistatin interferes with myostatin another member of the TGF-β family promoting muscle growth. This cross-talk with proteins such as myostatin demonstrates follistatin's influence in muscle regeneration and development.

Follistatin has clear associations with muscle-related and reproductive disorders. It is linked to muscle wasting diseases such as muscular dystrophy due to its inhibitory role on myostatin known to restrain muscle growth. Follistatin abnormalities can also contribute to conditions like polycystic ovary syndrome (PCOS) due to its regulation of reproductive processes. These ties display follistatin's vital interactions with proteins like activin and myostatin highlighting its importance in disease pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional regulatory protein whose primary function is to antagonize members of the transforming growth factor beta (TGF-beta) superfamily including activin, myostatin, GDF11 or bone morphogenetic proteins (BMPs) (PubMed : 11279126, PubMed : 16482217, PubMed : 18535106). Mechanistically, binds to these ligands in the extracellular space, blocking their type II receptor-binding site to inhibit downstream signaling (PubMed : 16482217). Plays an essential role in muscle fiber formation and growth both by preventing the repressive effects of myostatin and through SMAD3/AKT/mTOR signaling independently of myostatin (By similarity). Also promotes neural differentiation by antagonizing the action BMP4 (By similarity). Acts as a specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH) by sequestering activin A/INHBA (PubMed : 11279126). On the other hand, translocates into the nucleus where it down-regulates rRNA synthesis and ribosome biogenesis to maintain cellular energy homeostasis by binding to rDNA.
See full target information FST
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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