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AB89556

Anti-Follistatin antibody [MM0296-9D28]

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(1 Publication)

Mouse Monoclonal Follistatin antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FST.

View Alternative Names

Follistatin, FS, Activin-binding protein, FST

1 Images
Western blot - Anti-Follistatin antibody [MM0296-9D28] (AB89556)
  • WB

Supplier Data

Western blot - Anti-Follistatin antibody [MM0296-9D28] (AB89556)

All lanes:

Western blot - Anti-Follistatin antibody [MM0296-9D28] (ab89556) at 1/200 dilution

All lanes:

Human placenta tissue lysate

Predicted band size: 38 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0296-9D28

Isotype

IgG2

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FST.

P19883

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Alternative Products

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Primary Antibodies

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Primary Antibodies

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Complementary Products

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Secondary Antibodies

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Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/100", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 µm filter.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Follistatin also known as FS or FST is a glycoprotein with an approximate mass of 32 kDa. This protein is part of the follistatin family and plays an important role in various physiological mechanisms. Follistatin synthesizes and secretes in different tissues including the liver gonads and skeletal muscles. It binds to and inhibits members of the transforming growth factor-beta (TGF-β) superfamily particularly activin. This binding prevents activin from interacting with its receptors.
Biological function summary

Follistatin modulates multiple processes such as cell growth differentiation and reproductive physiology. It acts as a binding protein for activin neutralizing its activity. By preventing activin from engaging with its receptor complexes follistatin influences developmental and regenerative processes. Despite not being a part of a transmembrane complex its interaction with activin impacts extracellular interactions.

Pathways

Follistatin plays a significant role in the regulation of the TGF-β signaling pathway and the muscle growth pathway. In the TGF-β pathway it counteracts the signaling of activin which influences cellular homeostasis and inflammatory responses. In the muscle growth pathway follistatin interferes with myostatin another member of the TGF-β family promoting muscle growth. This cross-talk with proteins such as myostatin demonstrates follistatin's influence in muscle regeneration and development.

Follistatin has clear associations with muscle-related and reproductive disorders. It is linked to muscle wasting diseases such as muscular dystrophy due to its inhibitory role on myostatin known to restrain muscle growth. Follistatin abnormalities can also contribute to conditions like polycystic ovary syndrome (PCOS) due to its regulation of reproductive processes. These ties display follistatin's vital interactions with proteins like activin and myostatin highlighting its importance in disease pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional regulatory protein whose primary function is to antagonize members of the transforming growth factor beta (TGF-beta) superfamily including activin, myostatin, GDF11 or bone morphogenetic proteins (BMPs) (PubMed : 11279126, PubMed : 16482217, PubMed : 18535106). Mechanistically, binds to these ligands in the extracellular space, blocking their type II receptor-binding site to inhibit downstream signaling (PubMed : 16482217). Plays an essential role in muscle fiber formation and growth both by preventing the repressive effects of myostatin and through SMAD3/AKT/mTOR signaling independently of myostatin (By similarity). Also promotes neural differentiation by antagonizing the action BMP4 (By similarity). Acts as a specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH) by sequestering activin A/INHBA (PubMed : 11279126). On the other hand, translocates into the nucleus where it down-regulates rRNA synthesis and ribosome biogenesis to maintain cellular energy homeostasis by binding to rDNA.
See full target information FST
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer therapeutics 21:1125-1135 PubMed35499387

2022

Luxeptinib (CG-806) Targets FLT3 and Clusters of Kinases Operative in Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

William G Rice,Stephen B Howell,Hongying Zhang,Nasrin Rastgoo,Andrea Local,Stephen E Kurtz,Pierrette Lo,Daniel Bottomly,Beth Wilmot,Shannon K McWeeney,Brian J Druker,Jeffrey W Tyner
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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