Mouse Monoclonal FOLR2 antibody. Suitable for Flow Cyt and reacts with Transfected cell line samples. Immunogen corresponding to Cell preparation containing FOLR2 protein.
Images
![Flow Cytometry - Anti-FOLR2 antibody [EM-35] (AB234190), expandable thumbnail](https://content.abcam.com/products/images/folr2-antibody-em-35-ab234190--flow-cytometry-img47140.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-FOLR2 antibody [EM-35] (AB234190)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing FOLR2 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P14207
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Mouse | Not recommended |
| Transfected cell line | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info 1-4 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release.
Alternative names
Folate receptor beta, FR-beta, Folate receptor 2, Placental folate-binding protein, FBP, FOLR2
Recommended products
Mouse Monoclonal FOLR2 antibody. Suitable for Flow Cyt and reacts with Transfected cell line samples. Immunogen corresponding to Cell preparation containing FOLR2 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing FOLR2 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P14207
- Clone number
- EM-35
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Purity > 95% (by SDS-PAGE).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Folate receptor beta also known as FOLR2 is a protein with a molecular mass of approximately 32 kDa. It facilitates the transport of folate into cells. FOLR2 binds folate with high affinity and plays an important role in cellular metabolism. The receptor is mainly expressed in the placenta and certain myeloid cells such as macrophages and monocytes. Its expression is also notable in certain cancer cells but not in most normal tissues.
- Biological function summary
Folate receptor beta plays a significant role in folate uptake contributing to DNA synthesis and repair processes. FOLR2 as a part of the cell membrane forms a complex with other molecules to capture and internalize folate from the extracellular environment. This process is essential for the proliferation and survival of cells that require large amounts of folate including developing embryos and certain immune cells.
- Pathways
The function of folate receptor beta connects with the folate-mediated one-carbon metabolism pathway critical for nucleotide biosynthesis and methylation reactions. This pathway influences the synthesis of purines and pyrimidines supporting DNA replication and repair. FOLR2 interacts with folate transport proteins and enzymes involved in the methionine cycle which interlinks with amino acid metabolism and epigenetic regulation.
- Associated diseases and disorders
Folate receptor beta is associated with rheumatoid arthritis and ovarian cancer. In rheumatoid arthritis FOLR2 expression on activated macrophages indicates its potential as a biomarker and target for therapy. The overexpression of FOLR2 in ovarian cancer makes it a target for folate-linked therapeutic agents. The relationship between FOLR2 and immune proteins in inflammatory conditions highlights its role in modulating immune responses in disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry - Anti-FOLR2 antibody [EM-35] (ab234190), expandable thumbnail](https://content.abcam.com/products/images/folr2-antibody-em-35-ab234190--flow-cytometry-img47140.jpg "Flow Cytometry - Anti-FOLR2 antibody [EM-35] (ab234190)")
Flow Cytometry - Anti-FOLR2 antibody [EM-35] (ab234190)
Flow cytometric analysis of FOLR2 transfectants labeling FOLR2 with ab234190 (red) compared with a negative control (blue). Surface staining.
Downloads
Product protocols
- Visit the general protocols
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