Rabbit Recombinant Monoclonal FOLR2 antibody. Carrier free. Suitable for IHC-P, ICC/IF and reacts with Human samples.
Images
![Western blot - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (AB302533), expandable thumbnail](https://content.abcam.com/products/images/folr2-antibody-epr25731-70-bsa-and-azide-free-ab302533--western-blot-img400922.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (AB302533)")
![Immunocytochemistry/ Immunofluorescence - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (AB302533), expandable thumbnail](https://content.abcam.com/products/images/folr2-antibody-epr25731-70-bsa-and-azide-free-ab302533--immunocytochemistry-immunofluorescence-img401024.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (AB302533)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (AB302533), expandable thumbnail](https://content.abcam.com/products/images/folr2-antibody-epr25731-70-bsa-and-azide-free-ab302533--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400960.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (AB302533)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (AB302533), expandable thumbnail](https://content.abcam.com/products/images/folr2-antibody-epr25731-70-bsa-and-azide-free-ab302533--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400959.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (AB302533)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (AB302533), expandable thumbnail](https://content.abcam.com/products/images/folr2-antibody-epr25731-70-bsa-and-azide-free-ab302533--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400958.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (AB302533)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt | IP | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release.
Alternative names
Folate receptor beta, FR-beta, Folate receptor 2, Placental folate-binding protein, FBP, FOLR2
Recommended products
Rabbit Recombinant Monoclonal FOLR2 antibody. Carrier free. Suitable for IHC-P, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25731-70
- Purification technique
- Affinity purification Protein A
- Specificity
We do not recommend this RabMAb for WB with endogenous lysates.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Do Not Freeze
Notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Folate receptor beta also known as FOLR2 is a protein with a molecular mass of approximately 32 kDa. It facilitates the transport of folate into cells. FOLR2 binds folate with high affinity and plays an important role in cellular metabolism. The receptor is mainly expressed in the placenta and certain myeloid cells such as macrophages and monocytes. Its expression is also notable in certain cancer cells but not in most normal tissues.
- Biological function summary
Folate receptor beta plays a significant role in folate uptake contributing to DNA synthesis and repair processes. FOLR2 as a part of the cell membrane forms a complex with other molecules to capture and internalize folate from the extracellular environment. This process is essential for the proliferation and survival of cells that require large amounts of folate including developing embryos and certain immune cells.
- Pathways
The function of folate receptor beta connects with the folate-mediated one-carbon metabolism pathway critical for nucleotide biosynthesis and methylation reactions. This pathway influences the synthesis of purines and pyrimidines supporting DNA replication and repair. FOLR2 interacts with folate transport proteins and enzymes involved in the methionine cycle which interlinks with amino acid metabolism and epigenetic regulation.
- Associated diseases and disorders
Folate receptor beta is associated with rheumatoid arthritis and ovarian cancer. In rheumatoid arthritis FOLR2 expression on activated macrophages indicates its potential as a biomarker and target for therapy. The overexpression of FOLR2 in ovarian cancer makes it a target for folate-linked therapeutic agents. The relationship between FOLR2 and immune proteins in inflammatory conditions highlights its role in modulating immune responses in disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533), expandable thumbnail](https://content.abcam.com/products/images/folr2-antibody-epr25731-70-bsa-and-azide-free-ab302533--western-blot-img400922.jpg "Western blot - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533)")
Western blot - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533)
This data was developed using Anti-FOLR2 antibody [EPR25731-70] ab302532, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody has no cross-reaction with FOLR1and FOLR3.All lanes: Western blot - Anti-FOLR2 antibody [EPR25731-70] (Anti-FOLR2 antibody [EPR25731-70] ab302532) at 1/1000 dilution
Lane 1: His-tagged human FOLR2 recombinant protein 10 ng
Lane 2: His-tagged human FOLR1 recombinant protein 10 ng
Lane 3: His-tagged human FOLR3 recombinant protein 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 38 kDa
Exposure time: 3min
![Immunocytochemistry/ Immunofluorescence - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533), expandable thumbnail](https://content.abcam.com/products/images/folr2-antibody-epr25731-70-bsa-and-azide-free-ab302533--immunocytochemistry-immunofluorescence-img401024.jpg "Immunocytochemistry/ Immunofluorescence - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533)")
Immunocytochemistry/ Immunofluorescence - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533)
This data was developed using Anti-FOLR2 antibody [EPR25731-70] ab302532, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling FOLR2 with Anti-FOLR2 antibody [EPR25731-70] ab302532 at 1/50 (10.28 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing membranous staining in THP-1 cells treated with Phorbol-12-myristate-13-acetate (80 nM) for 72 hours is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533), expandable thumbnail](https://content.abcam.com/products/images/folr2-antibody-epr25731-70-bsa-and-azide-free-ab302533--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400960.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533)
This data was developed using Anti-FOLR2 antibody [EPR25731-70] ab302532, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling FOLR2 with Anti-FOLR2 antibody [EPR25731-70] ab302532 at 1/100 (5.14 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on immune cells of human placenta (PMID: 24399840). The section was incubated with Anti-FOLR2 antibody [EPR25731-70] ab302532 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533), expandable thumbnail](https://content.abcam.com/products/images/folr2-antibody-epr25731-70-bsa-and-azide-free-ab302533--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400959.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533)
This data was developed using Anti-FOLR2 antibody [EPR25731-70] ab302532, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling FOLR2 with Anti-FOLR2 antibody [EPR25731-70] ab302532 at 1/100 (5.14 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on immune cells of human stomach. The section was incubated with Anti-FOLR2 antibody [EPR25731-70] ab302532 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533), expandable thumbnail](https://content.abcam.com/products/images/folr2-antibody-epr25731-70-bsa-and-azide-free-ab302533--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400958.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOLR2 antibody [EPR25731-70] (BSA and Azide free) (ab302533)
This data was developed using Anti-FOLR2 antibody [EPR25731-70] ab302532, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling FOLR2 with Anti-FOLR2 antibody [EPR25731-70] ab302532 at 1/100 (5.14 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on Kuffer cells of human liver. The section was incubated with Anti-FOLR2 antibody [EPR25731-70] ab302532 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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