Anti-FOLR2 antibody [EPR30335-548]

• WB

Lab

Western blot - Anti-FOLR2 antibody [EPR30335-548] (AB325399)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-FOLR2 antibody [EPR30335-548] (ab325399) at 1/1000 dilution

Lane 1:

Untreated J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) whole cell lysate at 20 µg

Lane 2:

J774A.1 treated with 10ng/ml M-CSF for 72 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 35 kDa,36 kDa

false

Exposure time: 26s

• WB

Lab

Western blot - Anti-FOLR2 antibody [EPR30335-548] (AB325399)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : skeletal muscle (PMID : 32532019).

The identity of the bands higher than 40 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lanes 1-2 : 180 seconds; Lanes 3-4 : 48 seconds.

All lanes:

Western blot - Anti-FOLR2 antibody [EPR30335-548] (ab325399) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 4:

3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 35 kDa,36 kDa

false

• Dot

Lab

Dot Blot - Anti-FOLR2 antibody [EPR30335-548] (AB325399)

Dot blot analysis of FOLR2 using ab325399 at 1 : 1000 (0.501 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) cells transfected with mouse FOLR1 expression vector containing a myc-His-tag® whole cell lysate

Lane 2 : HeLa cells transfected with mouse FOLR2 expression vector containing a myc-His-tag® whole cell lysate

Lane 3 : HeLa cells transfected with mouse FOLR4 expression vector containing a myc-His-tag® whole cell lysate

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed mouse FOLR1 or FOLR4 by dot blot.

All lanes:

Dot Blot - Anti-FOLR2 antibody [EPR30335-548] (ab325399) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) cells transfected with mouse FOLR1 expression vector containing a myc-His-tag® whole cell lysate

Lane 2:

HeLa cells transfected with mouse FOLR2 expression vector containing a myc-His-tag® whole cell lysate

Lane 3:

HeLa cells transfected with mouse FOLR4 expression vector containing a myc-His-tag® whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s