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Rabbit Monoclonal FOLR3 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Sandwich ELISA - Anti-FOLR3 antibody [EPR25732-32] - BSA and Azide free (Detector) (AB303705), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Isoform Short does not bind folate.

Alternative names

Folate receptor gamma, FR-gamma, Folate receptor 3, FOLR3

Recommended products

Rabbit Monoclonal FOLR3 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Alternative names

Folate receptor gamma, FR-gamma, Folate receptor 3, FOLR3

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR25732-32

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Anti-FOLR3 antibody [EPR25732-133] - BSA and Azide free (Capture) ab303704 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify human FLOR3. The recommended pair for sandwich ELISA is:

Capture: Anti-FOLR3 antibody [EPR25732-133] - BSA and Azide free (Capture) ab303704, human FLOR3 Capture Antibody (unconjugated)

Detector: ab303705, human FLOR3 Detector Antibody (unconjugated)

The reference range value is 15.6-2000 pg/ml.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

FOLR3 also known as folate receptor gamma is a member of the folate receptor family responsible for binding and transporting folate across cell membranes. This protein has a molecular mass of about 32 kDa. FOLR3 is primarily found in certain tissues including the placenta and liver and it exhibits limited expression in several epithelial tissues. Folate receptors including FOLR3 function by binding folates and delivering them into cells via endocytosis a process essential for the maintenance of regular cellular activity and function.

Biological function summary

Folate receptor gamma plays a significant role in the transport and uptake of folate an important vitamin necessary for DNA synthesis and methylation. The receptor assists in various cellular processes including nucleotide biosynthesis. FOLR3 works as part of a larger complex of folate receptors which ensure cells have adequate folate levels for normal metabolic functions. By doing so it helps facilitate normal cell growth and division critical in rapidly dividing cells.

Pathways

FOLR3 is notably involved in the folate metabolism pathway contributing to DNA synthesis and repair processes. This pathway connects with the one-carbon metabolism pathway where folate is an essential donor of carbons necessary for methylation reactions. In these pathways FOLR3 interacts with other folate receptors like FOLR1 and FOLR2 to maintain efficient folate transport and cellular homeostasis. Ensuring proper folate availability supports homocysteine metabolism which is another critical aspect of these pathways.

Associated diseases and disorders

FOLR3 relates notably to conditions such as cancer and inflammatory disorders. Cancer cells often exhibit elevated folate receptor expression including FOLR3 due to their increased requirement for folate in rapid cell proliferation. Inflammatory disorders also see changes in folate receptor expression which might affect immune cell functions and inflammation regulation. In these contexts FOLR3 interacts with tumor-associated proteins and immune modulators highlighting its potential as a therapeutic target in medical research and treatment development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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