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Mouse Monoclonal Fos B antibody. Suitable for Flow Cyt, WB, IHC-Fr and reacts with Human, Mouse samples. Cited in 44 publications. Immunogen corresponding to Synthetic Peptide within Human FOSB.


Images

Flow Cytometry - Anti-Fos B antibody [83B1138] (AB11959), expandable thumbnail
  • Western blot - Anti-Fos B antibody [83B1138] (AB11959), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Fos B antibody [83B1138] (AB11959), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human FOSB. The exact immunogen used to generate this antibody is proprietary information. Database link P53539

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-Fr
Human
Tested
Tested
Expected
Mouse
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1 µg for 106 Cells
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Heterodimerizes with proteins of the JUN family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to gene promoters containing an AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing their transcriptional activity (PubMed:12618758, PubMed:28981703). As part of the AP-1 complex, facilitates enhancer selection together with cell-type-specific transcription factors by collaboratively binding to nucleosomal enhancers and recruiting the SWI/SNF (BAF) chromatin remodeling complex to establish accessible chromatin (By similarity). Together with JUN, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed:12618758). Exhibits transactivation activity in vitro (By similarity). Involved in the display of nurturing behavior towards newborns (By similarity). May play a role in neurogenesis in the hippocampus and in learning and memory-related tasks by regulating the expression of various genes involved in neurogenesis, depression and epilepsy (By similarity). Implicated in behavioral responses related to morphine reward and spatial memory (By similarity). Isoform 11. Exhibits lower transactivation activity than isoform 1 in vitro (By similarity). The heterodimer with JUN does not display any transcriptional activity, and may thereby act as an transcriptional inhibitor (By similarity). May be involved in the regulation of neurogenesis in the hippocampus (By similarity). May play a role in synaptic modifications in nucleus accumbens medium spiny neurons and thereby play a role in adaptive and pathological reward-dependent learning, including maladaptive responses involved in drug addiction (By similarity). Seems to be more stably expressed with a half-life of ~9.5 hours in cell culture as compared to 1.5 hours half-life of isoform 1 (By similarity).

Alternative names

G0S3, FOSB, Protein FosB, G0/G1 switch regulatory protein 3, Transcription factor AP-1 subunit FosB

Recommended products

Mouse Monoclonal Fos B antibody. Suitable for Flow Cyt, WB, IHC-Fr and reacts with Human, Mouse samples. Cited in 44 publications. Immunogen corresponding to Synthetic Peptide within Human FOSB.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human FOSB. The exact immunogen used to generate this antibody is proprietary information. Database link P53539
Clone number
83B1138
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

Affinity purified.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Fos B also known as FosB is a protein encoded by the FOSB gene and belongs to the Fos family of transcription factors. Weighing approximately 46 kDa this protein acts within the cell nucleus and is expressed in several tissues particularly in brain regions. Delta Fos B an isoform of Fos B has gained attention for its stable accumulation in certain neuronal populations. Researchers often study Fos B using techniques like ELISA and immunohistochemistry to investigate its presence and distribution.

Biological function summary

Functions of Fos B include regulation of gene expression through forming AP-1 transcription factor complexes with Jun family proteins. These complexes bind to DNA influencing the expression of genes involved in processes such as cell proliferation differentiation and survival. Fos B is structurally similar to other Fos family proteins such as c-Fos which emphasises its role in response to extracellular signals and stress stimuli. Both Fos B and its isoform Delta Fos B have distinct expression patterns suggesting unique functions.

Pathways

Fos B plays a role in the MAPK/ERK signaling pathway which is essential for transmitting extracellular signals to promote diverse cellular responses. Within this pathway interactions with proteins like Jun lead to modulation of gene expression critical for cellular responses to growth factors. Additionally connections to the CREB signaling pathway link Fos B to processes concerning neuronal plasticity and memory further highlighting its participation in important biological functions.

Associated diseases and disorders

Involvement of Fos B has been noted in neurological conditions such as addiction and depression. Delta Fos B in particular accumulates in response to chronic drug exposure and serves as a molecular switch in addiction-related neuroadaptations. Connections with proteins such as CREB and other transcriptional regulators have broadened the understanding of how Fos B and its isoforms impact neural circuits and contribute to the pathology of mood disorders.

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3 product images

  • Flow Cytometry - Anti-Fos B antibody [83B1138] (ab11959), expandable thumbnail

    Flow Cytometry - Anti-Fos B antibody [83B1138] (ab11959)

    Overlay histogram showing HeLa cells stained with ab11959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11959, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-Fos B antibody [83B1138] (ab11959), expandable thumbnail

    Western blot - Anti-Fos B antibody [83B1138] (ab11959)

    ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB. ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB.

    All lanes: Western blot - Anti-Fos B antibody [83B1138] (ab11959)

    Predicted band size: 36 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Fos B antibody [83B1138] (ab11959), expandable thumbnail
    Image courtesy of Dr. Karine Thibault, Université Paris Descartes, France

    Immunohistochemistry (Frozen sections) - Anti-Fos B antibody [83B1138] (ab11959)

    ab11959 Fos B antibody immunostaining (red) in mouse brain cortex showing the striatum. Tissue was fixed with paraformaldehyde, before incubation with the antibody (1/3000) for 18 hours at 25°C. A donkey anti-mouse Alexa Fluor® 568 conjugated secondary was used at 1/1000.

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