Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (ab314068)

Rabbit Recombinant Monoclonal Fox2 / RBM9 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human RBFOX2 aa 1-50.

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Images

Flow Cytometry - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human RBFOX2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O43251

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt	ICC	IHC-P	IP	WB
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Target data

See full target information RBFOX2

Function

RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis (By similarity). RNA-binding protein that seems to act as a coregulatory factor of ER-alpha. Together with RNA binding proteins RBPMS and MBNL1/2, activates vascular smooth muscle cells alternative splicing events (PubMed:37548402).

Alternative names

FOX2, HRNBP2, RBM9, RTA, RBFOX2, RNA binding protein fox-1 homolog 2, Fox-1 homolog B, Hexaribonucleotide-binding protein 2, RNA-binding motif protein 9, RNA-binding protein 9, Repressor of tamoxifen transcriptional activity

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human RBFOX2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O43251
Clone number: BLR215K
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: Up to 12 months
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Fox2 also known as RBM9 is a protein involved in RNA splicing. It belongs to the Fox family of splicing factors and plays an important role in the regulation of alternative splicing. RBM9 has a mass of approximately 57 kDa. It is expressed in various tissues with notable expression in the brain heart and skeletal muscle. The presence of this protein in such tissues points to a role in neural muscular and cardiac function.

Biological function summary

RBM9 (Fox2) contributes to gene expression by influencing the splicing of pre-mRNA. It forms part of complex networks with other splicing-related proteins such as hnRNPs and SR proteins. Its function in alternative splicing impacts the diversity of protein isoforms which is important for cellular processes. This ability to modify gene expression patterns enables cellular adaptation to different physiological conditions.

Pathways

RBM9 interacts with key signaling cascades like PI3K/AKT and MAPK pathways. The protein aids in the regulation of genes involved in these pathways which are essential for cell growth and survival. RBM9 partners with other proteins such as AKT and ERK in these pathways facilitating their roles in cellular signaling. Its regulation of alternative splicing influences pathway outcomes contributing to normal and disease states.

Associated diseases and disorders

Malfunctions in RBM9 splicing regulation have links to neurodegenerative disorders like spinal muscular atrophy and cardiac conditions. RBM9's altered splicing patterns can result in disrupted neural and muscular functions. Its interactions with proteins such as SMN and MyoD connect RBM9 to these conditions indicating its influence on the severity and progression of related disorders. Understanding RBM9's role could offer insight into therapeutic targets for these diseases.

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9 product images

Flow Cytometry - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (ab314068)
Detection of mouse FOX2/RBM9 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody [BLR215K] (A700-215) or isotype control (unshaded). Secondary: 650-conjugated goat anti-rabbit IgG
Flow Cytometry - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (ab314068)
Detection of human FOX2/RBM9 (shaded) in Jurkat cells by flow cytometry. Antibody:Rabbit antiFOX2/RBM9 recombinant monoclonal antibody [BLR215K] (A700-215) or isotype control (unshaded). Secondary:DyLight® 650-conjugated goat anti-rabbit IgG (A120-101D5).
Western blot - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (ab314068)
Samples: Whole cell lysate (50 µg) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit antiFOX2/RBM9 recombinant monoclonal antibody [BLR215K] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody
All lanes: Western blot - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (ab314068) at 1/1000 dilution
Lane 1: NIH 3T3 Whole cell lysate at 50 µg
Lane 2: CT26 Whole cell lysate at 50 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Western blot - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (ab314068)
Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody [BLR215K] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody
All lanes: Western blot - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (ab314068) at 1/1000 dilution
Lane 1: HEK293T Whole cell lysate at 50 µg
Lane 2: HeLa Whole cell lysate at 50 µg
Lane 3: Hep-G2 Whole cell lysate at 50 µg
Lane 4: U2OS Whole cell lysate at 50 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Immunoprecipitation - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (ab314068)
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit antiFOX2/RBM9 recombinant monoclonal antibody [BLR215K] used for IP at 20 µl/mg lysate. FOX2/RBM9 was also immunoprecipitated by an antibody against a different epitope of FOX2/RBM9 (BL2440) and a polyclonal antibody against the same epitope of FOX2/RBM9 For blotting immunoprecipitated FOX2/RBM9, A700-215 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (ab314068)
Sample: FFPE section of NIH 3T3 cells. Antibody: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody [BLR215K] (A700-215CF). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (ab314068)
Sample: FFPE section of GaMG cells. Antibody: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody [BLR215K] (A700-215CF). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (ab314068)
Detection of mouse FOX2/RBM9 by immunohistochemistry. Sample: FFPE section of RENCA Tumor. Antibody: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody [BLR215K] (A700-215CF). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (ab314068)
Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody [BLR215K] (A700-215CF). Secondary: HRP-conjugated goat anti-rabbit IgG.