AB314068

Anti-FOX2/RBM9 antibody [BLR215K] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal Fox2 / RBM9 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human RBFOX2 aa 1-50.

View Alternative Names

FOX2, HRNBP2, RBM9, RTA, RBFOX2, RNA binding protein fox-1 homolog 2, Fox-1 homolog B, Hexaribonucleotide-binding protein 2, RNA-binding motif protein 9, RNA-binding protein 9, Repressor of tamoxifen transcriptional activity

9 Images

Flow Cytometry - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Detection of human FOX2/RBM9 (shaded) in Jurkat cells by flow cytometry. Antibody : Rabbit antiFOX2/RBM9 recombinant monoclonal antibody [BLR215K] (A700-215) or isotype control (unshaded). Secondary : DyLight® 650-conjugated goat anti-rabbit IgG (A120-101D5).

Supplier Data

Immunocytochemistry - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Sample : FFPE section of GaMG cells. Antibody : Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody [BLR215K] (A700-215CF). Secondary : HRP-conjugated goat anti-rabbit IgG.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Sample : FFPE section of breast carcinoma. Antibody : Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody [BLR215K] (A700-215CF). Secondary : HRP-conjugated goat anti-rabbit IgG.

Supplier Data

Immunoprecipitation - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies : Rabbit antiFOX2/RBM9 recombinant monoclonal antibody [BLR215K] used for IP at 20 µl/mg lysate. FOX2/RBM9 was also immunoprecipitated by an antibody against a different epitope of FOX2/RBM9 (BL2440) and a polyclonal antibody against the same epitope of FOX2/RBM9 For blotting immunoprecipitated FOX2/RBM9, A700-215 was used at 1 : 1000. Chemiluminescence with an exposure time of 30 seconds.

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Flow Cyt

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Flow Cytometry - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Detection of mouse FOX2/RBM9 (shaded) in EL4 cells by flow cytometry. Antibody : Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody [BLR215K] (A700-215) or isotype control (unshaded). Secondary : 650-conjugated goat anti-rabbit IgG

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Detection of mouse FOX2/RBM9 by immunohistochemistry. Sample : FFPE section of RENCA Tumor. Antibody : Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody [BLR215K] (A700-215CF). Secondary : HRP-conjugated goat anti-rabbit IgG.

Supplier Data

Immunocytochemistry - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Sample : FFPE section of NIH 3T3 cells. Antibody : Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody [BLR215K] (A700-215CF). Secondary : HRP-conjugated goat anti-rabbit IgG.

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Western blot - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Samples : Whole cell lysate (50 µg) from HEK293T, HeLa, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody : Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody [BLR215K] used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody

All lanes:

Western blot - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (ab314068) at 1/1000 dilution

Lane 1:

HEK293T Whole cell lysate at 50 µg

Lane 2:

HeLa Whole cell lysate at 50 µg

Lane 3:

Hep-G2 Whole cell lysate at 50 µg

Lane 4:

U2OS Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Supplier Data

Western blot - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Samples : Whole cell lysate (50 µg) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody : Rabbit antiFOX2/RBM9 recombinant monoclonal antibody [BLR215K] used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody

All lanes:

Western blot - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (ab314068) at 1/1000 dilution

Lane 1:

NIH 3T3 Whole cell lysate at 50 µg

Lane 2:

CT26 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR215K

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

Flow Cyt, IHC-P, WB, ICC, IP

applications

Immunogen

Synthetic Peptide within Human RBFOX2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O43251

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fox2 also known as RBM9 is a protein involved in RNA splicing. It belongs to the Fox family of splicing factors and plays an important role in the regulation of alternative splicing. RBM9 has a mass of approximately 57 kDa. It is expressed in various tissues with notable expression in the brain heart and skeletal muscle. The presence of this protein in such tissues points to a role in neural muscular and cardiac function.

Biological function summary

RBM9 (Fox2) contributes to gene expression by influencing the splicing of pre-mRNA. It forms part of complex networks with other splicing-related proteins such as hnRNPs and SR proteins. Its function in alternative splicing impacts the diversity of protein isoforms which is important for cellular processes. This ability to modify gene expression patterns enables cellular adaptation to different physiological conditions.

Pathways

RBM9 interacts with key signaling cascades like PI3K/AKT and MAPK pathways. The protein aids in the regulation of genes involved in these pathways which are essential for cell growth and survival. RBM9 partners with other proteins such as AKT and ERK in these pathways facilitating their roles in cellular signaling. Its regulation of alternative splicing influences pathway outcomes contributing to normal and disease states.

Malfunctions in RBM9 splicing regulation have links to neurodegenerative disorders like spinal muscular atrophy and cardiac conditions. RBM9's altered splicing patterns can result in disrupted neural and muscular functions. Its interactions with proteins such as SMN and MyoD connect RBM9 to these conditions indicating its influence on the severity and progression of related disorders. Understanding RBM9's role could offer insight into therapeutic targets for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis (By similarity). RNA-binding protein that seems to act as a coregulatory factor of ER-alpha. Together with RNA binding proteins RBPMS and MBNL1/2, activates vascular smooth muscle cells alternative splicing events (PubMed : 37548402).

See full target information RBFOX2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FOX2/RBM9 antibody [BLR215K] - BSA free

Flow Cytometry - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Immunocytochemistry - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Immunoprecipitation - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Flow Cytometry - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Immunocytochemistry - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Western blot - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Western blot - Anti-FOX2/RBM9 antibody [BLR215K] - BSA free (AB314068)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise