Rabbit Polyclonal FOXA1 antibody. Suitable for WB and reacts with Human samples. Cited in 167 publications.
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Publications
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- British journal of cancer 129:1903-19142023Fascin-1 expression is associated with neuroendocrine prostate cancer and directly suppressed by androgen receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnthony Turpin,Carine Delliaux,Pauline Parent,Hortense Chevalier,Carmen Escudero-Iriarte,Franck Bonardi,Nathalie Vanpouille,Anne Flourens,Jessica Querol,Aurélien Carnot,Xavier Leroy,Nicolás Herranz,Tristan Lanel,Arnauld Villers,Jonathan Olivier,Hélène Touzet,Yvan de Launoit,Tian V Tian,Martine Duterque-CoquillaudPubMed 37875732
- Journal of translational medicine 21:7162023Dissecting transcription of the 8q24-MYC locus in prostate cancer recognizes the equilibration between androgen receptor direct and indirect dual-functions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJu Guo,Zhao Wei,Tianwei Jia,Liyang Wang,Nuosu Nama,Jiaqian Liang,Xinghua Liao,Xiaming Liu,Yanfei Gao,Xiaoqiang Liu,Keshan Wang,Bin Fu,Shaoyong Shawn ChenPubMed 37828515
- Oncogene 42:1524-15422023Epigenomic mapping identifies an enhancer repertoire that regulates cell identity in bladder cancer through distinct transcription factor networks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHélène Neyret-Kahn,Jacqueline Fontugne,Xiang Yu Meng,Clarice S Groeneveld,Luc Cabel,Tao Ye,Elodie Guyon,Clémentine Krucker,Florent Dufour,Elodie Chapeaublanc,Audrey Rapinat,Daniel Jeffery,Laura Tanguy,Victoria Dixon,Yann Neuzillet,Thierry Lebret,David Gentien,Irwin Davidson,Yves Allory,Isabelle Bernard-Pierrot,François RadvanyiPubMed 36944729
- Cancer research 83:1684-16982023LSD1 Inhibition Disrupts Super-Enhancer-Driven Oncogenic Transcriptional Programs in Castration-Resistant Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuqing Li,Mingyu Liu,Wanting Han,Zifeng Wang,Dong Han,Susan Patalano,Jill A Macoska,Steven P Balk,Housheng Hansen He,Eva Corey,Shuai Gao,Changmeng CaiPubMed 36877164
- Nature communications 13:65752022FOXA1 repression drives lineage plasticity and immune heterogeneity in bladder cancers with squamous differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoshua I Warrick,Wenhuo Hu,Hironobu Yamashita,Vonn Walter,Lauren Shuman,Jenna M Craig,Lan L Gellert,Mauro A A Castro,A Gordon Robertson,Fengshen Kuo,Irina Ostrovnaya,Judy Sarungbam,Ying-Bei Chen,Anuradha Gopalan,Sahussapont J Sirintrapun,Samson W Fine,Satish K Tickoo,Kwanghee Kim,Jasmine Thomas,Nagar Karan,Sizhi Paul Gao,Timothy N Clinton,Andrew T Lenis,Timothy A Chan,Ziyu Chen,Manisha Rao,Travis J Hollman,Yanyun Li,Nicholas D Socci,Shweta Chavan,Agnes Viale,Neeman Mohibullah,Bernard H Bochner,Eugene J Pietzak,Min Yuen Teo,Gopa Iyer,Jonathan E Rosenberg,Dean F Bajorin,Matthew Kaag,Suzanne B Merrill,Monika Joshi,Rosalyn Adam,John A Taylor,Peter E Clark,Jay D Raman,Victor E Reuter,Yu Chen,Samuel A Funt,David B Solit,David J DeGraff,Hikmat A Al-AhmadiePubMed 36323682
- Cell reports 40:1114042022FOXA1 regulates alternative splicing in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarco Del Giudice,John G Foster,Serena Peirone,Alberto Rissone,Livia Caizzi,Federica Gaudino,Caterina Parlato,Francesca Anselmi,Rebecca Arkell,Simonetta Guarrera,Salvatore Oliviero,Giuseppe Basso,Prabhakar Rajan,Matteo CeredaPubMed 36170835
- Cancers 14:2022FOXA1 in Breast Cancer: A Luminal Marker with Promising Prognostic and Predictive Impact.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJasna Metovic,Fulvio Borella,Marta D'Alonzo,Nicoletta Biglia,Luca Mangherini,Cristian Tampieri,Luca Bertero,Paola Cassoni,Isabella CastellanoPubMed 36230619
- Cancer research 82:3673-36862022A Distinct Chromatin State Drives Therapeutic Resistance in Invasive Lobular Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgostina Nardone,Xintao Qiu,Sandor Spisak,Zsuzsanna Nagy,Ariel Feiglin,Avery Feit,Gabriela Cohen Feit,Yingtian Xie,Alba Font-Tello,Cristina Guarducci,Francisco Hermida-Prado,Sudeepa Syamala,Klothilda Lim,Miguel Munoz Gomez,Matthew Pun,MacIntosh Cornwell,Weihan Liu,Aysegul Ors,Hisham Mohammed,Paloma Cejas,Jane B Brock,Matthew L Freedman,Eric P Winer,Xiaoyong Fu,Rachel Schiff,Henry W Long,Otto Metzger Filho,Rinath JeselsohnPubMed 35950920
- Oncogene 41:4259-42702022FOXA1 inhibits hypoxia programs through transcriptional repression of HIF1A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohai Wang,Lourdes Brea,Xiaodong Lu,Galina Gritsina,Su H Park,Wanqing Xie,Jonathan C Zhao,Jindan YuPubMed 35931888
- JCI insight 7:2022Protein expression of the gp78 E3 ligase predicts poor breast cancer outcome based on race.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandeep K Singhal,Jung S Byun,Tingfen Yan,Ryan Yancey,Ambar Caban,Sara Gil Hernandez,Sediqua Bufford,Stephen M Hewitt,Joy Winfield,Jaya Pradhan,Vesco Mustkov,Jasmine A McDonald,Eliseo J Pérez-Stable,Anna María Nápoles,Nasreen Vohra,Adriana De Siervi,Clayton Yates,Melissa B Davis,Mei Yang,Yien Che Tsai,Allan M Weissman,Kevin GardnerPubMed 35639484
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | IHC-FoFr | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Predicted | Not recommended | Not recommended | Not recommended |
| Rat | Predicted | Not recommended | Not recommended | Not recommended |
| Xenopus laevis | Predicted | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.7-1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Xenopus laevis, Mouse | Dilution info - | Notes - |
IHC-FoFr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Xenopus laevis, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Xenopus laevis, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Xenopus laevis, Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promoter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
Alternative names
HNF3A, TCF3A, FOXA1, Hepatocyte nuclear factor 3-alpha, HNF-3-alpha, HNF-3A, Forkhead box protein A1, Transcription factor 3A, TCF-3A
Recommended products
Rabbit Polyclonal FOXA1 antibody. Suitable for WB and reacts with Human samples. Cited in 167 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FOXA1 also known as Forkhead Box A1 is a transcription factor with a molecular weight of about 49 kDa. It plays a role by binding to specific DNA sequences influencing the expression of genes involved in various cellular processes. This protein is expressed in tissues like liver lungs and prostate. Researchers also refer to FOXA1 as HNF-3α emphasizing its importance in transcription regulation.
- Biological function summary
FOXA1 influences the transcriptional control of genes essential for organ development and metabolic regulation. It acts as a pioneer factor opening chromatin structure to enable access for other transcription factors. FOXA1 integrates into complexes that involve histone modification affecting the chromatin state and transcription of target genes. This function is necessary for the proper differentiation and function of several tissues.
- Pathways
FOXA1 participates significantly in the androgen receptor signaling pathway and the estrogen receptor signaling pathway. It interacts closely with androgen receptor facilitating the expression of genes that influence cell growth and proliferation. The involvement of FOXA1 in these pathways highlights its critical role in pathways that regulate hormone-responsive cancers and metabolic processes.
- Associated diseases and disorders
FOXA1 shows a strong association with cancers such as prostate cancer and breast cancer. FOXA1's interaction with the androgen receptor links it to prostate cancer progression while its involvement with the estrogen receptor correlates with breast cancer development. Aberrations in its expression or function can disrupt these pathways emphasizing the importance of FOXA1 in cancer biology and its potential as a therapeutic target.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-FOXA1 antibody (ab23738)
Lanes 1 - 4: Merged signal (red and green). Green - ab23738 observed at 49 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.ab23738 was shown to recognize FOXA1 in wild-type HAP1 cells as signal was lost at the expected MW in FOXA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FOXA1 knockout samples were subjected to SDS-PAGE. ab23738 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 0.7 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-FOXA1 antibody (ab23738) at 0.7 µg/mL
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: FOXA1 knockout HAP1 whole cell lysate at 20 µg
Lane 3: HepG2 whole cell lysate at 20 µg
Lane 4: A549 whole cell lysate at 20 µg
Predicted band size: 49 kDa
Western blot - Anti-FOXA1 antibody (ab23738)
All lanes: Western blot - Anti-FOXA1 antibody (ab23738) at 1 µg/mL
All lanes: Human heart tissue lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 31 kDa, 51 kDa
Western blot - Anti-FOXA1 antibody (ab23738)
All lanes: Western blot - Anti-FOXA1 antibody (ab23738) at 1 µg/mL
All lanes: Recombinant Human FOXA1 protein (ab98301) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Exposure time: 10s
This image is a courtesy of Anonymous customer reviewWestern blot - Anti-FOXA1 antibody (ab23738)
All lanes: Western blot - Anti-FOXA1 antibody (ab23738) at 1/1000 dilution
Lane 1: Lysate prepared from human LNCaP cells at 20 µg
Lane 2: Lysate prepared from MCF7 cells at 20 µg
Lane 3: Lysate prepared from human LNCaP cells with ethanol as vehicle control at 20 µg
Lane 4: Lysate prepared from human LNCaP cells with 100 nM DHT at 20 µg
SecondaryAll lanes: HRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 51 kDa
Exposure time: 2s
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