AB173287

Anti-FOXA1 antibody [EPR10881-14]

Name: Anti-FOXA1 antibody [EPR10881-14] (ab173287) | Abcam
Brand: Abcam Limited
SKU: AB173287
Price: 345 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(10 Publications)

Rabbit Recombinant Monoclonal FOXA1 antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 10 publications.

View Alternative Names

HNF3A, TCF3A, FOXA1, Hepatocyte nuclear factor 3-alpha, HNF-3-alpha, HNF-3A, Forkhead box protein A1, Transcription factor 3A, TCF-3A

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (AB173287)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (AB173287)

IHC-Paraffin-embedded sections of Human prostate hyperplasia. 1 : 1000 dilution of ab173287 used. Counter stain = Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (AB173287)

IHC-Paraffin-embedded sections of Human breast carcinoma. 1 : 1000 dilution of ab173287 used. Counter stain = hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (AB173287)

ab173287 showing -ve staining in Human brain tissue. 1 : 1000 dilution of ab. Paraffin-embedded sections.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (AB173287)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (AB173287)

Left image : Immunofluorescent staining of SW480 cells using ab173287(1 : 1000 dilution). 1 : 200 dilution of Secondary antibody = Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078). Right image : Immunofluorescent staining of SW480 cells with DAPI. Fixative = 4% paraformaldehyde.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (AB173287)

Left image : Immunofluorescent staining of HepG2 cells using ab173287. 1 : 200 dilution of Secondary antibody = Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077). Right image : Immunofluorescent staining of HepG2 cells with DAPI. Fixative = 4% paraformaldehyde.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (AB173287)

IHC-Paraffin-embedded sections of Mouse colon. 1 : 1000 dilution of ab173287 used. Counter stain = Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Carrier free

Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10881-14

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 50% homology with FOXA2 and 81% homology with FOXA3. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2000", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2000", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXA1 also known as Forkhead Box A1 is a transcription factor with a molecular weight of about 49 kDa. It plays a role by binding to specific DNA sequences influencing the expression of genes involved in various cellular processes. This protein is expressed in tissues like liver lungs and prostate. Researchers also refer to FOXA1 as HNF-3α emphasizing its importance in transcription regulation.

Biological function summary

FOXA1 influences the transcriptional control of genes essential for organ development and metabolic regulation. It acts as a pioneer factor opening chromatin structure to enable access for other transcription factors. FOXA1 integrates into complexes that involve histone modification affecting the chromatin state and transcription of target genes. This function is necessary for the proper differentiation and function of several tissues.

Pathways

FOXA1 participates significantly in the androgen receptor signaling pathway and the estrogen receptor signaling pathway. It interacts closely with androgen receptor facilitating the expression of genes that influence cell growth and proliferation. The involvement of FOXA1 in these pathways highlights its critical role in pathways that regulate hormone-responsive cancers and metabolic processes.

FOXA1 shows a strong association with cancers such as prostate cancer and breast cancer. FOXA1's interaction with the androgen receptor links it to prostate cancer progression while its involvement with the estrogen receptor correlates with breast cancer development. Aberrations in its expression or function can disrupt these pathways emphasizing the importance of FOXA1 in cancer biology and its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promoter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.

See full target information FOXA1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Journal of respiratory biology and translational medicine 2: PubMed41049046

2025

Mitochondrial Lon Peptidase 1 Controls Diaphragm and Lung Development in a Context-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Le Xu,Chunting Tan,Nicole Talaba,Andrew Sou,Yufeng Shen,Wendy K Chung,David J McCulley,Xin Sun

Translational oncology 52:102269 PubMed39808845

2025

Molecular profiling of bladder cancer xenografts defines relevant molecular subtypes and provides a resource for biomarker discovery.

Applications

Unspecified application

Species

Unspecified reactive species

Sharada Mokkapati,Ganiraju Manyam,Alexis R Steinmetz,Côme Tholomier,Alberto Martini,Woonyoung Choi,Bogdon Czerniak,Byron H Lee,Colin P Dinney,David J McConkey

Cancer research 83:2839-2857 PubMed37272756

2023

Breast Cancer Mutations HER2V777L and PIK3CAH1047R Activate the p21-CDK4/6-Cyclin D1 Axis to Drive Tumorigenesis and Drug Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqing Cheng,Yirui Sun,Maureen Highkin,Nagalaxmi Vemalapally,Xiaohua Jin,Brandon Zhou,Julie L Prior,Ashley R Tipton,Shunqiang Li,Anton Iliuk,Samuel Achilefu,Ian S Hagemann,John R Edwards,Ron Bose

Cancers 14: PubMed36230619

2022

FOXA1 in Breast Cancer: A Luminal Marker with Promising Prognostic and Predictive Impact.

Applications

Unspecified application

Species

Unspecified reactive species

Jasna Metovic,Fulvio Borella,Marta D'Alonzo,Nicoletta Biglia,Luca Mangherini,Cristian Tampieri,Luca Bertero,Paola Cassoni,Isabella Castellano

Developmental cell 57:1866-1882.e10 PubMed35835117

2022

FoxA1 and FoxA2 control growth and cellular identity in NKX2-1-positive lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Grace Orstad,Gabriela Fort,Timothy J Parnell,Alex Jones,Chris Stubben,Brian Lohman,Katherine L Gillis,Walter Orellana,Rushmeen Tariq,Olaf Klingbeil,Klaus Kaestner,Christopher R Vakoc,Benjamin T Spike,Eric L Snyder

Ginekologia polska 92:544-549 PubMed33844251

2021

FOXA1 is associated with high tumor grade, myometrial invasion and lymph node invasion in endometrial endometrioid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hülya Tosun Yıldırım,Döndü Nergiz,Canan Sadullahoğlu,Dinç Süren,Şenay Yıldırım,Işın Üreyen,Cem Sezer

The American journal of pathology 190:484-502 PubMed31843498

2019

Src Kinase Is Biphosphorylated at Y416/Y527 and Activates the CUB-Domain Containing Protein 1/Protein Kinase C δ Pathway in a Subset of Triple-Negative Breast Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Luke J Nelson,Heather J Wright,Nguyen B Dinh,Kevin D Nguyen,Olga V Razorenova,F Scott Heinemann

Nature communications 10:4407 PubMed31562298

2019

Urothelial organoids originating from Cd49f mouse stem cells display Notch-dependent differentiation capacity.

Applications

Unspecified application

Species

Unspecified reactive species

Catarina P Santos,Eleonora Lapi,Jaime Martínez de Villarreal,Laura Álvaro-Espinosa,Asunción Fernández-Barral,Antonio Barbáchano,Orlando Domínguez,Ashley M Laughney,Diego Megías,Alberto Muñoz,Francisco X Real

eLife 7: PubMed30475207

2018

FoxA1 and FoxA2 drive gastric differentiation and suppress squamous identity in NKX2-1-negative lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Soledad A Camolotto,Shrivatsav Pattabiraman,Timothy L Mosbruger,Alex Jones,Veronika K Belova,Grace Orstad,Mitchell Streiff,Lydia Salmond,Chris Stubben,Klaus H Kaestner,Eric L Snyder

Neoplasma 65:469-476 PubMed29788741

2018

FOXA1 and CK7 expression in esophageal squamous cell carcinoma and its prognostic significance.

Applications

Unspecified application

Species

Unspecified reactive species

Y Xu,W Wang,L Li,J Liu,X Wu,J Yu,H Wang,W Cui,R Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FOXA1 antibody [EPR10881-14]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (AB173287)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (AB173287)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (AB173287)

Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (AB173287)

Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (AB173287)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (AB173287)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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