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Rabbit Recombinant Monoclonal FOXA1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 38 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PChIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Tested
Mouse
Tested
Not recommended
Tested
Expected
Expected
Rat
Tested
Not recommended
Tested
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

For unpurified use 1/100 - 1/250

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

For unpurified use 1/100 - 1/250

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

For unpurified use 1/100 - 1/250

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promoter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.

Alternative names

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Rabbit Recombinant Monoclonal FOXA1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 38 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR10881

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological summary

FOXA1 influences the transcriptional control of genes essential for organ development and metabolic regulation. It acts as a pioneer factor opening chromatin structure to enable access for other transcription factors. FOXA1 integrates into complexes that involve histone modification affecting the chromatin state and transcription of target genes. This function is necessary for the proper differentiation and function of several tissues.

Mechanical summary

FOXA1 also known as Forkhead Box A1 is a transcription factor with a molecular weight of about 49 kDa. It plays a role by binding to specific DNA sequences influencing the expression of genes involved in various cellular processes. This protein is expressed in tissues like liver lungs and prostate. Researchers also refer to FOXA1 as HNF-3α emphasizing its importance in transcription regulation.

Pathway

FOXA1 participates significantly in the androgen receptor signaling pathway and the estrogen receptor signaling pathway. It interacts closely with androgen receptor facilitating the expression of genes that influence cell growth and proliferation. The involvement of FOXA1 in these pathways highlights its critical role in pathways that regulate hormone-responsive cancers and metabolic processes.

Disease

FOXA1 shows a strong association with cancers such as prostate cancer and breast cancer. FOXA1's interaction with the androgen receptor links it to prostate cancer progression while its involvement with the estrogen receptor correlates with breast cancer development. Aberrations in its expression or function can disrupt these pathways emphasizing the importance of FOXA1 in cancer biology and its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Western blot - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933), expandable thumbnail

    Western blot - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933)

    Lanes 1 - 4: Merged signal (red and green). Green - ab170933 observed at 52 kDa. Red - loading control, ab9484, observed at 37 kDa.

    Unpurified ab170933 was shown to specifically react with FOXA1 in wild-type HAP1 cells as signal was lost in FOXA1 knockout cells. Wild-type and FOXA1 knockout samples were subjected to SDS-PAGE. Ab170933 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (AB170933) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: FOXA1 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: MCF7 whole cell lysate at 20 µg

    Lane 4: HepG2 whole cell lysate at 20 µg

    Predicted band size: 49 kDa

  • Western blot - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933), expandable thumbnail

    Western blot - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (AB170933) at 1.1 µg/mL

    Lane 1: SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Lane 2: Mouse lung lysates at 15 µg

    Lane 3: Rat lung lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 49 kDa

    Observed band size: 49 kDa

  • Western blot - Anti-FOXA1 antibody [EPR10881] (ab170933), expandable thumbnail

    Western blot - Anti-FOXA1 antibody [EPR10881] (ab170933)

    Lanes 1- 2: Merged signal (red and green). Green - ab170933 observed at 52 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

    ab170933 was shown to react with FOXA1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261823 (knockout cell lysate ab256920) was used. Wild-type HeLa and FOXA1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab170933 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-FOXA1 antibody [EPR10881] (AB170933) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: FOXA1 knockout HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 49 kDa

    Observed band size: 52 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933)

    Immunocytochemistry/ Immunofluorescence analysis of PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling FOXA1 with Purified ab170933 at 1:100 dilution (11 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat pancreas tissue sections labeling FOXA1 with Purified ab170933 at 1:1000 dilution (1.13 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling FOXA1 with Purified ab170933 at 1:1000 dilution (1.13 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling FOXA1 with Purified ab170933 at 1:1000 dilution (1.13 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933)

    Immunofluorescence analysis of HepG2 cells labeling FOXA1 with unpurified ab170933 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling FOXA1 with unpurified ab170933 at 1/100 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933)

    Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling FOXA1 with unpurified ab170933 at 1/100 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-FOXA1 antibody [EPR10881] (ab170933), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-FOXA1 antibody [EPR10881] (ab170933)

    Intracellular Flow Cytometry analysis of PC-3 (human prostate adenocarcinoma epithelial cell) cells labeling FOXA1 with purified ab170933 at 1/50 dilution (1 ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) (1/5000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

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