Name: Anti-FOXA1 antibody [EPR10881]
SKU: ab170933
Rating: 5 (1 reviews)

Anti-FOXA1 antibody [EPR10881] (ab170933) is a rabbit monoclonal antibody that is used to detect FOXA1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.

- Specificity confirmed with FOXA1 knockout cell line validation

Images

Western blot - Anti-FOXA1 antibody [EPR10881] (AB170933), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ChIP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Not recommended	Tested	Tested	Tested
Mouse	Tested	Not recommended	Tested	Expected	Expected
Rat	Tested	Not recommended	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes For unpurified use 1/100 - 1/250 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes For unpurified use 1/100 - 1/250 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes For unpurified use 1/100 - 1/250 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/10000	Notes -
Species Rat	Dilution info 1/1000 - 1/10000	Notes -
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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2 products for Alternative Version

Target data

See full target information FOXA1

Function

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promoter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.

Alternative names

HNF3A, TCF3A, FOXA1, Hepatocyte nuclear factor 3-alpha, HNF-3-alpha, HNF-3A, Forkhead box protein A1, Transcription factor 3A, TCF-3A

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR10881
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-FOXA1 antibody [EPR10881] (ab170933) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of FOXA1?

Anti-FOXA1 [EPR10881] (ab170933) specifically detects a band for FOXA1 (UniProt: P55317) at a molecular weight of 49kDa.

Trusted by the scientific community

Anti-FOXA1 [EPR10881] (ab170933) was first used in a scientific publication in 2013 and has been cited over 30 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed

The specificity of Anti-FOXA1 antibody [EPR10881] (ab170933) has been confirmed by Western blot testing in FOXA1 Knockout HAP1 cell line, ab261823.

Other related products

We have a range of other formats of antibody clone [EPR10881] also available for your convenience:
ab170933, Alexa Fluor® 647 - ab196908, Alexa Fluor® 488 - ab197235, Carrier free - ab236011

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

FOXA1 also known as Forkhead Box A1 is a transcription factor with a molecular weight of about 49 kDa. It plays a role by binding to specific DNA sequences influencing the expression of genes involved in various cellular processes. This protein is expressed in tissues like liver lungs and prostate. Researchers also refer to FOXA1 as HNF-3α emphasizing its importance in transcription regulation.

Biological function summary

FOXA1 influences the transcriptional control of genes essential for organ development and metabolic regulation. It acts as a pioneer factor opening chromatin structure to enable access for other transcription factors. FOXA1 integrates into complexes that involve histone modification affecting the chromatin state and transcription of target genes. This function is necessary for the proper differentiation and function of several tissues.

Pathways

FOXA1 participates significantly in the androgen receptor signaling pathway and the estrogen receptor signaling pathway. It interacts closely with androgen receptor facilitating the expression of genes that influence cell growth and proliferation. The involvement of FOXA1 in these pathways highlights its critical role in pathways that regulate hormone-responsive cancers and metabolic processes.

Associated diseases and disorders

FOXA1 shows a strong association with cancers such as prostate cancer and breast cancer. FOXA1's interaction with the androgen receptor links it to prostate cancer progression while its involvement with the estrogen receptor correlates with breast cancer development. Aberrations in its expression or function can disrupt these pathways emphasizing the importance of FOXA1 in cancer biology and its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

Western blot - Anti-FOXA1 antibody [EPR10881] (ab170933)
Lanes 1 - 4: Merged signal (red and green). Green - ab170933 observed at 52 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Unpurified ab170933 was shown to specifically react with FOXA1 in wild-type HAP1 cells as signal was lost in FOXA1 knockout cells. Wild-type and FOXA1 knockout samples were subjected to SDS-PAGE. ab170933 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-FOXA1 antibody [EPR10881] (ab170933) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: FOXA1 knockout HAP1 whole cell lysate at 20 µg
Lane 3: MCF7 whole cell lysate at 20 µg
Lane 4: HepG2 whole cell lysate at 20 µg
Predicted band size: 49 kDa
Western blot - Anti-FOXA1 antibody [EPR10881] (ab170933)
Blocking and diluting buffer: 5% NFDM/TBST.

All lanes: Western blot - Anti-FOXA1 antibody [EPR10881] (ab170933) at 1.1 µg/mL
Lane 1: SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2: Mouse lung lysates at 15 µg
Lane 3: Rat lung lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Western blot - Anti-FOXA1 antibody [EPR10881] (ab170933)
Lanes 1- 2: Merged signal (red and green). Green - ab170933 observed at 52 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
ab170933 was shown to react with FOXA1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human FOXA1 knockout HeLa cell line ab261823 (knockout cell lysate Human FOXA1 knockout HeLa cell lysate ab256920) was used. Wild-type HeLa and FOXA1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab170933 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-FOXA1 antibody [EPR10881] (ab170933) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: FOXA1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human FOXA1 knockout HeLa cell line (Human FOXA1 knockout HeLa cell line ab261823)
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 52 kDa
Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881] (ab170933)
Immunocytochemistry/ Immunofluorescence analysis of PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling FOXA1 with Purified ab170933 at 1:100 dilution (11 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] (ab170933)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat pancreas tissue sections labeling FOXA1 with Purified ab170933 at 1:1000 dilution (1.13 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] (ab170933)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling FOXA1 with Purified ab170933 at 1:1000 dilution (1.13 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] (ab170933)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling FOXA1 with Purified ab170933 at 1:1000 dilution (1.13 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881] (ab170933)
Immunofluorescence analysis of HepG2 cells labeling FOXA1 with unpurified ab170933 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] (ab170933)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling FOXA1 with unpurified ab170933 at 1/100 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881] (ab170933)
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling FOXA1 with unpurified ab170933 at 1/100 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-FOXA1 antibody [EPR10881] (ab170933)
Intracellular Flow Cytometry analysis of PC-3 (human prostate adenocarcinoma epithelial cell) cells labeling FOXA1 with purified ab170933 at 1/50 dilution (1 ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) (1/5000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.