Anti-FOXA2 [7E6] antibody (ab60721) is a mouse monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect FOXA2 in Western Blot, Flow Cytometry, IP, ICC/IF. Suitable for Human samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Images
![Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721), expandable thumbnail](https://content.abcam.com/products/images/foxa2-antibody-7e6-bsa-and-azide-free-ab60721--immunocytochemistry-immunofluorescence-img10318.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721)")
![Flow Cytometry - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721), expandable thumbnail](https://content.abcam.com/products/images/foxa2-antibody-7e6-bsa-and-azide-free-ab60721--flow-cytometry-img56713.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721)")
![Western blot - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721), expandable thumbnail](https://content.abcam.com/products/images/foxa2-antibody-7e6-bsa-and-azide-free-ab60721--western-blot-img161744.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721)")
![Immunoprecipitation - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721), expandable thumbnail](https://content.abcam.com/products/images/foxa2-antibody-7e6-bsa-and-azide-free-ab60721--immunoprecipitation-img143856.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721)")
Publications
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- iScience 26:1082052023Human pluripotent stem cell fate trajectories toward lung and hepatocyte progenitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaido Ori,Meshal Ansari,Ilias Angelidis,Ruth Olmer,Ulrich Martin,Fabian J Theis,Herbert B Schiller,Micha DrukkerPubMed 38026193
- Scientific data 10:932023A dataset of definitive endoderm and hepatocyte differentiations from human induced pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuki Tanaka,Erina Furuhata,Shiori Maeda,Mami Kishima,Harukazu Suzuki,Takahiro SuzukiPubMed 36788249
- eLife 11:2022Contribution of -labeled cells to alveolar regeneration is independent of tuft cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuachao Huang,Yinshan Fang,Ming Jiang,Yihan Zhang,Jana Biermann,Johannes C Melms,Jennifer A Danielsson,Ying Yang,Li Qiang,Jia Liu,Yiwu Zhou,Manli Wang,Zhihong Hu,Timothy C Wang,Anjali Saqi,Jie Sun,Ichiro Matsumoto,Wellington V Cardoso,Charles W Emala,Jian Zhu,Benjamin Izar,Hongmei Mou,Jianwen QuePubMed 36129169
- Stem cell research 64:1028892022Reprogramming of dermal fibroblasts from a Duchenne muscular dystrophy patient carrying a deletion of exons 45-50 into an induced pluripotent stem cell line (CCMi005-A).Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavide Rovina,Elisa Castiglioni,Sara Mallia,Martina Rabino,Andrea Farini,Marzia Belicchi,Giusy Di Giuseppe,Cristina Gervasini,Yvan Torrente,Giulio Pompilio,Aoife GowranPubMed 35961103
- Stem cell research 61:1027582022Characterization of an induced pluripotent stem cell line (UMi040-A) bearing an auditory neuropathy spectrum disorder-associated variant in TMEM43.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPei-Ciao Tang,Marie V Roche,Se Young Um,Nicholas C Gosstola,Min Young Kim,Byung Yoon Choi,Derek M Dykxhoorn,Xue Zhong LiuPubMed 35364395
- Frontiers in oncology 11:6050252021FOXA2-Interacting FOXP2 Prevents Epithelial-Mesenchymal Transition of Breast Cancer Cells by Stimulating E-Cadherin and PHF2 Transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuxiang Liu,Taolin Chen,Mingyue Guo,Yu Li,Qian Zhang,Guixiang Tan,Li Yu,Yongjun TanPubMed 33718155
- Journal of cellular and molecular medicine :2020A functional genomics approach to investigate the differentiation of iPSCs into lung epithelium at air-liquid interface.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJenny L Kerschner et. al.PubMed 32692488
- Development, growth & differentiation 62:243-2592020Modeling early stages of endoderm development in epiblast stem cell aggregates with supply of extracellular matrices.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSachiko Inamori et. al.PubMed 32277710
- Evidence-based complementary and alternative medic 2020:91041352020Plastrum Testudinis Extracts Promote NSC Differentiation into Dopaminergic Neuron by Regulating the Interaction of TET1 and FoxA2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Zhong et. al.PubMed 32382312
- Bioscience reports 39:2019MiR-942 regulates the function of breast cancer cell by targeting FOXA2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinku Zhang,Zhiqiang Zhang,Jirui Sun,Qiushuang Ma,Wenming Zhao,Xue Chen,Haizhi QiaoPubMed 31701999
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human FOXA2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y261
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted | Predicted |
| Transfected cell lysate | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog, Rhesus monkey | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog, Rhesus monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog, Rhesus monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog, Rhesus monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
Alternative names
HNF3B, TCF3B, FOXA2, Hepatocyte nuclear factor 3-beta, HNF-3-beta, HNF-3B, Forkhead box protein A2, Transcription factor 3B, TCF-3B
Recommended products
Anti-FOXA2 [7E6] antibody (ab60721) is a mouse monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect FOXA2 in Western Blot, Flow Cytometry, IP, ICC/IF. Suitable for Human samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human FOXA2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y261
- Carrier free
- Yes
- Clone number
- 7E6
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of FOXA2?
Anti-FOXA2 [7E6] - BSA and Azide free (ab60721) specifically detects a band for FOXA2 (UniProt: Q9Y261) at a molecular weight of 48kDa.
Trusted by the scientific community
Anti-FOXA2 [7E6] - BSA and Azide free (ab60721) was first used in a scientific publication in 2007 and has been cited over 20 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FOXA2 also known as HNF3B is a transcription factor that plays a significant role in gene regulation. This protein has a mass of approximately 47 kDa and is expressed in various tissues including the liver pancreas and lung. Researchers detect FOXA2 using techniques like ELISA for protein quantification. Its function involves regulating the expression of genes critical for development and metabolism.
- Biological function summary
FOXA2 influences important processes in embryonic development and organogenesis. It does not typically act as part of a larger complex but independently binds to DNA enabling the transcription of target genes. FOXA2 affects liver function by regulating genes involved in glucose and lipid metabolism and it also plays a part in the differentiation of cells in development stages.
- Pathways
FOXA2 operates within pathways like the insulin signaling pathway and the Wnt signaling pathway. It collaborates with other transcription factors like FOXA1 and FOXA3 to maintain metabolic homeostasis. In the Wnt pathway FOXA2 impacts cell fate decisions essential for embryonic patterning and development.
- Associated diseases and disorders
Alterations in FOXA2 expression have connections to conditions such as diabetes and liver diseases. Changes in FOXA2 expression could disrupt liver metabolism leading to metabolic disorders. Moreover FOXA2 ties to the regulation of other proteins like PDX1 in the context of diabetes where it plays a role in insulin biosynthesis and pancreatic cell function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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Full details and terms and conditions can be found here:
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4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721), expandable thumbnail](https://content.abcam.com/products/images/foxa2-antibody-7e6-bsa-and-azide-free-ab60721--immunocytochemistry-immunofluorescence-img10318.jpg "Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721)")
Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721)
ab60721 at 10 ug/ml staining FOXA2 in human Hella cells by Immunocytochemistry/ Immunofluorescence.
This image was generated using the ascites version of the product.
![Flow Cytometry - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721), expandable thumbnail](https://content.abcam.com/products/images/foxa2-antibody-7e6-bsa-and-azide-free-ab60721--flow-cytometry-img56713.jpg "Flow Cytometry - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721)")
Flow Cytometry - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721)
Overlay histogram showing HepG2 cells stained with ab60721 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60721, 0.5μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
![Western blot - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721), expandable thumbnail](https://content.abcam.com/products/images/foxa2-antibody-7e6-bsa-and-azide-free-ab60721--western-blot-img161744.jpg "Western blot - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721)")
Western blot - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721)
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721) at 1 µg/mL
All lanes: HepG2 cell lysate at 25 µg
Predicted band size: 48 kDa
Observed band size: 40 kDa, 42 kDa, 50 kDa
![Immunoprecipitation - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721), expandable thumbnail](https://content.abcam.com/products/images/foxa2-antibody-7e6-bsa-and-azide-free-ab60721--immunoprecipitation-img143856.jpg "Immunoprecipitation - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721)")
Immunoprecipitation - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721)
Immunoprecipitation analysis of FOXA2 transfected lysate using ab60721 and protein A magnetic bead.
This image was generated using the ascites version of the product.
All lanes: Immunoprecipitation - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721)
Predicted band size: 48 kDa
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