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Rabbit Recombinant Monoclonal FOXA2 antibody. Suitable for ChIP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

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Images

ChIP - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (AB108396), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (AB108396), expandable thumbnail
  • Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (AB108396), expandable thumbnail
  • Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (AB108396), expandable thumbnail
  • Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (AB108396), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPWBICC/IF
Human
Tested
Tested
Tested
Mouse
Predicted
Expected
Predicted
Rat
Predicted
Expected
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/300 - 1/600
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.

Alternative names

HNF3B, TCF3B, FOXA2, Hepatocyte nuclear factor 3-beta, HNF-3-beta, HNF-3B, Forkhead box protein A2, Transcription factor 3B, TCF-3B

Recommended products

Rabbit Recombinant Monoclonal FOXA2 antibody. Suitable for ChIP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4465
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FOXA2 also known as HNF3B is a transcription factor that plays a significant role in gene regulation. This protein has a mass of approximately 47 kDa and is expressed in various tissues including the liver pancreas and lung. Researchers detect FOXA2 using techniques like ELISA for protein quantification. Its function involves regulating the expression of genes critical for development and metabolism.

Biological function summary

FOXA2 influences important processes in embryonic development and organogenesis. It does not typically act as part of a larger complex but independently binds to DNA enabling the transcription of target genes. FOXA2 affects liver function by regulating genes involved in glucose and lipid metabolism and it also plays a part in the differentiation of cells in development stages.

Pathways

FOXA2 operates within pathways like the insulin signaling pathway and the Wnt signaling pathway. It collaborates with other transcription factors like FOXA1 and FOXA3 to maintain metabolic homeostasis. In the Wnt pathway FOXA2 impacts cell fate decisions essential for embryonic patterning and development.

Associated diseases and disorders

Alterations in FOXA2 expression have connections to conditions such as diabetes and liver diseases. Changes in FOXA2 expression could disrupt liver metabolism leading to metabolic disorders. Moreover FOXA2 ties to the regulation of other proteins like PDX1 in the context of diabetes where it plays a role in insulin biosynthesis and pancreatic cell function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • ChIP - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396), expandable thumbnail

    ChIP - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)

    Chromatin was prepared from HepG2 cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab108396 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)

    Immunofluorescence staining of HepG2 cells with purified ab108396 at a working dilution of 1 in 300, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) . The secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab108396 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (ab15007) were used.

  • Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396), expandable thumbnail

    Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396) at 1/1000 dilution

    All lanes: HepG2 cell lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 110 kDa, 48 kDa

    Observed band size: 52 kDa, 98 kDa

  • Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396), expandable thumbnail

    Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396) at 1/5000 dilution

    All lanes: SW480 cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa, 48 kDa, 72 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396), expandable thumbnail

    Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)

    All lanes: Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396) at 1/1000 dilution

    Lane 1: HepG2 lysate at 10 µg

    Lane 2: Human colon cancer lysate at 10 µg

    Predicted band size: 47 kDa, 48 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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